Restriction map for
pF1KB7017
(Linear) (Six-base) MAPSORT of: KIBB7017.seq Check: 9063 from: 1 to: 2664
KIBB7017.orf
With 182 enzymes: SgfI *
August 12, 2008 14:28 ..
AatII G_ACGT'C
Cuts at: 0 850 1405 2664
Size: 850 555 1259
Fragments arranged by size:
1259 850 555
AccI GT'mk_AC
Cuts at: 0 722 2664
Size: 722 1942
Acc65I G'GTAC_C
Cuts at: 0 296 2664
Size: 296 2368
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 877 1830 2664
Size: 877 953 834
Fragments arranged by size:
953 877 834
AflIII A'CryG_T
Cuts at: 0 341 1697 2540 2664
Size: 341 1356 843 124
Fragments arranged by size:
1356 843 341 124
AleI CACnn'nnGTG
Cuts at: 0 928 2664
Size: 928 1736
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1172 2664
Size: 1172 1492
AlwNI CAG_nnn'CTG
Cuts at: 0 583 1491 2664
Size: 583 908 1173
Fragments arranged by size:
1173 908 583
ApaI G_GGCC'C
Cuts at: 0 158 1641 2096 2194 2274 2419 2503 2664
Size: 158 1483 455 98 80 145 84 161
Fragments arranged by size:
1483 455 161 158 145 98 84 80
AvaI C'yCGr_G
Cuts at: 0 628 791 940 1072 1123 1141 1283 1587
Size: 628 163 149 132 51 18 142 304
Cuts at: 1587 1668 1984 2109 2128 2267 2275 2664
Size: 81 316 125 19 139 8 389
Fragments arranged by size:
628 389 316 304 163 149 142 139
132 125 81 51 19 18 8
BamHI G'GATC_C
Cuts at: 0 1250 2664
Size: 1250 1414
BanI G'GyrC_C
Cuts at: 0 64 296 1092 1107 1224 1271 1672 2014
Size: 64 232 796 15 117 47 401 342
Cuts at: 2014 2043 2259 2664
Size: 29 216 405
Fragments arranged by size:
796 405 401 342 232 216 117 64
47 29 15
BanII G_rGCy'C
Cuts at: 0 158 275 1412 1641 2096 2194 2274 2419
Size: 158 117 1137 229 455 98 80 145
Cuts at: 2419 2467 2503 2664
Size: 48 36 161
Fragments arranged by size:
1137 455 229 161 158 145 117 98
80 48 36
BbeI G_GCGC'C
Cuts at: 0 68 2018 2047 2263 2664
Size: 68 1950 29 216 401
Fragments arranged by size:
1950 401 216 68 29
BbsI GAAGACnn'nnnn_
Cuts at: 0 1326 1410 2664
Size: 1326 84 1254
Fragments arranged by size:
1326 1254 84
BbvCI CC'TCA_GC
Cuts at: 0 1663 2433 2664
Size: 1663 770 231
Fragments arranged by size:
1663 770 231
BglI GCCn_nnn'nGGC
Cuts at: 0 944 1316 1918 1961 2041 2515 2582 2664
Size: 944 372 602 43 80 474 67 82
Fragments arranged by size:
944 602 474 372 82 80 67 43
BglII A'GATC_T
Cuts at: 0 319 2664
Size: 319 2345
BlpI GC'TnA_GC
Cuts at: 0 100 1495 1945 2664
Size: 100 1395 450 719
Fragments arranged by size:
1395 719 450 100
Bme1580I G_kGCm'C
Cuts at: 0 158 1229 1641 1675 2096 2194 2274 2419
Size: 158 1071 412 34 421 98 80 145
Cuts at: 2419 2503 2605 2663 2664
Size: 84 102 58 1
Fragments arranged by size:
1071 421 412 158 145 102 98 84
80 58 34 1
BmgBI CAC'GTC
Cuts at: 0 281 810 1299 1698 2664
Size: 281 529 489 399 966
Fragments arranged by size:
966 529 489 399 281
BmrI ACTGGGnnnn_n'
Cuts at: 0 67 2506 2664
Size: 67 2439 158
Fragments arranged by size:
2439 158 67
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 336 784 853 1146 1444 2481 2664
Size: 289 47 448 69 293 298 1037 183
Fragments arranged by size:
1037 448 298 293 289 183 69 47
