Restriction map for
pF1KB7032
(Linear) (Six-base) MAPSORT of: KIBB7032.seq Check: 1406 from: 1 to: 1272
KIBB7032.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
Acc65I G'GTAC_C
Cuts at: 0 1218 1272
Size: 1218 54
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 728 1272
Size: 728 544
AleI CACnn'nnGTG
Cuts at: 0 190 1272
Size: 190 1082
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 268 1272
Size: 268 1004
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1192 1224 1272
Size: 1192 32 48
Fragments arranged by size:
1192 48 32
AlwNI CAG_nnn'CTG
Cuts at: 0 847 1272
Size: 847 425
ApoI r'AATT_y
Cuts at: 0 1021 1131 1272
Size: 1021 110 141
Fragments arranged by size:
1021 141 110
AvaI C'yCGr_G
Cuts at: 0 1064 1272
Size: 1064 208
BanI G'GyrC_C
Cuts at: 0 206 1218 1272
Size: 206 1012 54
Fragments arranged by size:
1012 206 54
BbeI G_GCGC'C
Cuts at: 0 210 1272
Size: 210 1062
BbvCI CC'TCA_GC
Cuts at: 0 944 1272
Size: 944 328
BclI T'GATC_A
Cuts at: 0 500 656 794 1272
Size: 500 156 138 478
Fragments arranged by size:
500 478 156 138
Bme1580I G_kGCm'C
Cuts at: 0 1065 1272
Size: 1065 207
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 106 1272
Size: 106 1166
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 466 1226 1272
Size: 466 760 46
Fragments arranged by size:
760 466 46
Bpu10I CC'TnA_GC
Cuts at: 0 545 944 1272
Size: 545 399 328
Fragments arranged by size:
545 399 328
BsaI GGTCTCn'nnnn_
Cuts at: 0 77 1272
Size: 77 1195
BsaAI yAC'GTr
Cuts at: 0 807 1272
Size: 807 465
BsaHI Gr'CG_yC
Cuts at: 0 207 1272
Size: 207 1065
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 43 961 1191 1272
Size: 43 918 230 81
Fragments arranged by size:
918 230 81 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1077 1272
Size: 1077 195
BsmI GAATG_Cn'
Cuts at: 0 457 1272
Size: 457 815
Bsp1286I G_dGCh'C
Cuts at: 0 1065 1272
Size: 1065 207
BspHI T'CATG_A
Cuts at: 0 533 1272
Size: 533 739
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 71 1272
Size: 71 1201
BsrBI CCG'CTC
Cuts at: 0 119 1272
Size: 119 1153
BsrDI GCAATG_nn'
Cuts at: 0 938 1272
Size: 938 334
BssHII G'CGCG_C
Cuts at: 0 43 1272
Size: 43 1229
BstAPI GCAn_nnn'nTGC
Cuts at: 0 617 1272
Size: 617 655
BstEII G'GTnAC_C
Cuts at: 0 637 1272
Size: 637 635
BstXI CCAn_nnnn'nTGG
Cuts at: 0 192 1272
Size: 192 1080
BstYI r'GATC_y
Cuts at: 0 950 1272
Size: 950 322
Bsu36I CC'TnA_GG
Cuts at: 0 643 1272
Size: 643 629
BtgI C'CryG_G
Cuts at: 0 291 1051 1272
Size: 291 760 221
Fragments arranged by size:
760 291 221
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 33 93 1272
Size: 33 60 1179
Fragments arranged by size:
1179 60 33
BtsI GCAGTG_nn'
Cuts at: 0 1065 1272
Size: 1065 207
DraIII CAC_nnn'GTG
Cuts at: 0 356 1272
Size: 356 916
DrdI GACnn_nn'nnGTC
Cuts at: 0 871 1272
Size: 871 401
EaeI y'GGCC_r
Cuts at: 0 5 461 854 1272
Size: 5 456 393 418
