Restriction map for
pF1KB7013
(Linear) (Six-base) MAPSORT of: KIBB7013.seq Check: 8674 from: 1 to: 3258
KIBB7013.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 316 1705 3099 3258
Size: 316 1389 1394 159
Fragments arranged by size:
1394 1389 316 159
AccI GT'mk_AC
Cuts at: 0 75 932 3258
Size: 75 857 2326
Fragments arranged by size:
2326 857 75
Acc65I G'GTAC_C
Cuts at: 0 2050 3258
Size: 2050 1208
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1207 1389 3258
Size: 1207 182 1869
Fragments arranged by size:
1869 1207 182
AflIII A'CryG_T
Cuts at: 0 78 1748 3214 3258
Size: 78 1670 1466 44
Fragments arranged by size:
1670 1466 78 44
AlwNI CAG_nnn'CTG
Cuts at: 0 664 1366 2434 2998 3258
Size: 664 702 1068 564 260
Fragments arranged by size:
1068 702 664 564 260
ApaI G_GGCC'C
Cuts at: 0 2008 2535 3258
Size: 2008 527 723
Fragments arranged by size:
2008 723 527
ApaLI G'TGCA_C
Cuts at: 0 2585 3258
Size: 2585 673
ApoI r'AATT_y
Cuts at: 0 152 2711 2882 3258
Size: 152 2559 171 376
Fragments arranged by size:
2559 376 171 152
AvaI C'yCGr_G
Cuts at: 0 1878 3258
Size: 1878 1380
AvrII C'CTAG_G
Cuts at: 0 1872 3258
Size: 1872 1386
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1633 1666 3258
Size: 1633 33 1592
Fragments arranged by size:
1633 1592 33
BanI G'GyrC_C
Cuts at: 0 328 1339 1807 1897 2050 2677 3258
Size: 328 1011 468 90 153 627 581
Fragments arranged by size:
1011 627 581 468 328 153 90
BanII G_rGCy'C
Cuts at: 0 390 818 2008 2535 3258
Size: 390 428 1190 527 723
Fragments arranged by size:
1190 723 527 428 390
BbsI GAAGACnn'nnnn_
Cuts at: 0 1363 1417 3258
Size: 1363 54 1841
Fragments arranged by size:
1841 1363 54
BbvCI CC'TCA_GC
Cuts at: 0 1043 2933 3258
Size: 1043 1890 325
Fragments arranged by size:
1890 1043 325
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 722 756 3258
Size: 722 34 2502
Fragments arranged by size:
2502 722 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1448 1661 3258
Size: 1448 213 1597
Fragments arranged by size:
1597 1448 213
BclI T'GATC_A
Cuts at: 0 309 3258
Size: 309 2949
BglI GCCn_nnn'nGGC
Cuts at: 0 1147 2328 3258
Size: 1147 1181 930
Fragments arranged by size:
1181 1147 930
BlpI GC'TnA_GC
Cuts at: 0 574 812 1367 3258
Size: 574 238 555 1891
Fragments arranged by size:
1891 574 555 238
Bme1580I G_kGCm'C
Cuts at: 0 333 1344 2008 2535 2589 3258
Size: 333 1011 664 527 54 669
Fragments arranged by size:
1011 669 664 527 333 54
BmgBI CAC'GTC
Cuts at: 0 1933 3258
Size: 1933 1325
BmrI ACTGGGnnnn_n'
Cuts at: 0 1339 1468 1957 2884 3258
Size: 1339 129 489 927 374
Fragments arranged by size:
1339 927 489 374 129
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 662 959 1988 2051 3211 3258
Size: 662 297 1029 63 1160 47
Fragments arranged by size:
1160 1029 662 297 63 47
Bpu10I CC'TnA_GC
Cuts at: 0 557 1043 2933 3258
Size: 557 486 1890 325
Fragments arranged by size:
1890 557 486 325
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1319 3032 3109 3258
Size: 1319 1713 77 149
Fragments arranged by size:
1713 1319 149 77
BsaI GGTCTCn'nnnn_
Cuts at: 0 1450 2270 2299 2806 3258
Size: 1450 820 29 507 452
Fragments arranged by size:
1450 820 507 452 29
BsaAI yAC'GTr
Cuts at: 0 79 3258
Size: 79 3179
BsaHI Gr'CG_yC
Cuts at: 0 2249 3258
Size: 2249 1009
BseYI C'CCAG_C
Cuts at: 0 468 712 1129 1308 2528 3258
Size: 468 244 417 179 1220 730
Fragments arranged by size:
1220 730 468 417 244 179
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2561 2828 3258
