Restriction map for
pF1KB7003
(Linear) (Six-base) MAPSORT of: KIBB7003.seq Check: 1309 from: 1 to: 3861
KIBB7003.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1533 1799 2398 3861
Size: 1533 266 599 1463
Fragments arranged by size:
1533 1463 599 266
AccI GT'mk_AC
Cuts at: 0 2878 3861
Size: 2878 983
Acc65I G'GTAC_C
Cuts at: 0 63 3861
Size: 63 3798
AclI AA'CG_TT
Cuts at: 0 1802 2924 3861
Size: 1802 1122 937
Fragments arranged by size:
1802 1122 937
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 420 1744 1922 2191 3861
Size: 420 1324 178 269 1670
Fragments arranged by size:
1670 1324 420 269 178
AfeI AGC'GCT
Cuts at: 0 1068 3861
Size: 1068 2793
AflII C'TTAA_G
Cuts at: 0 2995 3156 3861
Size: 2995 161 705
Fragments arranged by size:
2995 705 161
AflIII A'CryG_T
Cuts at: 0 3341 3861
Size: 3341 520
AleI CACnn'nnGTG
Cuts at: 0 607 1934 3040 3074 3861
Size: 607 1327 1106 34 787
Fragments arranged by size:
1327 1106 787 607 34
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3457 3489 3861
Size: 3457 32 372
Fragments arranged by size:
3457 372 32
AlwNI CAG_nnn'CTG
Cuts at: 0 886 1273 1624 2244 3861
Size: 886 387 351 620 1617
Fragments arranged by size:
1617 886 620 387 351
ApaLI G'TGCA_C
Cuts at: 0 92 3861
Size: 92 3769
ApoI r'AATT_y
Cuts at: 0 222 553 782 891 1116 1851 2456 2475
Size: 222 331 229 109 225 735 605 19
Cuts at: 2475 2587 2807 3804 3861
Size: 112 220 997 57
Fragments arranged by size:
997 735 605 331 229 225 222 220
112 109 57 19
AseI AT'TA_AT
Cuts at: 0 2492 3293 3533 3861
Size: 2492 801 240 328
Fragments arranged by size:
2492 801 328 240
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3563 3596 3861
Size: 3563 33 265
Fragments arranged by size:
3563 265 33
BamHI G'GATC_C
Cuts at: 0 1879 3861
Size: 1879 1982
BanI G'GyrC_C
Cuts at: 0 63 2719 3814 3861
Size: 63 2656 1095 47
Fragments arranged by size:
2656 1095 63 47
BbsI GAAGACnn'nnnn_
Cuts at: 0 184 1127 1679 1752 2285 2304 2324 2503
Size: 184 943 552 73 533 19 20 179
Cuts at: 2503 3861
Size: 1358
Fragments arranged by size:
1358 943 552 533 184 179 73 20
19
BciVI GTATCCnnnnn_n'
Cuts at: 0 1661 2619 3861
Size: 1661 958 1242
Fragments arranged by size:
1661 1242 958
BclI T'GATC_A
Cuts at: 0 1173 3861
Size: 1173 2688
BfrBI ATG'CAT
Cuts at: 0 1963 2164 3089 3861
Size: 1963 201 925 772
Fragments arranged by size:
1963 925 772 201
BlpI GC'TnA_GC
Cuts at: 0 206 3861
Size: 206 3655
Bme1580I G_kGCm'C
Cuts at: 0 96 3861
Size: 96 3765
BmrI ACTGGGnnnn_n'
Cuts at: 0 2546 3861
Size: 2546 1315
BmtI G_CTAG'C
Cuts at: 0 2390 3861
Size: 2390 1471
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2445 3861
Size: 2445 1416
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 1460 2141 3861
Size: 137 1323 681 1720
Fragments arranged by size:
1720 1323 681 137
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 659 672 1473 2013 3861
Size: 374 285 13 801 540 1848
Fragments arranged by size:
1848 801 540 374 285 13
BsaI GGTCTCn'nnnn_
Cuts at: 0 1989 3861
Size: 1989 1872
BsaBI GATnn'nnATC
Cuts at: 0 2165 3861
Size: 2165 1696
BseYI C'CCAG_C
Cuts at: 0 1400 3861
Size: 1400 2461
BsiEI CG_ry'CG
Cuts at: 0 3253 3861
Size: 3253 608
BsiHKAI G_wGCw'C
Cuts at: 0 96 2380 3861
Size: 96 2284 1481
Fragments arranged by size:
2284 1481 96
BsmI GAATG_Cn'
Cuts at: 0 328 949 1917 1963 2406 2911 3861
Size: 328 621 968 46 443 505 950
