Restriction map for
pF1KB7004
(Linear) (Six-base) MAPSORT of: KIBB7004.seq Check: 4904 from: 1 to: 4020
KIBB7004.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 288 4020
Size: 288 3732
Acc65I G'GTAC_C
Cuts at: 0 3129 4020
Size: 3129 891
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 734 1682 2386 2584 4020
Size: 734 948 704 198 1436
Fragments arranged by size:
1436 948 734 704 198
AfeI AGC'GCT
Cuts at: 0 859 4020
Size: 859 3161
AflIII A'CryG_T
Cuts at: 0 311 4020
Size: 311 3709
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2356 3333 4020
Size: 2356 977 687
Fragments arranged by size:
2356 977 687
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2413 2445 4020
Size: 2413 32 1575
Fragments arranged by size:
2413 1575 32
AlwNI CAG_nnn'CTG
Cuts at: 0 349 1683 2479 3486 3799 4020
Size: 349 1334 796 1007 313 221
Fragments arranged by size:
1334 1007 796 349 313 221
ApaI G_GGCC'C
Cuts at: 0 692 1043 1831 1885 2008 2119 2195 2473
Size: 692 351 788 54 123 111 76 278
Cuts at: 2473 4020
Size: 1547
Fragments arranged by size:
1547 788 692 351 278 123 111 76
54
ApoI r'AATT_y
Cuts at: 0 133 516 913 1587 1865 4020
Size: 133 383 397 674 278 2155
Fragments arranged by size:
2155 674 397 383 278 133
AvaI C'yCGr_G
Cuts at: 0 1177 1298 1413 2415 3334 3500 3947 4020
Size: 1177 121 115 1002 919 166 447 73
Fragments arranged by size:
1177 1002 919 447 166 121 115 73
AvrII C'CTAG_G
Cuts at: 0 1023 4020
Size: 1023 2997
BamHI G'GATC_C
Cuts at: 0 1957 2612 4020
Size: 1957 655 1408
Fragments arranged by size:
1957 1408 655
BanI G'GyrC_C
Cuts at: 0 561 1356 2070 3129 4020
Size: 561 795 714 1059 891
Fragments arranged by size:
1059 891 795 714 561
BanII G_rGCy'C
Cuts at: 0 31 275 692 800 1043 1062 1831 1885
Size: 31 244 417 108 243 19 769 54
Cuts at: 1885 2008 2119 2195 2410 2473 3845 3871 4020
Size: 123 111 76 215 63 1372 26 149
Fragments arranged by size:
1372 769 417 244 243 215 149 123
111 108 76 63 54 31 26 19
BbsI GAAGACnn'nnnn_
Cuts at: 0 227 295 2728 4020
Size: 227 68 2433 1292
Fragments arranged by size:
2433 1292 227 68
BbvCI CC'TCA_GC
Cuts at: 0 2971 4020
Size: 2971 1049
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1279 1313 4020
Size: 1279 34 2707
Fragments arranged by size:
2707 1279 34
BfrBI ATG'CAT
Cuts at: 0 3 4020
Size: 3 4017
BglI GCCn_nnn'nGGC
Cuts at: 0 1006 2036 3272 3840 4020
Size: 1006 1030 1236 568 180
Fragments arranged by size:
1236 1030 1006 568 180
BglII A'GATC_T
Cuts at: 0 3396 4020
Size: 3396 624
BlpI GC'TnA_GC
Cuts at: 0 2057 4020
Size: 2057 1963
Bme1580I G_kGCm'C
Cuts at: 0 692 1043 1359 1831 1885 2008 2119 2195
Size: 692 351 316 472 54 123 111 76
Cuts at: 2195 2473 4020
Size: 278 1547
Fragments arranged by size:
1547 692 472 351 316 278 123 111
76 54
BmgBI CAC'GTC
Cuts at: 0 592 1053 3966 4020
Size: 592 461 2913 54
Fragments arranged by size:
2913 592 461 54
BmrI ACTGGGnnnn_n'
Cuts at: 0 695 817 1128 2828 3209 4020
Size: 695 122 311 1700 381 811
Fragments arranged by size:
1700 811 695 381 311 122
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 47 4020
Size: 47 3973
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 278 865 1085 1252 1562 2267 2750 2882
Size: 278 587 220 167 310 705 483 132
Cuts at: 2882 3152 3347 3440 3857 4020
Size: 270 195 93 417 163
Fragments arranged by size:
705 587 483 417 310 278 270 220
195 167 163 132 93
Bpu10I CC'TnA_GC
Cuts at: 0 2971 4020
Size: 2971 1049
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1058 1918 2410 2524 3119 4020
Size: 1058 860 492 114 595 901
Fragments arranged by size:
1058 901 860 595 492 114