Size: 2561 267 430
Fragments arranged by size:
2561 430 267
BsiHKAI G_wGCw'C
Cuts at: 0 2589 3258
Size: 2589 669
BsmI GAATG_Cn'
Cuts at: 0 1690 1745 2909 3258
Size: 1690 55 1164 349
Fragments arranged by size:
1690 1164 349 55
Bsp1286I G_dGCh'C
Cuts at: 0 333 390 818 1344 2008 2535 2589 3258
Size: 333 57 428 526 664 527 54 669
Fragments arranged by size:
669 664 527 526 428 333 57 54
BspHI T'CATG_A
Cuts at: 0 417 3258
Size: 417 2841
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 316 1133 1432 1705 1802 3099 3258
Size: 316 817 299 273 97 1297 159
Fragments arranged by size:
1297 817 316 299 273 159 97
BsrDI GCAATG_nn'
Cuts at: 0 533 590 1469 1709 1947 2270 2642 2828
Size: 533 57 879 240 238 323 372 186
Cuts at: 2828 3258
Size: 430
Fragments arranged by size:
879 533 430 372 323 240 238 186
57
BsrFI r'CCGG_y
Cuts at: 0 1804 3258
Size: 1804 1454
BssSI C'ACGA_G
Cuts at: 0 36 3258
Size: 36 3222
BstEII G'GTnAC_C
Cuts at: 0 1503 3258
Size: 1503 1755
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1137 2220 3258
Size: 1137 1083 1038
Fragments arranged by size:
1137 1083 1038
BstYI r'GATC_y
Cuts at: 0 3054 3258
Size: 3054 204
BtgI C'CryG_G
Cuts at: 0 581 2705 3258
Size: 581 2124 553
Fragments arranged by size:
2124 581 553
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2050 3258
Size: 2050 1208
BtsI GCAGTG_nn'
Cuts at: 0 3158 3258
Size: 3158 100
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 106 141 3258
Size: 106 35 3117
Fragments arranged by size:
3117 106 35
DraI TTT'AAA
Cuts at: 0 434 3258
Size: 434 2824
DrdI GACnn_nn'nnGTC
Cuts at: 0 2494 3258
Size: 2494 764
EaeI y'GGCC_r
Cuts at: 0 584 1139 3185 3258
Size: 584 555 2046 73
Fragments arranged by size:
2046 584 555 73
EarI CTCTTCn'nnn_
Cuts at: 0 3118 3258
Size: 3118 140
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 662 959 1207 1389 1988 2051 3211 3258
Size: 662 297 248 182 599 63 1160 47
Fragments arranged by size:
1160 662 599 297 248 182 63 47
EcoO109I rG'GnC_Cy
Cuts at: 0 62 1671 2532 3258
Size: 62 1609 861 726
Fragments arranged by size:
1609 861 726 62
EcoRI G'AATT_C
Cuts at: 0 152 2882 3258
Size: 152 2730 376
Fragments arranged by size:
2730 376 152
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 445 3258
Size: 445 2813
HaeII r_GCGC'y
Cuts at: 0 139 3258
Size: 139 3119
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1548 1580 3046 3078 3258
Size: 1548 32 1466 32 180
Fragments arranged by size:
1548 1466 180 32 32
HincII GTy'rAC
Cuts at: 0 1421 1707 3258
Size: 1421 286 1551
Fragments arranged by size:
1551 1421 286
HindIII A'AGCT_T
Cuts at: 0 290 2397 3258
Size: 290 2107 861
Fragments arranged by size:
2107 861 290
KpnI G_GTAC'C
Cuts at: 0 2054 3258
Size: 2054 1204
MfeI C'AATT_G
Cuts at: 0 551 752 2066 2750 3258
Size: 551 201 1314 684 508
Fragments arranged by size:
1314 684 551 508 201
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 156 427 1190 2942 3258
Size: 156 271 763 1752 316
Fragments arranged by size:
1752 763 316 271 156
MscI TGG'CCA
Cuts at: 0 586 1141 3258
Size: 586 555 2117
Fragments arranged by size:
2117 586 555
MslI CAynn'nnrTG
Cuts at: 0 2455 2629 3187 3258
Size: 2455 174 558 71
Fragments arranged by size:
2455 558 174 71
MspA1I CmG'CkG
Cuts at: 0 661 2140 2470 2692 3258
Size: 661 1479 330 222 566
Fragments arranged by size:
1479 661 566 330 222
NaeI GCC'GGC
Cuts at: 0 1806 3258
Size: 1806 1452
NcoI C'CATG_G
Cuts at: 0 581 2705 3258
Size: 581 2124 553
Fragments arranged by size:
2124 581 553