Fragments arranged by size:
968 950 621 505 443 328 46
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3841 3861
Size: 3841 20
Bsp1286I G_dGCh'C
Cuts at: 0 96 2380 3861
Size: 96 2284 1481
Fragments arranged by size:
2284 1481 96
BspHI T'CATG_A
Cuts at: 0 1593 2901 3861
Size: 1593 1308 960
Fragments arranged by size:
1593 1308 960
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1533 1799 2398 3861
Size: 1533 266 599 1463
Fragments arranged by size:
1533 1463 599 266
BsrBI CCG'CTC
Cuts at: 0 3152 3861
Size: 3152 709
BsrDI GCAATG_nn'
Cuts at: 0 1390 2366 2570 3861
Size: 1390 976 204 1291
Fragments arranged by size:
1390 1291 976 204
BsrGI T'GTAC_A
Cuts at: 0 1635 3861
Size: 1635 2226
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2105 2769 2778 3861
Size: 2105 664 9 1083
Fragments arranged by size:
2105 1083 664 9
BstBI TT'CG_AA
Cuts at: 0 3690 3861
Size: 3690 171
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3081 3171 3861
Size: 3081 90 690
Fragments arranged by size:
3081 690 90
BstYI r'GATC_y
Cuts at: 0 1256 1879 3861
Size: 1256 623 1982
Fragments arranged by size:
1982 1256 623
Bsu36I CC'TnA_GG
Cuts at: 0 818 2000 3469 3861
Size: 818 1182 1469 392
Fragments arranged by size:
1469 1182 818 392
BtgI C'CryG_G
Cuts at: 0 710 3861
Size: 710 3151
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2030 3861
Size: 2030 1831
BtsI GCAGTG_nn'
Cuts at: 0 1503 1777 3110 3399 3686 3861
Size: 1503 274 1333 289 287 175
Fragments arranged by size:
1503 1333 289 287 274 175
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 146 181 2945 2980 3861
Size: 146 35 2764 35 881
Fragments arranged by size:
2764 881 146 35 35
DraI TTT'AAA
Cuts at: 0 449 597 3215 3861
Size: 449 148 2618 646
Fragments arranged by size:
2618 646 449 148
DraIII CAC_nnn'GTG
Cuts at: 0 2105 3861
Size: 2105 1756
EaeI y'GGCC_r
Cuts at: 0 2667 3861
Size: 2667 1194
EarI CTCTTCn'nnn_
Cuts at: 0 356 1640 2555 2622 3364 3407 3861
Size: 356 1284 915 67 742 43 454
Fragments arranged by size:
1284 915 742 454 356 67 43
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 420 1460 1744 1922 2141 2191 3861
Size: 137 283 1040 284 178 219 50 1670
Fragments arranged by size:
1670 1040 284 283 219 178 137 50
EcoRI G'AATT_C
Cuts at: 0 3804 3861
Size: 3804 57
EcoRV GAT'ATC
Cuts at: 0 703 3861
Size: 703 3158
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2081 3861
Size: 2081 1780
HaeII r_GCGC'y
Cuts at: 0 1070 3861
Size: 1070 2791
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 110 142 276 308 506 538 645 677
Size: 110 32 134 32 198 32 107 32
Cuts at: 677 1165 1197 1755 1787 1882 1914 2860 2892
Size: 488 32 558 32 95 32 946 32
Cuts at: 2892 3365 3397 3861
Size: 473 32 464
Fragments arranged by size:
946 558 488 473 464 198 134 110
107 95 32 32 32 32 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 3632 3861
Size: 3632 229
HindIII A'AGCT_T
Cuts at: 0 160 3861
Size: 160 3701
KpnI G_GTAC'C
Cuts at: 0 67 3861
Size: 67 3794
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 210 655 722 1271 1284 1857 2210 2636
Size: 210 445 67 549 13 573 353 426
Cuts at: 2636 3861
Size: 1225
Fragments arranged by size:
1225 573 549 445 426 353 210 67
13
MscI TGG'CCA
Cuts at: 0 2669 3861
Size: 2669 1192
MslI CAynn'nnrTG
Cuts at: 0 87 607 1934 2371 2575 3040 3074 3861
Size: 87 520 1327 437 204 465 34 787
Fragments arranged by size:
