Restriction map for
pF1KB7002
(Linear) (Six-base) MAPSORT of: KIBB7002.seq Check: 2178 from: 1 to: 9081
KIBB7002.orf
With 182 enzymes: SgfI *
December 28, 2009 14:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 8628 9081
Size: 8628 453
AatII G_ACGT'C
Cuts at: 0 5478 9081
Size: 5478 3603
AccI GT'mk_AC
Cuts at: 0 188 4708 8030 9081
Size: 188 4520 3322 1051
Fragments arranged by size:
4520 3322 1051 188
Acc65I G'GTAC_C
Cuts at: 0 1383 9081
Size: 1383 7698
AclI AA'CG_TT
Cuts at: 0 7173 9081
Size: 7173 1908
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 452 3085 3890 5789 6193 6260 6320 8837
Size: 452 2633 805 1899 404 67 60 2517
Cuts at: 8837 9081
Size: 244
Fragments arranged by size:
2633 2517 1899 805 452 404 244 67
60
AfeI AGC'GCT
Cuts at: 0 79 9081
Size: 79 9002
AflIII A'CryG_T
Cuts at: 0 2648 3368 4763 7092 9081
Size: 2648 720 1395 2329 1989
Fragments arranged by size:
2648 2329 1989 1395 720
AleI CACnn'nnGTG
Cuts at: 0 6973 9081
Size: 6973 2108
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 751 1070 8664 9081
Size: 751 319 7594 417
Fragments arranged by size:
7594 751 417 319
AlwNI CAG_nnn'CTG
Cuts at: 0 18 1833 5781 6238 7292 7693 8470 8839
Size: 18 1815 3948 457 1054 401 777 369
Cuts at: 8839 8968 9081
Size: 129 113
Fragments arranged by size:
3948 1815 1054 777 457 401 369 129
113 18
ApaLI G'TGCA_C
Cuts at: 0 3152 7088 9081
Size: 3152 3936 1993
Fragments arranged by size:
3936 3152 1993
ApoI r'AATT_y
Cuts at: 0 279 441 523 541 1586 2369 2655 3586
Size: 279 162 82 18 1045 783 286 931
Cuts at: 3586 4624 5200 6085 6277 6342 6912 7446 7808
Size: 1038 576 885 192 65 570 534 362
Cuts at: 7808 8012 9070 9081
Size: 204 1058 11
Fragments arranged by size:
1058 1045 1038 931 885 783 576 570
534 362 286 279 204 192 162 82
65 18 11
AseI AT'TA_AT
Cuts at: 0 869 2183 5368 5549 8231 9081
Size: 869 1314 3185 181 2682 850
Fragments arranged by size:
3185 2682 1314 869 850 181
AvaI C'yCGr_G
Cuts at: 0 692 9081
Size: 692 8389
AvrII C'CTAG_G
Cuts at: 0 739 9081
Size: 739 8342
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2770 2803 3955 3988 8597 8630 9081
Size: 2770 33 1152 33 4609 33 451
Fragments arranged by size:
4609 2770 1152 451 33 33 33
BamHI G'GATC_C
Cuts at: 0 354 1014 2088 6421 9081
Size: 354 660 1074 4333 2660
Fragments arranged by size:
4333 2660 1074 660 354
BanI G'GyrC_C
Cuts at: 0 1383 7230 9081
Size: 1383 5847 1851
Fragments arranged by size:
5847 1851 1383
BanII G_rGCy'C
Cuts at: 0 4686 7286 8289 9081
Size: 4686 2600 1003 792
Fragments arranged by size:
4686 2600 1003 792
BbsI GAAGACnn'nnnn_
Cuts at: 0 1162 2510 3449 5747 7513 8526 8875 9081
Size: 1162 1348 939 2298 1766 1013 349 206
Fragments arranged by size:
2298 1766 1348 1162 1013 939 349 206
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1837 1871 7953 7987 9081
Size: 1837 34 6082 34 1094
Fragments arranged by size:
6082 1837 1094 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 5688 8598 9081
Size: 5688 2910 483
Fragments arranged by size:
5688 2910 483
BclI T'GATC_A
Cuts at: 0 1425 5397 9081
Size: 1425 3972 3684
Fragments arranged by size:
3972 3684 1425
BfrBI ATG'CAT