NdeI CA'TA_TG
Cuts at: 0 2949 3258
Size: 2949 309
NgoMIV G'CCGG_C
Cuts at: 0 1804 3258
Size: 1804 1454
NspI r_CATG'y
Cuts at: 0 1752 2493 3081 3218 3258
Size: 1752 741 588 137 40
Fragments arranged by size:
1752 741 588 137 40
PasI CC'CwG_GG
Cuts at: 0 1920 3258
Size: 1920 1338
PciI A'CATG_T
Cuts at: 0 1748 3214 3258
Size: 1748 1466 44
Fragments arranged by size:
1748 1466 44
PflMI CCAn_nnn'nTGG
Cuts at: 0 2797 3258
Size: 2797 461
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2506 2538 3258
Size: 2506 32 720
Fragments arranged by size:
2506 720 32
PpuMI rG'GwC_Cy
Cuts at: 0 62 3258
Size: 62 3196
PsiI TTA'TAA
Cuts at: 0 602 1850 3258
Size: 602 1248 1408
Fragments arranged by size:
1408 1248 602
PspOMI G'GGCC_C
Cuts at: 0 2004 2531 3258
Size: 2004 527 727
Fragments arranged by size:
2004 727 527
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 93 125 3258
Size: 93 32 3133
Fragments arranged by size:
3133 93 32
PstI C_TGCA'G
Cuts at: 0 729 783 1617 3153 3258
Size: 729 54 834 1536 105
Fragments arranged by size:
1536 834 729 105 54
PvuII CAG'CTG
Cuts at: 0 661 2140 2470 2692 3258
Size: 661 1479 330 222 566
Fragments arranged by size:
1479 661 566 330 222
SexAI A'CCwGG_T
Cuts at: 0 533 3258
Size: 533 2725
SfcI C'TryA_G
Cuts at: 0 315 515 725 779 1277 1613 1839 3149
Size: 315 200 210 54 498 336 226 1310
Cuts at: 3149 3258
Size: 109
Fragments arranged by size:
1310 498 336 315 226 210 200 109
54
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1147 3258
Size: 1147 2111
SmlI C'TyrA_G
Cuts at: 0 1334 3047 3088 3258
Size: 1334 1713 41 170
Fragments arranged by size:
1713 1334 170 41
SphI G_CATG'C
Cuts at: 0 3081 3258
Size: 3081 177
SspI AAT'ATT
Cuts at: 0 1329 3258
Size: 1329 1929
StyI C'CwwG_G
Cuts at: 0 339 581 818 1872 1901 2705 3258
Size: 339 242 237 1054 29 804 553
Fragments arranged by size:
1054 804 553 339 242 237 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 736 1933 2035 2443 2572 2903 3124 3258
Size: 736 1197 102 408 129 331 221 134
Fragments arranged by size:
1197 736 408 331 221 134 129 102
TatI w'GTAC_w
Cuts at: 0 2142 2162 3258
Size: 2142 20 1096
Fragments arranged by size:
2142 1096 20
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1265 1297 3258
Size: 1265 32 1961
Fragments arranged by size:
1961 1265 32
Tth111I GACn'n_nGTC
Cuts at: 0 1416 3258
Size: 1416 1842
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1515 2835 3258
Size: 1515 1320 423
Fragments arranged by size:
1515 1320 423
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AlwNI ApaI ApaLI
ApoI AvaI AvrII BaeI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BlpI Bme1580I BmgBI BmrI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrFI BssSI
BstEII BstXI BstYI BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EarI Eco57MI EcoO109I EcoRI FalI HaeII
Hin4I HincII HindIII KpnI MfeI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NspI PasI PciI
PflMI PpiI PpuMI PsiI PspOMI PsrI PstI PvuII
SexAI SfcI SfiI SmlI SphI SspI StyI TaqII
TatI TstI Tth111I XcmI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AhdI AleI AlfI
AloI AscI AseI AsiSI BamHI BbeI BfrBI BglII
BmtI BplI BsaBI BsaWI BsaXI BseRI BsiEI BsiWI
BsmBI BspEI BsrBI BsrGI BssHII BstAPI BstBI BstZ17I
Bsu36I ClaI DraIII EagI EciI EcoICRI EcoNI EcoRV
FseI FspI FspAI HpaI KasI MluI NarI NheI
NotI NruI NsiI PacI PfoI PmeI PmlI PshAI
PspXI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI StuI SwaI XbaI XhoI XmaI XmnI ZraI