BsaI GGTCTCn'nnnn_
Cuts at: 0 1708 3684 4020
Size: 1708 1976 336
Fragments arranged by size:
1976 1708 336
BsaAI yAC'GTr
Cuts at: 0 312 4020
Size: 312 3708
BsaBI GATnn'nnATC
Cuts at: 0 1937 4020
Size: 1937 2083
BsaWI w'CCGG_w
Cuts at: 0 3427 3639 4020
Size: 3427 212 381
Fragments arranged by size:
3427 381 212
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2265 2295 2568 2598 2748 2778 4020
Size: 2265 30 273 30 150 30 1242
Fragments arranged by size:
2265 1242 273 150 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 39 1242 2026 2270 3041 4020
Size: 39 1203 784 244 771 979
Fragments arranged by size:
1203 979 784 771 244 39
BseYI C'CCAG_C
Cuts at: 0 119 185 492 1352 1698 1738 1772 1996
Size: 119 66 307 860 346 40 34 224
Cuts at: 1996 2096 2403 2472 2652 2661 2990 3280 3475
Size: 100 307 69 180 9 329 290 195
Cuts at: 3475 4020
Size: 545
Fragments arranged by size:
860 545 346 329 307 307 290 224
195 180 119 100 69 66 40 34
9
BsiHKAI G_wGCw'C
Cuts at: 0 31 851 927 1062 2093 4020
Size: 31 820 76 135 1031 1927
Fragments arranged by size:
1927 1031 820 135 76 31
BsmI GAATG_Cn'
Cuts at: 0 3 2343 4020
Size: 3 2340 1677
Fragments arranged by size:
2340 1677 3
BsmBI CGTCTCn'nnnn_
Cuts at: 0 384 4020
Size: 384 3636
Bsp1286I G_dGCh'C
Cuts at: 0 31 275 692 800 851 927 1043 1062
Size: 31 244 417 108 51 76 116 19
Cuts at: 1062 1359 1831 1885 2008 2093 2119 2195 2410
Size: 297 472 54 123 85 26 76 215
Cuts at: 2410 2473 3845 3871 4020
Size: 63 1372 26 149
Fragments arranged by size:
1372 472 417 297 244 215 149 123
116 108 85 76 76 63 54 51
31 26 26 19
BspEI T'CCGG_A
Cuts at: 0 3639 4020
Size: 3639 381
BspHI T'CATG_A
Cuts at: 0 3923 4020
Size: 3923 97
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 288 3180 3296 4020
Size: 288 2892 116 724
Fragments arranged by size:
2892 724 288 116
BsrDI GCAATG_nn'
Cuts at: 0 2834 4020
Size: 2834 1186
BsrFI r'CCGG_y
Cuts at: 0 2213 4020
Size: 2213 1807
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3538 3994 4020
Size: 3538 456 26
Fragments arranged by size:
3538 456 26
BstYI r'GATC_y
Cuts at: 0 756 1957 2612 3396 4020
Size: 756 1201 655 784 624
Fragments arranged by size:
1201 784 756 655 624
Bsu36I CC'TnA_GG
Cuts at: 0 2009 4020
Size: 2009 2011
BtgI C'CryG_G
Cuts at: 0 249 1721 2642 2951 3032 3803 4020
Size: 249 1472 921 309 81 771 217
Fragments arranged by size:
1472 921 771 309 249 217 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2429 4020
Size: 2429 1591
BtsI GCAGTG_nn'
Cuts at: 0 667 722 1264 2074 4020
Size: 667 55 542 810 1946
Fragments arranged by size:
1946 810 667 542 55
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2933 2968 3014 3049 4020
Size: 2933 35 46 35 971
Fragments arranged by size:
2933 971 46 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 931 4020
Size: 931 3089
DrdI GACnn_nn'nnGTC
Cuts at: 0 1279 4020
Size: 1279 2741
EaeI y'GGCC_r
Cuts at: 0 252 329 500 1552 1599 1623 1718 2873
Size: 252 77 171 1052 47 24 95 1155
Cuts at: 2873 3273 3768 4020
Size: 400 495 252
Fragments arranged by size:
1155 1052 495 400 252 252 171 95
77 47 24
EarI CTCTTCn'nnn_
Cuts at: 0 753 1214 1219 1658 2586 4020
Size: 753 461 5 439 928 1434
Fragments arranged by size:
1434 928 753 461 439 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 761 2319 4020
Size: 761 1558 1701
Fragments arranged by size:
1701 1558 761
EcoICRI GAG'CTC
Cuts at: 0 29 1060 4020
Size: 29 1031 2960
Fragments arranged by size:
2960 1031 29
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 278 734 865 1085 1252 1562 1682 2267
Size: 278 456 131 220 167 310 120 585
