Restriction map for
pF1KB7021
(Linear) (Six-base) MAPSORT of: KIBB7021.seq Check: 5724 from: 1 to: 1644
KIBB7021.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 347 1644
Size: 347 1297
Acc65I G'GTAC_C
Cuts at: 0 1357 1644
Size: 1357 287
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 799 1644
Size: 799 845
AfeI AGC'GCT
Cuts at: 0 87 1644
Size: 87 1557
AleI CACnn'nnGTG
Cuts at: 0 571 1286 1644
Size: 571 715 358
Fragments arranged by size:
715 571 358
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 861 1644
Size: 861 783
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 232 264 1644
Size: 232 32 1380
Fragments arranged by size:
1380 232 32
AlwNI CAG_nnn'CTG
Cuts at: 0 450 630 684 767 1107 1383 1521 1608
Size: 450 180 54 83 340 276 138 87
Cuts at: 1608 1644
Size: 36
Fragments arranged by size:
450 340 276 180 138 87 83 54
36
ApaI G_GGCC'C
Cuts at: 0 28 1644
Size: 28 1616
ApaLI G'TGCA_C
Cuts at: 0 841 1644
Size: 841 803
AvaI C'yCGr_G
Cuts at: 0 27 1391 1538 1644
Size: 27 1364 147 106
Fragments arranged by size:
1364 147 106 27
BamHI G'GATC_C
Cuts at: 0 924 1644
Size: 924 720
BanI G'GyrC_C
Cuts at: 0 171 576 1054 1076 1118 1191 1257 1357
Size: 171 405 478 22 42 73 66 100
Cuts at: 1357 1644
Size: 287
Fragments arranged by size:
478 405 287 171 100 73 66 42
22
BanII G_rGCy'C
Cuts at: 0 28 713 1399 1644
Size: 28 685 686 245
Fragments arranged by size:
686 685 245 28
BbeI G_GCGC'C
Cuts at: 0 1058 1644
Size: 1058 586
BbvCI CC'TCA_GC
Cuts at: 0 1315 1644
Size: 1315 329
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1519 1553 1644
Size: 1519 34 91
Fragments arranged by size:
1519 91 34
BglII A'GATC_T
Cuts at: 0 1472 1644
Size: 1472 172
BlpI GC'TnA_GC
Cuts at: 0 707 1644
Size: 707 937
Bme1580I G_kGCm'C
Cuts at: 0 28 176 378 845 1644
Size: 28 148 202 467 799
Fragments arranged by size:
799 467 202 148 28
BmrI ACTGGGnnnn_n'
Cuts at: 0 930 1456 1644
Size: 930 526 188
Fragments arranged by size:
930 526 188
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 216 1644
Size: 216 1428
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 708 1156 1644
Size: 708 448 488
Fragments arranged by size:
708 488 448
Bpu10I CC'TnA_GC
Cuts at: 0 1315 1644
Size: 1315 329
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 1644
Size: 388 1256
BsaAI yAC'GTr
Cuts at: 0 879 1644
Size: 879 765
BsaHI Gr'CG_yC
Cuts at: 0 106 1055 1568 1644
Size: 106 949 513 76
Fragments arranged by size:
949 513 106 76
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1526 1644
Size: 1526 118
BseYI C'CCAG_C
Cuts at: 0 121 693 749 825 890 1349 1561 1571
Size: 121 572 56 76 65 459 212 10
Cuts at: 1571 1588 1644
Size: 17 56
Fragments arranged by size:
572 459 212 121 76 65 56 56
17 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 643 753 1093 1396 1549 1644
Size: 643 110 340 303 153 95
Fragments arranged by size:
643 340 303 153 110 95
BsiEI CG_ry'CG
Cuts at: 0 1296 1644
Size: 1296 348
BsiHKAI G_wGCw'C
Cuts at: 0 617 845 1644
Size: 617 228 799
Fragments arranged by size:
799 617 228
BsmI GAATG_Cn'
Cuts at: 0 222 1644
Size: 222 1422
Bsp1286I G_dGCh'C
Cuts at: 0 28 176 378 617 713 845 1399 1644
Size: 28 148 202 239 96 132 554 245
Fragments arranged by size:
554 245 239 202 148 132 96 28
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 191 347 449 506 638 1644
Size: 191 156 102 57 132 1006
Fragments arranged by size:
1006 191 156 132 102 57
BsrBI CCG'CTC
Cuts at: 0 798 1644
Size: 798 846
BsrFI r'CCGG_y
Cuts at: 0 1291 1644
Size: 1291 353
BstAPI GCAn_nnn'nTGC
Cuts at: 0 450 621 630 975 1383 1644
Size: 450 171 9 345 408 261
Fragments arranged by size:
450 408 345 261 171 9
BstEII G'GTnAC_C
Cuts at: 0 1248 1644
Size: 1248 396
BstXI CCAn_nnnn'nTGG
Cuts at: 0 898 1644
Size: 898 746
BstYI r'GATC_y
Cuts at: 0 233 924 1340 1472 1632 1644
Size: 233 691 416 132 160 12
Fragments arranged by size:
691 416 233 160 132 12
BtgI C'CryG_G
Cuts at: 0 569 1031 1162 1644
Size: 569 462 131 482
Fragments arranged by size:
569 482 462 131
BtsI GCAGTG_nn'
Cuts at: 0 448 704 843 845 1105 1458 1644
Size: 448 256 139 2 260 353 186
Fragments arranged by size:
448 353 260 256 186 139 2