1327 787 520 465 437 204 87 34
MspA1I CmG'CkG
Cuts at: 0 2673 3228 3861
Size: 2673 555 633
Fragments arranged by size:
2673 633 555
NcoI C'CATG_G
Cuts at: 0 710 3861
Size: 710 3151
NdeI CA'TA_TG
Cuts at: 0 2072 3861
Size: 2072 1789
NheI G'CTAG_C
Cuts at: 0 2386 3861
Size: 2386 1475
NsiI A_TGCA'T
Cuts at: 0 1965 2166 3091 3861
Size: 1965 201 925 770
Fragments arranged by size:
1965 925 770 201
NspI r_CATG'y
Cuts at: 0 2112 2694 2767 3089 3129 3345 3861
Size: 2112 582 73 322 40 216 516
Fragments arranged by size:
2112 582 516 322 216 73 40
PciI A'CATG_T
Cuts at: 0 3341 3861
Size: 3341 520
PsiI TTA'TAA
Cuts at: 0 692 3861
Size: 692 3169
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1748 1780 2630 2662 3861
Size: 1748 32 850 32 1199
Fragments arranged by size:
1748 1199 850 32 32
PstI C_TGCA'G
Cuts at: 0 3681 3861
Size: 3681 180
PvuI CG_AT'CG
Cuts at: 0 3253 3861
Size: 3253 608
PvuII CAG'CTG
Cuts at: 0 2673 3228 3861
Size: 2673 555 633
Fragments arranged by size:
2673 633 555
SexAI A'CCwGG_T
Cuts at: 0 40 3861
Size: 40 3821
SfcI C'TryA_G
Cuts at: 0 147 457 810 1985 3197 3677 3861
Size: 147 310 353 1175 1212 480 184
Fragments arranged by size:
1212 1175 480 353 310 184 147
SmlI C'TyrA_G
Cuts at: 0 389 651 674 1452 1992 2995 3156 3861
Size: 389 262 23 778 540 1003 161 705
Fragments arranged by size:
1003 778 705 540 389 262 161 23
SphI G_CATG'C
Cuts at: 0 2112 3089 3861
Size: 2112 977 772
Fragments arranged by size:
2112 977 772
SspI AAT'ATT
Cuts at: 0 669 2037 3211 3451 3591 3861
Size: 669 1368 1174 240 140 270
Fragments arranged by size:
1368 1174 669 270 240 140
StyI C'CwwG_G
Cuts at: 0 485 710 755 2858 3012 3861
Size: 485 225 45 2103 154 849
Fragments arranged by size:
2103 849 485 225 154 45
SwaI ATTT'AAAT
Cuts at: 0 3215 3861
Size: 3215 646
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1946 2133 3861
Size: 1946 187 1728
Fragments arranged by size:
1946 1728 187
TatI w'GTAC_w
Cuts at: 0 1635 1952 3861
Size: 1635 317 1909
Fragments arranged by size:
1909 1635 317
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2496 2528 3702 3734 3861
Size: 2496 32 1174 32 127
Fragments arranged by size:
2496 1174 127 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2127 3861
Size: 2127 1734
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 537 3861
Size: 537 3324
XmnI GAAnn'nnTTC
Cuts at: 0 1907 3861
Size: 1907 1954
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AleI AloI AlwNI ApaLI ApoI AseI BaeI BamHI
BanI BbsI BciVI BclI BfrBI BlpI Bme1580I BmrI
BmtI BplI BpmI BpuEI BsaI BsaBI BseYI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrGI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII EaeI EarI Eco57MI EcoRI
EcoRV FalI HaeII Hin4I HincII HindIII KpnI MmeI
MscI MslI MspA1I NcoI NdeI NheI NsiI NspI
PciI PsiI PsrI PstI PvuI PvuII SexAI SfcI
SmlI SphI SspI StyI SwaI TaqII TatI TstI
Tth111I XcmI XmnI
Enzymes that do not cut:
AatII AgeI AhdI AlfI ApaI AscI AsiSI AvaI
AvrII BanII BbeI BbvCI BcgI BglI BglII BmgBI
Bpu10I BsaAI BsaHI BsaWI BsaXI BseRI BsgI BsiWI
BspEI BsrFI BssHII BssSI BstEII BstZ17I ClaI DrdI
EagI EciI EcoICRI EcoNI EcoO109I FseI FspI FspAI
HpaI KasI MfeI MluI NaeI NarI NgoMIV NotI
NruI PacI PasI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PspOMI PspXI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI StuI XbaI XhoI XmaI
ZraI