Bpu10I CC'TnA_GC
Cuts at: 0 1663 1722 2433 2664
Size: 1663 59 711 231
Fragments arranged by size:
1663 711 231 59
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1348 2664
Size: 1348 1316
BsaI GGTCTCn'nnnn_
Cuts at: 0 1429 2664
Size: 1429 1235
BsaHI Gr'CG_yC
Cuts at: 0 65 162 847 1087 1402 2015 2044 2137
Size: 65 97 685 240 315 613 29 93
Cuts at: 2137 2260 2664
Size: 123 404
Fragments arranged by size:
685 613 404 315 240 123 97 93
65 29
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 280 310 358 388 826 856 1144 1174
Size: 280 30 48 30 438 30 288 30
Cuts at: 1174 1820 1850 2664
Size: 646 30 814
Fragments arranged by size:
814 646 438 288 280 48 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 100 1441 2502 2611 2664
Size: 100 1341 1061 109 53
Fragments arranged by size:
1341 1061 109 100 53
BseYI C'CCAG_C
Cuts at: 0 328 979 1233 2063 2427 2664
Size: 328 651 254 830 364 237
Fragments arranged by size:
830 651 364 328 254 237
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1716 2664
Size: 1716 948
BsiEI CG_ry'CG
Cuts at: 0 1447 2316 2340 2519 2599 2664
Size: 1447 869 24 179 80 65
Fragments arranged by size:
1447 869 179 80 65 24
BsiWI C'GTAC_G
Cuts at: 0 524 2664
Size: 524 2140
Bsp1286I G_dGCh'C
Cuts at: 0 158 275 1229 1412 1641 1675 2096 2194
Size: 158 117 954 183 229 34 421 98
Cuts at: 2194 2274 2419 2467 2503 2605 2663 2664
Size: 80 145 48 36 102 58 1
Fragments arranged by size:
954 421 229 183 158 145 117 102
98 80 58 48 36 34 1
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1799 2664
Size: 1799 865
BsrBI CCG'CTC
Cuts at: 0 1878 2364 2664
Size: 1878 486 300
Fragments arranged by size:
1878 486 300
BsrFI r'CCGG_y
Cuts at: 0 947 1089 1306 1442 2390 2664
Size: 947 142 217 136 948 274
Fragments arranged by size:
948 947 274 217 142 136
BstAPI GCAn_nnn'nTGC
Cuts at: 0 181 1491 2664
Size: 181 1310 1173
Fragments arranged by size:
1310 1173 181
BstYI r'GATC_y
Cuts at: 0 319 1250 2664
Size: 319 931 1414
Fragments arranged by size:
1414 931 319
Bsu36I CC'TnA_GG
Cuts at: 0 83 1395 1716 2485 2664
Size: 83 1312 321 769 179
Fragments arranged by size:
1312 769 321 179 83
BtgI C'CryG_G
Cuts at: 0 69 284 953 1832 2208 2349 2411 2452
Size: 69 215 669 879 376 141 62 41
Cuts at: 2452 2580 2664
Size: 128 84
Fragments arranged by size:
879 669 376 215 141 128 84 69
62 41
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 760 2664
Size: 760 1904
BtsI GCAGTG_nn'
Cuts at: 0 847 2664
Size: 847 1817
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 405 440 2664
Size: 405 35 2224
Fragments arranged by size:
2224 405 35
EaeI y'GGCC_r
Cuts at: 0 962 1444 2211 2313 2337 2516 2596 2664
Size: 962 482 767 102 24 179 80 68
Fragments arranged by size:
962 767 482 179 102 80 68 24
EagI C'GGCC_G
Cuts at: 0 1444 2313 2337 2516 2596 2664
Size: 1444 869 24 179 80 68
Fragments arranged by size:
1444 869 179 80 68 24
EarI CTCTTCn'nnn_
Cuts at: 0 559 577 2664
Size: 559 18 2087
Fragments arranged by size:
2087 559 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2012 2653 2664