Cuts at: 0 94 482 1978 2435 3228 3705 4862 5298
Size: 94 388 1496 457 793 477 1157 436
Cuts at: 5298 6693 8641 9081
Size: 1395 1948 440
Fragments arranged by size:
1948 1496 1395 1157 793 477 457 440
436 388 94
BglII A'GATC_T
Cuts at: 0 456 3205 9081
Size: 456 2749 5876
Fragments arranged by size:
5876 2749 456
BlpI GC'TnA_GC
Cuts at: 0 7310 9081
Size: 7310 1771
Bme1580I G_kGCm'C
Cuts at: 0 3156 3578 7092 9081
Size: 3156 422 3514 1989
Fragments arranged by size:
3514 3156 1989 422
BmgBI CAC'GTC
Cuts at: 0 2076 3032 9081
Size: 2076 956 6049
Fragments arranged by size:
6049 2076 956
BmrI ACTGGGnnnn_n'
Cuts at: 0 127 2087 2128 2234 6927 9081
Size: 127 1960 41 106 4693 2154
Fragments arranged by size:
4693 2154 1960 127 106 41
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2824 4705 5832 6549 9081
Size: 2824 1881 1127 717 2532
Fragments arranged by size:
2824 2532 1881 1127 717
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 608 2177 2738 2794 3018 3795 5874 7411
Size: 608 1569 561 56 224 777 2079 1537
Cuts at: 7411 7907 8963 9081
Size: 496 1056 118
Fragments arranged by size:
2079 1569 1537 1056 777 608 561 496
224 118 56
Bpu10I CC'TnA_GC
Cuts at: 0 4448 4457 4533 7280 8483 9081
Size: 4448 9 76 2747 1203 598
Fragments arranged by size:
4448 2747 1203 598 76 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1377 2545 2609 3298 5164 8273 9081
Size: 1377 1168 64 689 1866 3109 808
Fragments arranged by size:
3109 1866 1377 1168 808 689 64
BsaI GGTCTCn'nnnn_
Cuts at: 0 4121 4145 9081
Size: 4121 24 4936
Fragments arranged by size:
4936 4121 24
BsaAI yAC'GTr
Cuts at: 0 5236 9081
Size: 5236 3845
BsaBI GATnn'nnATC
Cuts at: 0 1019 1286 7421 9081
Size: 1019 267 6135 1660
Fragments arranged by size:
6135 1660 1019 267
BsaHI Gr'CG_yC
Cuts at: 0 5475 9081
Size: 5475 3606
BsaWI w'CCGG_w
Cuts at: 0 1159 4835 9081
Size: 1159 3676 4246
Fragments arranged by size:
4246 3676 1159
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 37 67 4504 4534 7719 7749 8195 8225
Size: 37 30 4437 30 3185 30 446 30
Cuts at: 8225 8654 8684 8968 8998 9081
Size: 429 30 284 30 83
Fragments arranged by size:
4437 3185 446 429 284 83 37 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 978 2174 6535 7372 8216 8538 9081
Size: 978 1196 4361 837 844 322 543
Fragments arranged by size:
4361 1196 978 844 837 543 322
BseYI C'CCAG_C
Cuts at: 0 556 1092 4060 9081
Size: 556 536 2968 5021
Fragments arranged by size:
5021 2968 556 536
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2117 9081
Size: 2117 6964
BsiHKAI G_wGCw'C
Cuts at: 0 2952 3156 3775 4686 5358 5592 7092 7286
Size: 2952 204 619 911 672 234 1500 194
Cuts at: 7286 7496 8289 9081
Size: 210 793 792
Fragments arranged by size:
2952 1500 911 793 792 672 619 234
210 204 194
BsmI GAATG_Cn'
Cuts at: 0 2044 2623 3895 4162 5055 9081
Size: 2044 579 1272 267 893 4026
Fragments arranged by size:
4026 2044 1272 893 579 267
BsmBI CGTCTCn'nnnn_
Cuts at: 0 5466 9081
Size: 5466 3615
Bsp1286I G_dGCh'C
Cuts at: 0 2952 3156 3578 3775 4686 5358 5592 7092
Size: 2952 204 422 197 911 672 234 1500
Cuts at: 7092 7286 7496 8289 9081
Size: 194 210 793 792
Fragments arranged by size:
2952 1500 911 793 792 672 422 234
210 204 197 194
BspEI T'CCGG_A
Cuts at: 0 4835 9081
Size: 4835 4246
BspHI T'CATG_A
Cuts at: 0 4098 5301 7530 7983 9081
Size: 4098 1203 2229 453 1098
Fragments arranged by size:
4098 2229 1203 1098 453
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 14 2813 7516 7678 8628 8656 9081
Size: 14 2799 4703 162 950 28 425
Fragments arranged by size:
4703 2799 950 425 162 28 14
BsrDI GCAATG_nn'
Cuts at: 0 489 4055 4940 9081
Size: 489 3566 885 4141
Fragments arranged by size:
4141 3566 885 489
BsrGI T'GTAC_A
Cuts at: 0 1173 6556 9081
Size: 1173 5383 2525
Fragments arranged by size:
5383 2525 1173
BssSI C'ACGA_G
Cuts at: 0 8099 9081
Size: 8099 982
BstAPI GCAn_nnn'nTGC
Cuts at: 0 15 2947 7491 7693 9081
Size: 15 2932 4544 202 1388
Fragments arranged by size:
4544 2932 1388 202 15
BstXI CCAn_nnnn'nTGG
Cuts at: 0 6878 8659 9081
Size: 6878 1781 422
Fragments arranged by size:
6878 1781 422
BstYI r'GATC_y
Cuts at: 0 354 456 1014 2088 2448 2762 3205 4002
Size: 354 102 558 1074 360 314 443 797
Cuts at: 4002 5797 6421 8836 9081
Size: 1795 624 2415 245
Fragments arranged by size:
2415 1795 1074 797 624 558 443 360
354 314 245 102
Bsu36I CC'TnA_GG
Cuts at: 0 4111 9081
Size: 4111 4970
BtgI C'CryG_G
Cuts at: 0 56 1214 1493 6426 6996 7629 8173 9081
Size: 56 1158 279 4933 570 633 544 908
Fragments arranged by size:
4933 1158 908 633 570 544 279 56
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 7492 9081
Size: 7492 1589
BtsI GCAGTG_nn'
Cuts at: 0 45 551 1042 2952 5579 6980 8509 9081
Size: 45 506 491 1910 2627 1401 1529 572
Fragments arranged by size:
2627 1910 1529 1401 572 506 491 45
ClaI AT'CG_AT
Cuts at: 0 5255 9081
Size: 5255 3826
DraI TTT'AAA
Cuts at: 0 446 1763 5045 5597 6050 6101 9044 9081
Size: 446 1317 3282 552 453 51 2943 37
Fragments arranged by size:
3282 2943 1317 552 453 446 51 37
DraIII CAC_nnn'GTG
Cuts at: 0 15 9081
Size: 15 9066
EaeI y'GGCC_r
Cuts at: 0 312 3076 4470 6880 7004 8018 9081
Size: 312 2764 1394 2410 124 1014 1063
Fragments arranged by size:
2764 2410 1394 1063 1014 312 124
EarI CTCTTCn'nnn_
Cuts at: 0 1145 2195 6212 6994 7332 7592 7667 7768
Size: 1145 1050 4017 782 338 260 75 101
Cuts at: 7768 8270 8813 9081
Size: 502 543 268
Fragments arranged by size:
4017 1145 1050 782 543 502 338 268
260 101 75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 621 9081
Size: 621 8460
EcoICRI GAG'CTC
Cuts at: 0 4684 7284 8287 9081
Size: 4684 2600 1003 794
Fragments arranged by size:
4684 2600 1003 794
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 452 608 2177 2738 2794 3018 3085 3795
Size: 452 156 1569 561 56 224 67 710
Cuts at: 3795 3890 5789 5874 6193 6260 6320 7411 7907
Size: 95 1899 85 319 67 60 1091 496
Cuts at: 7907 8837 8963 9081
Size: 930 126 118
Fragments arranged by size:
1899 1569 1091 930 710 561 496 452
319 224 156 126 118 95 85 67
67 60 56
EcoNI CCTnn'n_nnAGG
Cuts at: 0 10 9081
Size: 10 9071
EcoO109I rG'GnC_Cy
Cuts at: 0 3273 7690 8965 9081
Size: 3273 4417 1275 116
Fragments arranged by size:
4417 3273 1275 116
EcoRI G'AATT_C
Cuts at: 0 1586 6912 8012 9081
Size: 1586 5326 1100 1069
Fragments arranged by size:
5326 1586 1100 1069
