Restriction map for
pF1KB7024
(Linear) (Six-base) MAPSORT of: KIBB7024.seq Check: 4200 from: 1 to: 1278
KIBB7024.orf
With 182 enzymes: SgfI *
February 20, 2009 16:23 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 328 1278
Size: 295 33 950
Fragments arranged by size:
950 295 33
AleI CACnn'nnGTG
Cuts at: 0 780 1278
Size: 780 498
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 211 1278
Size: 211 1067
AlwNI CAG_nnn'CTG
Cuts at: 0 682 1241 1278
Size: 682 559 37
Fragments arranged by size:
682 559 37
ApaI G_GGCC'C
Cuts at: 0 412 1278
Size: 412 866
AvaI C'yCGr_G
Cuts at: 0 162 411 562 1278
Size: 162 249 151 716
Fragments arranged by size:
716 249 162 151
BanI G'GyrC_C
Cuts at: 0 202 241 259 417 1278
Size: 202 39 18 158 861
Fragments arranged by size:
861 202 158 39 18
BanII G_rGCy'C
Cuts at: 0 412 845 1278
Size: 412 433 433
Fragments arranged by size:
433 433 412
BbeI G_GCGC'C
Cuts at: 0 245 263 421 1278
Size: 245 18 158 857
Fragments arranged by size:
857 245 158 18
BbsI GAAGACnn'nnnn_
Cuts at: 0 814 1278
Size: 814 464
BglI GCCn_nnn'nGGC
Cuts at: 0 250 1278
Size: 250 1028
Bme1580I G_kGCm'C
Cuts at: 0 412 1278
Size: 412 866
BmrI ACTGGGnnnn_n'
Cuts at: 0 807 1278
Size: 807 471
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 319 343 618 778 1038 1278
Size: 60 259 24 275 160 260 240
Fragments arranged by size:
275 260 259 240 160 60 24
Bpu10I CC'TnA_GC
Cuts at: 0 592 1278
Size: 592 686
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 1278
Size: 283 995
BsaHI Gr'CG_yC
Cuts at: 0 33 242 260 418 1278
Size: 33 209 18 158 860
Fragments arranged by size:
860 209 158 33 18
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 33 63 1036 1066 1278
Size: 33 30 973 30 212
Fragments arranged by size:
973 212 33 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 57 85 88 110 113 817 1278
Size: 57 28 3 22 3 704 461
Fragments arranged by size:
704 461 57 28 22 3 3
BseYI C'CCAG_C
Cuts at: 0 405 1205 1278
Size: 405 800 73
Fragments arranged by size:
800 405 73
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 949 1204 1278
Size: 949 255 74
Fragments arranged by size:
949 255 74
BsiEI CG_ry'CG
Cuts at: 0 254 390 1278
Size: 254 136 888
Fragments arranged by size:
888 254 136
BsiHKAI G_wGCw'C
Cuts at: 0 299 1278
Size: 299 979
BsmI GAATG_Cn'
Cuts at: 0 507 1278
Size: 507 771
BsmBI CGTCTCn'nnnn_
Cuts at: 0 40 1278
Size: 40 1238
Bsp1286I G_dGCh'C
Cuts at: 0 299 412 845 1278
Size: 299 113 433 433
Fragments arranged by size:
433 433 299 113
BsrBI CCG'CTC
Cuts at: 0 315 1278
Size: 315 963
BsrFI r'CCGG_y
Cuts at: 0 148 244 424 1278
Size: 148 96 180 854
Fragments arranged by size:
854 180 148 96
BsrGI T'GTAC_A
Cuts at: 0 995 1278
Size: 995 283
BssHII G'CGCG_C
Cuts at: 0 428 1278
Size: 428 850
BssSI C'ACGA_G
Cuts at: 0 1147 1278
Size: 1147 131
BstAPI GCAn_nnn'nTGC
Cuts at: 0 294 371 936 1278
Size: 294 77 565 342
Fragments arranged by size:
565 342 294 77
BstEII G'GTnAC_C
Cuts at: 0 211 1278
Size: 211 1067
BstYI r'GATC_y
Cuts at: 0 270 498 1278
Size: 270 228 780
Fragments arranged by size:
780 270 228
Bsu36I CC'TnA_GG
Cuts at: 0 872 1278
Size: 872 406
BtgI C'CryG_G
Cuts at: 0 236 341 351 1165 1278
Size: 236 105 10 814 113
Fragments arranged by size:
814 236 113 105 10
DraIII CAC_nnn'GTG
Cuts at: 0 294 371 1077 1278
Size: 294 77 706 201
Fragments arranged by size:
706 294 201 77
EaeI y'GGCC_r
Cuts at: 0 251 387 493 1278
Size: 251 136 106 785
Fragments arranged by size:
785 251 136 106
EagI C'GGCC_G
Cuts at: 0 251 387 1278
Size: 251 136 891
Fragments arranged by size:
891 251 136
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 283 1020 1278
Size: 283 737 258
Fragments arranged by size:
737 283 258