Cuts at: 2267 2386 2584 2750 2882 3152 3347 3440 3857
Size: 119 198 166 132 270 195 93 417
Cuts at: 3857 4020
Size: 163
Fragments arranged by size:
585 456 417 310 278 270 220 198
195 167 166 163 132 131 120 119
93
EcoNI CCTnn'n_nnAGG
Cuts at: 0 641 832 1027 1346 1625 4020
Size: 641 191 195 319 279 2395
Fragments arranged by size:
2395 641 319 279 195 191
EcoO109I rG'GnC_Cy
Cuts at: 0 1033 1040 1150 1315 1827 1828 1882 2005
Size: 1033 7 110 165 512 1 54 123
Cuts at: 2005 2116 3037 3060 3070 3110 3265 3331 3340
Size: 111 921 23 10 40 155 66 9
Cuts at: 3340 3535 3724 3832 3907 3944 3991 4020
Size: 195 189 108 75 37 47 29
Fragments arranged by size:
1033 921 512 195 189 165 155 123
111 110 108 75 66 54 47 40
37 29 23 10 9 7 1
EcoRI G'AATT_C
Cuts at: 0 516 913 1865 4020
Size: 516 397 952 2155
Fragments arranged by size:
2155 952 516 397
EcoRV GAT'ATC
Cuts at: 0 449 882 4020
Size: 449 433 3138
Fragments arranged by size:
3138 449 433
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3286 4020
Size: 3286 734
HaeII r_GCGC'y
Cuts at: 0 433 861 1779 4020
Size: 433 428 918 2241
Fragments arranged by size:
2241 918 433 428
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 69 101 814 846 1269 1301 1962 1994
Size: 69 32 713 32 423 32 661 32
Cuts at: 1994 2568 2600 2875 2907 3006 3038 3117 3149
Size: 574 32 275 32 99 32 79 32
Cuts at: 3149 3711 3743 3774 3806 4020
Size: 562 32 31 32 214
Fragments arranged by size:
713 661 574 562 423 275 214 99
79 69 32 32 32 32 32 32
32 32 32 32 31
HincII GTy'rAC
Cuts at: 0 1950 4020
Size: 1950 2070
KpnI G_GTAC'C
Cuts at: 0 3133 4020
Size: 3133 887
MfeI C'AATT_G
Cuts at: 0 2186 4020
Size: 2186 1834
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1985 3438 3732 4020
Size: 1985 1453 294 288
Fragments arranged by size:
1985 1453 294 288
MscI TGG'CCA
Cuts at: 0 254 331 1554 1601 1720 2875 4020
Size: 254 77 1223 47 119 1155 1145
Fragments arranged by size:
1223 1155 1145 254 119 77 47
MslI CAynn'nnrTG
Cuts at: 0 1085 1747 2571 3781 3883 4020
Size: 1085 662 824 1210 102 137
Fragments arranged by size:
1210 1085 824 662 137 102
MspA1I CmG'CkG
Cuts at: 0 123 843 1616 1683 1698 2451 2476 3796
Size: 123 720 773 67 15 753 25 1320
Cuts at: 3796 4020
Size: 224
Fragments arranged by size:
1320 773 753 720 224 123 67 25
15
NaeI GCC'GGC
Cuts at: 0 2215 4020
Size: 2215 1805
NcoI C'CATG_G
Cuts at: 0 249 1721 2642 4020
Size: 249 1472 921 1378
Fragments arranged by size:
1472 1378 921 249
NdeI CA'TA_TG
Cuts at: 0 1169 2225 4020
Size: 1169 1056 1795
Fragments arranged by size:
1795 1169 1056
NgoMIV G'CCGG_C
Cuts at: 0 2213 4020
Size: 2213 1807
NsiI A_TGCA'T
Cuts at: 0 5 4020
Size: 5 4015
NspI r_CATG'y
Cuts at: 0 894 1398 1443 2049 2463 3375 3561 4020
Size: 894 504 45 606 414 912 186 459
Fragments arranged by size:
912 894 606 504 459 414 186 45
PasI CC'CwG_GG
Cuts at: 0 732 1711 3156 3531 3856 4020
Size: 732 979 1445 375 325 164
Fragments arranged by size:
1445 979 732 375 325 164
PfoI T'CCnGG_A
Cuts at: 0 1268 3537 4020
Size: 1268 2269 483
Fragments arranged by size:
2269 1268 483
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2779 2811 3494 3526 4020
Size: 2779 32 683 32 494
Fragments arranged by size:
2779 683 494 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1033 1150 1315 3037 3060 3110 3340 3535
Size: 1033 117 165 1722 23 50 230 195
Cuts at: 3535 3724 3907 3991 4020
Size: 189 183 84 29
Fragments arranged by size:
1722 1033 230 195 189 183 165 117
84 50 29 23
PshAI GACnn'nnGTC
Cuts at: 0 724 4020
Size: 724 3296
PspOMI G'GGCC_C