EcoRV GAT'ATC
Cuts at: 0 997 1799 6666 7882 7923 9081
Size: 997 802 4867 1216 41 1158
Fragments arranged by size:
4867 1216 1158 997 802 41
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3115 4050 8267 9081
Size: 3115 935 4217 814
Fragments arranged by size:
4217 3115 935 814
HaeII r_GCGC'y
Cuts at: 0 81 9081
Size: 81 9000
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1288 1320 1603 1635 2172 2204 2754 2786
Size: 1288 32 283 32 537 32 550 32
Cuts at: 2786 3007 3039 3418 3450 3951 3983 4350 4382
Size: 221 32 379 32 501 32 367 32
Cuts at: 4382 4504 4536 5812 5844 6214 6246 6350 6382
Size: 122 32 1276 32 370 32 104 32
Cuts at: 6382 7500 7532 7610 7642 7666 7698 8241 8271
Size: 1118 32 78 32 24 32 543 30
Cuts at: 8271 8273 8303 8850 8882 9081
Size: 2 30 547 32 199
Fragments arranged by size:
1288 1276 1118 550 547 543 537 501
379 370 367 283 221 199 122 104
78 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 30 30 24 2
HincII GTy'rAC
Cuts at: 0 258 767 1665 2073 2130 2410 2828 8749
Size: 258 509 898 408 57 280 418 5921
Cuts at: 8749 9081
Size: 332
Fragments arranged by size:
5921 898 509 418 408 332 280 258
57
HindIII A'AGCT_T
Cuts at: 0 6837 7712 9081
Size: 6837 875 1369
Fragments arranged by size:
6837 1369 875
HpaI GTT'AAC
Cuts at: 0 258 9081
Size: 258 8823
KpnI G_GTAC'C
Cuts at: 0 1387 9081
Size: 1387 7694
MfeI C'AATT_G
Cuts at: 0 4544 5129 7486 9081
Size: 4544 585 2357 1595
Fragments arranged by size:
4544 2357 1595 585
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 940 4456 5714 5969 7638 8284 8836 9081
Size: 940 3516 1258 255 1669 646 552 245
Fragments arranged by size:
3516 1669 1258 940 646 552 255 245
MscI TGG'CCA
Cuts at: 0 314 3078 4472 6882 7006 8020 9081
Size: 314 2764 1394 2410 124 1014 1061
Fragments arranged by size:
2764 2410 1394 1061 1014 314 124
MslI CAynn'nnrTG
Cuts at: 0 99 1052 1639 1720 1811 1919 1973 3296
Size: 99 953 587 81 91 108 54 1323
Cuts at: 3296 3679 4987 5293 5509 6973 8099 8657 9081
Size: 383 1308 306 216 1464 1126 558 424
Fragments arranged by size:
1464 1323 1308 1126 953 587 558 424
383 306 216 108 99 91 81 54
MspA1I CmG'CkG
Cuts at: 0 613 1417 8116 8566 9081
Size: 613 804 6699 450 515
Fragments arranged by size:
6699 804 613 515 450
NcoI C'CATG_G
Cuts at: 0 1214 1493 6426 6996 7629 8173 9081
Size: 1214 279 4933 570 633 544 908
Fragments arranged by size:
4933 1214 908 633 570 544 279
NdeI CA'TA_TG
Cuts at: 0 4370 5510 8679 8730 9081
Size: 4370 1140 3169 51 351
Fragments arranged by size:
4370 3169 1140 351 51
NsiI A_TGCA'T
Cuts at: 0 96 484 1980 2437 3230 3707 4864 5300
Size: 96 388 1496 457 793 477 1157 436
Cuts at: 5300 6695 8643 9081
Size: 1395 1948 438
Fragments arranged by size:
1948 1496 1395 1157 793 477 457 438
436 388 96
NspI r_CATG'y
Cuts at: 0 1051 1522 1810 2282 2652 2945 3232 3372
Size: 1051 471 288 472 370 293 287 140
Cuts at: 3372 3709 4456 4767 6098 7096 8098 8172 8481
Size: 337 747 311 1331 998 1002 74 309
Cuts at: 8481 8911 9081
Size: 430 170
Fragments arranged by size:
1331 1051 1002 998 747 472 471 430
370 337 311 309 293 288 287 170
140 74
PciI A'CATG_T
Cuts at: 0 2648 3368 4763 7092 9081