Size: 2012 641 11
Fragments arranged by size:
2012 641 11
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 336 784 853 877 1146 1444 1830
Size: 289 47 448 69 24 269 298 386
Cuts at: 1830 2481 2664
Size: 651 183
Fragments arranged by size:
651 448 386 298 289 269 183 69
47 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 766 1024 1759 2664
Size: 766 258 735 905
Fragments arranged by size:
905 766 735 258
EcoO109I rG'GnC_Cy
Cuts at: 0 1524 1620 1638 1981 2034 2093 2191 2271
Size: 1524 96 18 343 53 59 98 80
Cuts at: 2271 2416 2500 2664
Size: 145 84 164
Fragments arranged by size:
1524 343 164 145 98 96 84 80
59 53 18
FseI GG_CCGG'CC
Cuts at: 0 951 2664
Size: 951 1713
FspI TGC'GCA
Cuts at: 0 649 1134 2664
Size: 649 485 1530
Fragments arranged by size:
1530 649 485
HaeII r_GCGC'y
Cuts at: 0 68 1169 2018 2047 2157 2263 2664
Size: 68 1101 849 29 110 106 401
Fragments arranged by size:
1101 849 401 110 106 68 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 485 517 1051 1083 1191 1223 2092 2124
Size: 485 32 534 32 108 32 869 32
Cuts at: 2124 2416 2448 2664
Size: 292 32 216
Fragments arranged by size:
869 534 485 292 216 108 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 219 2664
Size: 219 2445
KasI G'GCGC_C
Cuts at: 0 64 2014 2043 2259 2664
Size: 64 1950 29 216 405
Fragments arranged by size:
1950 405 216 64 29
KpnI G_GTAC'C
Cuts at: 0 300 2664
Size: 300 2364
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 461 471 2664
Size: 328 133 10 2193
Fragments arranged by size:
2193 328 133 10
MslI CAynn'nnrTG
Cuts at: 0 340 542 928 2664
Size: 340 202 386 1736
Fragments arranged by size:
1736 386 340 202
MspA1I CmG'CkG
Cuts at: 0 152 506 518 1036 1278 1488 1547 2142
Size: 152 354 12 518 242 210 59 595
Cuts at: 2142 2454 2552 2582 2664
Size: 312 98 30 82
Fragments arranged by size:
595 518 354 312 242 210 152 98
82 59 30 12
NaeI GCC'GGC
Cuts at: 0 949 1308 2392 2664
Size: 949 359 1084 272
Fragments arranged by size:
1084 949 359 272
NarI GG'CG_CC
Cuts at: 0 65 2015 2044 2260 2664
Size: 65 1950 29 216 404
Fragments arranged by size:
1950 404 216 65 29
NgoMIV G'CCGG_C
Cuts at: 0 947 1306 2390 2664
Size: 947 359 1084 274
Fragments arranged by size:
1084 947 359 274
PasI CC'CwG_GG
Cuts at: 0 113 1313 2187 2664
Size: 113 1200 874 477
Fragments arranged by size:
1200 874 477 113
PflMI CCAn_nnn'nTGG
Cuts at: 0 702 2103 2187 2664
Size: 702 1401 84 477
Fragments arranged by size:
1401 702 477 84
PfoI T'CCnGG_A
Cuts at: 0 734 1421 2664
Size: 734 687 1243
Fragments arranged by size:
1243 734 687
PpuMI rG'GwC_Cy
Cuts at: 0 1620 1981 2664
Size: 1620 361 683
Fragments arranged by size:
1620 683 361
PshAI GACnn'nnGTC
Cuts at: 0 1330 2664
Size: 1330 1334
PspOMI G'GGCC_C
Cuts at: 0 154 1637 2092 2190 2270 2415 2499 2664
Size: 154 1483 455 98 80 145 84 165
Fragments arranged by size:
1483 455 165 154 145 98 84 80
PspXI vC'TCGA_Gb
Cuts at: 0 1283 2664
Size: 1283 1381
PstI C_TGCA'G
Cuts at: 0 485 917 2087 2664
Size: 485 432 1170 577
Fragments arranged by size:
1170 577 485 432
PvuII CAG'CTG
Cuts at: 0 1036 1278 2664
Size: 1036 242 1386
Fragments arranged by size:
1386 1036 242
RsrII CG'GwC_CG
Cuts at: 0 2385 2664
Size: 2385 279
SacII CC_GC'GG
Cuts at: 0 2455 2583 2664
Size: 2455 128 81
Fragments arranged by size:
2455 128 81
SanDI GG'GwC_CC
Cuts at: 0 1981 2664
Size: 1981 683
SapI GCTCTTCn'nnn_
Cuts at: 0 577 2664
Size: 577 2087
ScaI AGT'ACT
Cuts at: 0 1585 2664
Size: 1585 1079
SfcI C'TryA_G
Cuts at: 0 465 481 913 2083 2664
Size: 465 16 432 1170 581
Fragments arranged by size:
1170 581 465 432 16
SfoI GGC'GCC
Cuts at: 0 66 2016 2045 2261 2664
Size: 66 1950 29 216 403
Fragments arranged by size:
1950 403 216 66 29
SgrAI Cr'CCGG_yG
Cuts at: 0 1089 2390 2664
Size: 1089 1301 274
Fragments arranged by size:
1301 1089 274
SmaI CCC'GGG
Cuts at: 0 1125 1143 1670 1986 2269 2664
Size: 1125 18 527 316 283 395
Fragments arranged by size:
1125 527 395 316 283 18
SmlI C'TyrA_G
Cuts at: 0 1283 1363 2664
Size: 1283 80 1301
Fragments arranged by size:
1301 1283 80
SrfI GCCC'GGGC
Cuts at: 0 1670 2269 2664
Size: 1670 599 395
Fragments arranged by size:
1670 599 395
StyI C'CwwG_G
Cuts at: 0 2069 2420 2664
Size: 2069 351 244
Fragments arranged by size:
2069 351 244
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2551 2664
Size: 2551 113
TatI w'GTAC_w
Cuts at: 0 1151 1583 2664
Size: 1151 432 1081
Fragments arranged by size:
1151 1081 432
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 358 390 826 858 1820 1852 2664
Size: 358 32 436 32 962 32 812
Fragments arranged by size:
962 812 436 358 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2418 2664
Size: 2418 246
XhoI C'TCGA_G
Cuts at: 0 1283 2664
Size: 1283 1381
XmaI C'CCGG_G
Cuts at: 0 1123 1141 1668 1984 2267 2664
Size: 1123 18 527 316 283 397
Fragments arranged by size:
1123 527 397 316 283 18
ZraI GAC'GTC
Cuts at: 0 848 1403 2664
Size: 848 555 1261
Fragments arranged by size:
1261 848 555
Enzymes that do cut:
AatII AccI Acc65I AcuI AflIII AleI AlfI AlwNI
ApaI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BglI BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I
BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiWI Bsp1286I BspMI BsrBI BsrFI BstAPI BstYI Bsu36I
BtgI BtgZI BtsI CspCI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I FseI FspI HaeII Hin4I HincII
KasI KpnI MmeI MslI MspA1I NaeI NarI NgoMIV
PasI PflMI PfoI PpuMI PshAI PspOMI PspXI PstI
PvuII RsrII SacII SanDI SapI ScaI SfcI SfoI
SgrAI SmaI SmlI SrfI StyI TaqII TatI TstI
XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AarI AclI AfeI AflII AgeI AhdI AloI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BcgI BciVI
BclI BfrBI BmtI BplI BsaAI BsaBI BsaWI BsiHKAI
BsmI BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI
BstBI BstEII BstXI BstZ17I ClaI DraI DraIII DrdI
EcoICRI EcoRI EcoRV FalI FspAI HindIII HpaI MfeI
MluI MscI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PciI PmeI PmlI PpiI PsiI PsrI
PvuI SacI SalI SbfI SexAI SfiI SgfI SnaBI
SpeI SphI SspI StuI SwaI Tth111I XbaI XmnI