Cuts at: 0 688 1039 1827 1881 2004 2115 2191 2469
Size: 688 351 788 54 123 111 76 278
Cuts at: 2469 4020
Size: 1551
Fragments arranged by size:
1551 788 688 351 278 123 111 76
54
PspXI vC'TCGA_Gb
Cuts at: 0 3500 4020
Size: 3500 520
PstI C_TGCA'G
Cuts at: 0 345 542 1205 3404 4020
Size: 345 197 663 2199 616
Fragments arranged by size:
2199 663 616 345 197
PvuII CAG'CTG
Cuts at: 0 123 843 1683 1698 2451 2476 3796 4020
Size: 123 720 840 15 753 25 1320 224
Fragments arranged by size:
1320 840 753 720 224 123 25 15
SacI G_AGCT'C
Cuts at: 0 31 1062 4020
Size: 31 1031 2958
Fragments arranged by size:
2958 1031 31
SanDI GG'GwC_CC
Cuts at: 0 3037 3060 3110 3907 4020
Size: 3037 23 50 797 113
Fragments arranged by size:
3037 797 113 50 23
SapI GCTCTTCn'nnn_
Cuts at: 0 753 4020
Size: 753 3267
SexAI A'CCwGG_T
Cuts at: 0 1530 4020
Size: 1530 2490
SfcI C'TryA_G
Cuts at: 0 92 221 341 538 641 1201 2696 3400
Size: 92 129 120 197 103 560 1495 704
Cuts at: 3400 4020
Size: 620
Fragments arranged by size:
1495 704 620 560 197 129 120 103
92
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1006 3272 4020
Size: 1006 2266 748
Fragments arranged by size:
2266 1006 748
SmaI CCC'GGG
Cuts at: 0 3336 3949 4020
Size: 3336 613 71
Fragments arranged by size:
3336 613 71
SmlI C'TyrA_G
Cuts at: 0 1073 1897 2425 2539 3134 3500 4020
Size: 1073 824 528 114 595 366 520
Fragments arranged by size:
1073 824 595 528 520 366 114
SphI G_CATG'C
Cuts at: 0 894 2463 3375 4020
Size: 894 1569 912 645
Fragments arranged by size:
1569 912 894 645
SspI AAT'ATT
Cuts at: 0 3493 4020
Size: 3493 527
StyI C'CwwG_G
Cuts at: 0 249 1023 1650 1721 2312 2642 3270 3987
Size: 249 774 627 71 591 330 628 717
Cuts at: 3987 4020
Size: 33
Fragments arranged by size:
774 717 628 627 591 330 249 71
33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 567 585 3865 3964 4020
Size: 567 18 3280 99 56
Fragments arranged by size:
3280 567 99 56 18
TatI w'GTAC_w
Cuts at: 0 2746 3171 4020
Size: 2746 425 849
Fragments arranged by size:
2746 849 425
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 157 189 2265 2297 4020
Size: 157 32 2076 32 1723
Fragments arranged by size:
2076 1723 157 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1532 4020
Size: 1532 2488
XbaI T'CTAG_A
Cuts at: 0 1525 4020
Size: 1525 2495
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1786 2104 4020
Size: 1786 318 1916
Fragments arranged by size:
1916 1786 318
XhoI C'TCGA_G
Cuts at: 0 3500 4020
Size: 3500 520
XmaI C'CCGG_G
Cuts at: 0 3334 3947 4020
Size: 3334 613 73
Fragments arranged by size:
3334 613 73
XmnI GAAnn'nnTTC
Cuts at: 0 913 1112 4020
Size: 913 199 2908
Fragments arranged by size:
2908 913 199
Enzymes that do cut:
AarI Acc65I AcuI AfeI AflIII AlfI AloI AlwNI
ApaI ApoI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaWI BsaXI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrDI BsrFI BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraIII DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
HaeII Hin4I HincII KpnI MfeI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NsiI NspI PasI
PfoI PpiI PpuMI PshAI PspOMI PspXI PstI PvuII
SacI SanDI SapI SexAI SfcI SfiI SmaI SmlI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI AclI AflII AgeI AhdI AleI ApaLI
AscI AseI AsiSI BaeI BbeI BciVI BclI BmtI
BsaHI BsgI BsiEI BsiWI BsrBI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstZ17I ClaI DraI EagI FseI
FspI FspAI HindIII HpaI KasI MluI NarI NheI
NotI NruI PacI PciI PflMI PmeI PmlI PsiI
PsrI PvuI RsrII SacII SalI SbfI ScaI SfoI
SgfI SgrAI SnaBI SpeI SrfI StuI SwaI ZraI