Restriction map for
pF1KB7008
(Linear) (Six-base) MAPSORT of: KIBB7008.seq Check: 514 from: 1 to: 1395
KIBB7008.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 546 1029 1166 1395
Size: 546 483 137 229
Fragments arranged by size:
546 483 229 137
AatII G_ACGT'C
Cuts at: 0 332 1395
Size: 332 1063
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 205 1395
Size: 88 117 1190
Fragments arranged by size:
1190 117 88
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 737 1395
Size: 737 658
AlwNI CAG_nnn'CTG
Cuts at: 0 630 1395
Size: 630 765
ApaLI G'TGCA_C
Cuts at: 0 369 1395
Size: 369 1026
AvaI C'yCGr_G
Cuts at: 0 42 170 1395
Size: 42 128 1225
Fragments arranged by size:
1225 128 42
BanI G'GyrC_C
Cuts at: 0 394 487 852 1395
Size: 394 93 365 543
Fragments arranged by size:
543 394 365 93
BanII G_rGCy'C
Cuts at: 0 350 931 1395
Size: 350 581 464
Fragments arranged by size:
581 464 350
BbsI GAAGACnn'nnnn_
Cuts at: 0 220 284 1395
Size: 220 64 1111
Fragments arranged by size:
1111 220 64
BbvCI CC'TCA_GC
Cuts at: 0 437 676 1395
Size: 437 239 719
Fragments arranged by size:
719 437 239
BciVI GTATCCnnnnn_n'
Cuts at: 0 905 1395
Size: 905 490
BglI GCCn_nnn'nGGC
Cuts at: 0 187 448 694 820 870 1133 1315 1395
Size: 187 261 246 126 50 263 182 80
Fragments arranged by size:
263 261 246 187 182 126 80 50
Bme1580I G_kGCm'C
Cuts at: 0 171 373 857 1302 1395
Size: 171 202 484 445 93
Fragments arranged by size:
484 445 202 171 93
BmgBI CAC'GTC
Cuts at: 0 1215 1395
Size: 1215 180
BmrI ACTGGGnnnn_n'
Cuts at: 0 561 878 1395
Size: 561 317 517
Fragments arranged by size:
561 517 317
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 580 1022 1174 1395
Size: 73 507 442 152 221
Fragments arranged by size:
507 442 221 152 73
Bpu10I CC'TnA_GC
Cuts at: 0 437 676 1395
Size: 437 239 719
Fragments arranged by size:
719 437 239
BsaI GGTCTCn'nnnn_
Cuts at: 0 544 1395
Size: 544 851
BsaHI Gr'CG_yC
Cuts at: 0 329 833 1395
Size: 329 504 562
Fragments arranged by size:
562 504 329
BsaWI w'CCGG_w
Cuts at: 0 281 332 1395
Size: 281 51 1063
Fragments arranged by size:
1063 281 51
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 70 287 1395
Size: 70 217 1108
Fragments arranged by size:
1108 217 70
BseYI C'CCAG_C
Cuts at: 0 860 918 996 1052 1395
Size: 860 58 78 56 343
Fragments arranged by size:
860 343 78 58 56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 187 724 1395
Size: 121 66 537 671
Fragments arranged by size:
671 537 121 66
BsiHKAI G_wGCw'C
Cuts at: 0 373 380 1395
Size: 373 7 1015
Fragments arranged by size:
1015 373 7
Bsp1286I G_dGCh'C
Cuts at: 0 171 350 373 380 857 931 1302 1395
Size: 171 179 23 7 477 74 371 93
Fragments arranged by size:
477 371 179 171 93 74 23 7
BspEI T'CCGG_A
Cuts at: 0 332 1395
Size: 332 1063
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 546 1029 1166 1395
Size: 546 483 137 229
Fragments arranged by size:
546 483 229 137
BsrFI r'CCGG_y
Cuts at: 0 1385 1395
Size: 1385 10
BstAPI GCAn_nnn'nTGC
Cuts at: 0 407 1395
Size: 407 988
BstBI TT'CG_AA
Cuts at: 0 80 1395
Size: 80 1315
BstXI CCAn_nnnn'nTGG
Cuts at: 0 504 570 868 1395
Size: 504 66 298 527
Fragments arranged by size:
527 504 298 66
BstYI r'GATC_y
Cuts at: 0 38 95 1184 1395
Size: 38 57 1089 211
Fragments arranged by size:
1089 211 57 38
Bsu36I CC'TnA_GG
Cuts at: 0 768 1373 1395
Size: 768 605 22
Fragments arranged by size:
768 605 22
BtgI C'CryG_G
Cuts at: 0 683 692 932 941 1395
Size: 683 9 240 9 454
Fragments arranged by size:
683 454 240 9 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 573 729 777 1395