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 648 683 1644
Size: 648 35 961
Fragments arranged by size:
961 648 35
DraIII CAC_nnn'GTG
Cuts at: 0 621 1626 1644
Size: 621 1005 18
Fragments arranged by size:
1005 621 18
EaeI y'GGCC_r
Cuts at: 0 428 1293 1428 1644
Size: 428 865 135 216
Fragments arranged by size:
865 428 216 135
EagI C'GGCC_G
Cuts at: 0 1293 1644
Size: 1293 351
EarI CTCTTCn'nnn_
Cuts at: 0 355 1644
Size: 355 1289
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 708 799 1156 1644
Size: 708 91 357 488
Fragments arranged by size:
708 488 357 91
EcoO109I rG'GnC_Cy
Cuts at: 0 24 95 627 1380 1644
Size: 24 71 532 753 264
Fragments arranged by size:
753 532 264 71 24
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 408 1644
Size: 408 1236
FspI TGC'GCA
Cuts at: 0 979 1644
Size: 979 665
HaeII r_GCGC'y
Cuts at: 0 49 89 1058 1644
Size: 49 40 969 586
Fragments arranged by size:
969 586 49 40
KasI G'GCGC_C
Cuts at: 0 1054 1644
Size: 1054 590
KpnI G_GTAC'C
Cuts at: 0 1361 1644
Size: 1361 283
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 314 1644
Size: 314 1330
MscI TGG'CCA
Cuts at: 0 430 1644
Size: 430 1214
MslI CAynn'nnrTG
Cuts at: 0 571 1082 1286 1627 1644
Size: 571 511 204 341 17
Fragments arranged by size:
571 511 341 204 17
MspA1I CmG'CkG
Cuts at: 0 125 264 636 873 894 1073 1644
Size: 125 139 372 237 21 179 571
Fragments arranged by size:
571 372 237 179 139 125 21
NaeI GCC'GGC
Cuts at: 0 1293 1644
Size: 1293 351
NarI GG'CG_CC
Cuts at: 0 1055 1644
Size: 1055 589
NgoMIV G'CCGG_C
Cuts at: 0 1291 1644
Size: 1291 353
NspI r_CATG'y
Cuts at: 0 1557 1644
Size: 1557 87
PasI CC'CwG_GG
Cuts at: 0 1239 1644
Size: 1239 405
PflMI CCAn_nnn'nTGG
Cuts at: 0 684 1521 1644
Size: 684 837 123
Fragments arranged by size:
837 684 123
PfoI T'CCnGG_A
Cuts at: 0 236 608 1514 1644
Size: 236 372 906 130
Fragments arranged by size:
906 372 236 130
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 172 204 1644
Size: 172 32 1440
Fragments arranged by size:
1440 172 32
PpuMI rG'GwC_Cy
Cuts at: 0 627 1644
Size: 627 1017
PshAI GACnn'nnGTC
Cuts at: 0 405 1644
Size: 405 1239
PspOMI G'GGCC_C
Cuts at: 0 24 1644
Size: 24 1620
PstI C_TGCA'G
Cuts at: 0 272 344 446 840 1412 1644
Size: 272 72 102 394 572 232
Fragments arranged by size:
572 394 272 232 102 72
PvuII CAG'CTG
Cuts at: 0 125 873 894 1644
Size: 125 748 21 750
Fragments arranged by size:
750 748 125 21
SfcI C'TryA_G
Cuts at: 0 268 340 442 836 1408 1644
Size: 268 72 102 394 572 236
Fragments arranged by size:
572 394 268 236 102 72
SfoI GGC'GCC
Cuts at: 0 1056 1644
Size: 1056 588
SmaI CCC'GGG
Cuts at: 0 29 1393 1644
Size: 29 1364 251
Fragments arranged by size:
1364 251 29
SmlI C'TyrA_G
Cuts at: 0 403 1644
Size: 403 1241
StyI C'CwwG_G
Cuts at: 0 1046 1644
Size: 1046 598
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 594 1234 1414 1483 1644
Size: 594 640 180 69 161
Fragments arranged by size:
640 594 180 161 69
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 570 1644
Size: 570 1074
XmaI C'CCGG_G
Cuts at: 0 27 1391 1644
Size: 27 1364 253
Fragments arranged by size:
1364 253 27
XmnI GAAnn'nnTTC
Cuts at: 0 1087 1644
Size: 1087 557
Enzymes that do cut:
AarI Acc65I AcuI AfeI AleI AlfI AloI AlwNI
ApaI ApaLI AvaI BamHI BanI BanII BbeI BbvCI
BcgI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaAI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspMI BsrBI BsrFI BstAPI BstEII BstXI
BstYI BtgI BtsI CspCI DraIII EaeI EagI EarI
Eco57MI EcoO109I FalI FspI HaeII KasI KpnI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PflMI PfoI PpiI PpuMI PshAI PspOMI PstI PvuII
SfcI SfoI SmaI SmlI StyI TaqII XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AccI AclI AflII AflIII AgeI AhdI ApoI
AscI AseI AsiSI AvrII BaeI BbsI BciVI BclI
BfrBI BglI BmgBI BmtI BsaI BsaBI BsaWI BsaXI
BsiWI BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI
BstBI BstZ17I Bsu36I BtgZI ClaI DraI DrdI EciI
EcoICRI EcoNI EcoRI EcoRV FseI FspAI Hin4I HincII
HindIII HpaI MfeI MluI NcoI NdeI NheI NotI
NruI NsiI PacI PciI PmeI PmlI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI TstI Tth111I
XbaI XhoI ZraI