Fragments arranged by size:
456 418 393 5
EarI CTCTTCn'nnn_
Cuts at: 0 262 400 1272
Size: 262 138 872
Fragments arranged by size:
872 262 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 466 728 1226 1272
Size: 466 262 498 46
Fragments arranged by size:
498 466 262 46
EcoO109I rG'GnC_Cy
Cuts at: 0 122 1272
Size: 122 1150
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 636 1272
Size: 636 636
FspI TGC'GCA
Cuts at: 0 67 676 1272
Size: 67 609 596
Fragments arranged by size:
609 596 67
FspAI rTGC'GCAy
Cuts at: 0 676 1272
Size: 676 596
HaeII r_GCGC'y
Cuts at: 0 210 1272
Size: 210 1062
KasI G'GCGC_C
Cuts at: 0 206 1272
Size: 206 1066
KpnI G_GTAC'C
Cuts at: 0 1222 1272
Size: 1222 50
MscI TGG'CCA
Cuts at: 0 7 463 856 1272
Size: 7 456 393 416
Fragments arranged by size:
456 416 393 7
MslI CAynn'nnrTG
Cuts at: 0 190 532 682 1272
Size: 190 342 150 590
Fragments arranged by size:
590 342 190 150
MspA1I CmG'CkG
Cuts at: 0 248 1272
Size: 248 1024
NarI GG'CG_CC
Cuts at: 0 207 1272
Size: 207 1065
NcoI C'CATG_G
Cuts at: 0 291 1272
Size: 291 981
PflMI CCAn_nnn'nTGG
Cuts at: 0 216 867 1272
Size: 216 651 405
Fragments arranged by size:
651 405 216
PfoI T'CCnGG_A
Cuts at: 0 23 1272
Size: 23 1249
PmlI CAC'GTG
Cuts at: 0 807 1272
Size: 807 465
PshAI GACnn'nnGTC
Cuts at: 0 367 1272
Size: 367 905
PstI C_TGCA'G
Cuts at: 0 247 1272
Size: 247 1025
PvuII CAG'CTG
Cuts at: 0 248 1272
Size: 248 1024
SapI GCTCTTCn'nnn_
Cuts at: 0 262 1272
Size: 262 1010
SfcI C'TryA_G
Cuts at: 0 169 243 1272
Size: 169 74 1029
Fragments arranged by size:
1029 169 74
SfoI GGC'GCC
Cuts at: 0 208 1272
Size: 208 1064
SspI AAT'ATT
Cuts at: 0 489 1272
Size: 489 783
StyI C'CwwG_G
Cuts at: 0 188 291 1272
Size: 188 103 981
Fragments arranged by size:
981 188 103
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 625 1272
Size: 625 647
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 864 1272
Size: 864 408
XmnI GAAnn'nnTTC
Cuts at: 0 1167 1272
Size: 1167 105
Enzymes that do cut:
Acc65I AcuI AleI AlfI AloI AlwNI ApoI AvaI
BanI BbeI BbvCI BclI Bme1580I BplI BpmI Bpu10I
BsaI BsaAI BsaHI BseRI BsgI BsmI Bsp1286I BspHI
BspMI BsrBI BsrDI BssHII BstAPI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI EarI
Eco57MI EcoO109I FalI FspI FspAI HaeII KasI KpnI
MscI MslI MspA1I NarI NcoI PflMI PfoI PmlI
PshAI PstI PvuII SapI SfcI SfoI SspI StyI
TaqII XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BanII BbsI BcgI BciVI BfrBI BglI BglII
BlpI BmgBI BmrI BmtI BpuEI BsaBI BsaWI BsaXI
BseYI BsiEI BsiHKAI BsiWI BsmBI BspEI BsrFI BsrGI
BssSI BstBI BstZ17I ClaI CspCI DraI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FseI Hin4I HincII HindIII
HpaI MfeI MluI MmeI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PmeI
PpiI PpuMI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
StuI SwaI TatI TstI Tth111I XbaI XhoI XmaI
ZraI