Size: 573 156 48 618
Fragments arranged by size:
618 573 156 48
EaeI y'GGCC_r
Cuts at: 0 449 560 686 1209 1292 1395
Size: 449 111 126 523 83 103
Fragments arranged by size:
523 449 126 111 103 83
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 532 1395
Size: 532 863
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 88 205 580 1022 1174 1395
Size: 73 15 117 375 442 152 221
Fragments arranged by size:
442 375 221 152 117 73 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 955 1162 1395
Size: 955 207 233
Fragments arranged by size:
955 233 207
EcoO109I rG'GnC_Cy
Cuts at: 0 63 627 1126 1395
Size: 63 564 499 269
Fragments arranged by size:
564 499 269 63
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 93 1395
Size: 93 1302
HaeII r_GCGC'y
Cuts at: 0 200 617 1350 1395
Size: 200 417 733 45
Fragments arranged by size:
733 417 200 45
HincII GTy'rAC
Cuts at: 0 837 1239 1395
Size: 837 402 156
Fragments arranged by size:
837 402 156
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 1395
Size: 73 1322
MscI TGG'CCA
Cuts at: 0 562 688 1211 1395
Size: 562 126 523 184
Fragments arranged by size:
562 523 184 126
MspA1I CmG'CkG
Cuts at: 0 934 1395
Size: 934 461
NcoI C'CATG_G
Cuts at: 0 683 692 1395
Size: 683 9 703
Fragments arranged by size:
703 683 9
NspI r_CATG'y
Cuts at: 0 552 579 1395
Size: 552 27 816
Fragments arranged by size:
816 552 27
PasI CC'CwG_GG
Cuts at: 0 828 1395
Size: 828 567
PpuMI rG'GwC_Cy
Cuts at: 0 63 1395
Size: 63 1332
PstI C_TGCA'G
Cuts at: 0 956 1395
Size: 956 439
SacII CC_GC'GG
Cuts at: 0 935 1395
Size: 935 460
SfcI C'TryA_G
Cuts at: 0 609 843 952 1005 1395
Size: 609 234 109 53 390
Fragments arranged by size:
609 390 234 109 53
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 448 1395
Size: 448 947
SmaI CCC'GGG
Cuts at: 0 172 1395
Size: 172 1223
SphI G_CATG'C
Cuts at: 0 552 1395
Size: 552 843
StuI AGG'CCT
Cuts at: 0 961 1395
Size: 961 434
StyI C'CwwG_G
Cuts at: 0 683 692 1395
Size: 683 9 703
Fragments arranged by size:
703 683 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 580 637 874 1256 1395
Size: 580 57 237 382 139
Fragments arranged by size:
580 382 237 139 57
TatI w'GTAC_w
Cuts at: 0 125 1395
Size: 125 1270
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1010 1042 1395
Size: 1010 32 353
Fragments arranged by size:
1010 353 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 690 1321 1395
Size: 690 631 74
Fragments arranged by size:
690 631 74
XmaI C'CCGG_G
Cuts at: 0 170 1395
Size: 170 1225
XmnI GAAnn'nnTTC
Cuts at: 0 1327 1395
Size: 1327 68
ZraI GAC'GTC
Cuts at: 0 330 1395
Size: 330 1065
Enzymes that do cut:
AarI AatII AcuI AlfI AlwNI ApaLI AvaI BanI
BanII BbsI BbvCI BciVI BglI Bme1580I BmgBI BmrI
BpmI Bpu10I BsaI BsaHI BsaWI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspEI BspMI BsrFI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI EaeI EciI Eco57MI EcoNI
EcoO109I FalI HaeII HincII MmeI MscI MspA1I NcoI
NspI PasI PpuMI PstI SacII SfcI SfiI SmaI
SphI StuI StyI TaqII TatI TstI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AloI ApaI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BcgI BclI BfrBI BglII BlpI
BmtI BplI BpuEI BsaAI BsaBI BsaXI BsiEI BsiWI
BsmI BsmBI BspHI BsrBI BsrDI BsrGI BssHII BssSI
BstEII BstZ17I BtsI ClaI CspCI DraI DraIII DrdI
EagI EarI EcoICRI EcoRI EcoRV FseI FspI FspAI
Hin4I HindIII HpaI KasI KpnI MfeI MluI MslI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfoI SgfI
SgrAI SmlI SnaBI SpeI SrfI SspI SwaI Tth111I
XbaI XhoI