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 295 319 328 343 618 778 1038
Size: 60 235 24 9 15 275 160 260
Cuts at: 1038 1278
Size: 240
Fragments arranged by size:
275 260 240 235 160 60 24 15
9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 187 1278
Size: 187 1091
EcoRV GAT'ATC
Cuts at: 0 855 1278
Size: 855 423
FspI TGC'GCA
Cuts at: 0 440 1278
Size: 440 838
HaeII r_GCGC'y
Cuts at: 0 245 263 283 421 1278
Size: 245 18 20 138 857
Fragments arranged by size:
857 245 138 20 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 8 40 1278
Size: 8 32 1238
Fragments arranged by size:
1238 32 8
KasI G'GCGC_C
Cuts at: 0 241 259 417 1278
Size: 241 18 158 861
Fragments arranged by size:
861 241 158 18
MslI CAynn'nnrTG
Cuts at: 0 780 1278
Size: 780 498
MspA1I CmG'CkG
Cuts at: 0 238 321 353 697 924 966 1134 1241
Size: 238 83 32 344 227 42 168 107
Cuts at: 1241 1278
Size: 37
Fragments arranged by size:
344 238 227 168 107 83 42 37
32
NaeI GCC'GGC
Cuts at: 0 246 426 1278
Size: 246 180 852
Fragments arranged by size:
852 246 180
NarI GG'CG_CC
Cuts at: 0 242 260 418 1278
Size: 242 18 158 860
Fragments arranged by size:
860 242 158 18
NgoMIV G'CCGG_C
Cuts at: 0 244 424 1278
Size: 244 180 854
Fragments arranged by size:
854 244 180
PasI CC'CwG_GG
Cuts at: 0 727 1278
Size: 727 551
PflMI CCAn_nnn'nTGG
Cuts at: 0 342 1278
Size: 342 936
PfoI T'CCnGG_A
Cuts at: 0 836 1054 1278
Size: 836 218 224
Fragments arranged by size:
836 224 218
PspOMI G'GGCC_C
Cuts at: 0 408 1278
Size: 408 870
PstI C_TGCA'G
Cuts at: 0 403 669 1133 1278
Size: 403 266 464 145
Fragments arranged by size:
464 403 266 145
PvuII CAG'CTG
Cuts at: 0 321 924 966 1241 1278
Size: 321 603 42 275 37
Fragments arranged by size:
603 321 275 42 37
SacII CC_GC'GG
Cuts at: 0 239 354 1278
Size: 239 115 924
Fragments arranged by size:
924 239 115
SfcI C'TryA_G
Cuts at: 0 399 665 1129 1278
Size: 399 266 464 149
Fragments arranged by size:
464 399 266 149
SfoI GGC'GCC
Cuts at: 0 243 261 419 1278
Size: 243 18 158 859
Fragments arranged by size:
859 243 158 18
SgrAI Cr'CCGG_yG
Cuts at: 0 244 424 1278
Size: 244 180 854
Fragments arranged by size:
854 244 180
SmaI CCC'GGG
Cuts at: 0 164 413 1278
Size: 164 249 865
Fragments arranged by size:
865 249 164
SmlI C'TyrA_G
Cuts at: 0 298 1278
Size: 298 980
SrfI GCCC'GGGC
Cuts at: 0 164 1278
Size: 164 1114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 761 981 1278
Size: 761 220 297
Fragments arranged by size:
761 297 220
TatI w'GTAC_w
Cuts at: 0 995 1278
Size: 995 283
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 33 65 973 1005 1278
Size: 33 32 908 32 273
Fragments arranged by size:
908 273 33 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 642 1062 1278
Size: 642 420 216
Fragments arranged by size:
642 420 216
XmaI C'CCGG_G
Cuts at: 0 162 411 1278
Size: 162 249 867
Fragments arranged by size:
867 249 162
Enzymes that do cut:
AcuI AleI AlfI AlwNI ApaI AvaI BanI BanII
BbeI BbsI BglI Bme1580I BmrI BpmI Bpu10I BpuEI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstEII BstYI Bsu36I BtgI DraIII EaeI EagI EciI
Eco57MI EcoNI EcoRV FspI HaeII Hin4I KasI MslI
MspA1I NaeI NarI NgoMIV PasI PflMI PfoI PspOMI
PstI PvuII SacII SfcI SfoI SgrAI SmaI SmlI
SrfI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI BmgBI BmtI BplI BsaI BsaAI BsaBI
BsaWI BsiWI BspEI BspHI BspMI BsrDI BstBI BstXI
BstZ17I BtgZI BtsI ClaI CspCI DraI DrdI EarI
EcoICRI EcoO109I EcoRI FalI FseI FspAI HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI NcoI NdeI
NheI NotI NruI NsiI NspI PacI PciI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfiI SgfI SnaBI SpeI SphI SspI StuI StyI
SwaI Tth111I XbaI XhoI XmnI ZraI