Size: 2648 720 1395 2329 1989
Fragments arranged by size:
2648 2329 1989 1395 720
PflMI CCAn_nnn'nTGG
Cuts at: 0 7002 8179 8470 9081
Size: 7002 1177 291 611
Fragments arranged by size:
7002 1177 611 291
PfoI T'CCnGG_A
Cuts at: 0 3665 7427 7884 9081
Size: 3665 3762 457 1197
Fragments arranged by size:
3762 3665 1197 457
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 5445 5477 9081
Size: 5445 32 3604
Fragments arranged by size:
5445 3604 32
PpuMI rG'GwC_Cy
Cuts at: 0 3273 7690 9081
Size: 3273 4417 1391
Fragments arranged by size:
4417 3273 1391
PshAI GACnn'nnGTC
Cuts at: 0 8744 9081
Size: 8744 337
PsiI TTA'TAA
Cuts at: 0 3182 3751 9081
Size: 3182 569 5330
Fragments arranged by size:
5330 3182 569
PspXI vC'TCGA_Gb
Cuts at: 0 692 9081
Size: 692 8389
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3798 3830 5694 5726 9081
Size: 3798 32 1864 32 3355
Fragments arranged by size:
3798 3355 1864 32 32
PstI C_TGCA'G
Cuts at: 0 850 5094 5217 7689 9081
Size: 850 4244 123 2472 1392
Fragments arranged by size:
4244 2472 1392 850 123
PvuII CAG'CTG
Cuts at: 0 613 1417 8116 8566 9081
Size: 613 804 6699 450 515
Fragments arranged by size:
6699 804 613 515 450
SacI G_AGCT'C
Cuts at: 0 4686 7286 8289 9081
Size: 4686 2600 1003 792
Fragments arranged by size:
4686 2600 1003 792
SapI GCTCTTCn'nnn_
Cuts at: 0 6212 9081
Size: 6212 2869
ScaI AGT'ACT
Cuts at: 0 1442 6803 8824 9081
Size: 1442 5361 2021 257
Fragments arranged by size:
5361 2021 1442 257
SexAI A'CCwGG_T
Cuts at: 0 11 3126 9081
Size: 11 3115 5955
Fragments arranged by size:
5955 3115 11
SfcI C'TryA_G
Cuts at: 0 846 3137 3464 3623 5090 5213 5423 6247
Size: 846 2291 327 159 1467 123 210 824
Cuts at: 6247 6753 7685 8561 9081
Size: 506 932 876 520
Fragments arranged by size:
2291 1467 932 876 846 824 520 506
327 210 159 123
SmlI C'TyrA_G
Cuts at: 0 692 1392 2524 2624 3277 5143 8288 9081
Size: 692 700 1132 100 653 1866 3145 793
Fragments arranged by size:
3145 1866 1132 793 700 692 653 100
SnaBI TAC'GTA
Cuts at: 0 5236 9081
Size: 5236 3845
SpeI A'CTAG_T
Cuts at: 0 5698 7143 9081
Size: 5698 1445 1938
Fragments arranged by size:
5698 1938 1445
SphI G_CATG'C
Cuts at: 0 1051 2282 2945 4456 8481 9081
Size: 1051 1231 663 1511 4025 600
Fragments arranged by size:
4025 1511 1231 1051 663 600
SspI AAT'ATT
Cuts at: 0 171 2031 8934 9081
Size: 171 1860 6903 147
Fragments arranged by size:
6903 1860 171 147
StuI AGG'CCT
Cuts at: 0 3135 4703 9081
Size: 3135 1568 4378
Fragments arranged by size:
4378 3135 1568
StyI C'CwwG_G
Cuts at: 0 651 739 1214 1493 2538 4025 4590 4698
Size: 651 88 475 279 1045 1487 565 108
Cuts at: 4698 6426 6996 7629 8173 9081
Size: 1728 570 633 544 908
Fragments arranged by size:
1728 1487 1045 908 651 633 570 565
544 475 279 108 88
SwaI ATTT'AAAT
Cuts at: 0 446 9081
Size: 446 8635
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2242 4807 9081
Size: 2242 2565 4274
Fragments arranged by size:
4274 2565 2242
TatI w'GTAC_w
Cuts at: 0 759 1173 1440 1563 3591 3948 4424 6556
Size: 759 414 267 123 2028 357 476 2132
Cuts at: 6556 6801 8185 8822 9081
Size: 245 1384 637 259
Fragments arranged by size:
2132 2028 1384 759 637 476 414 357
267 259 245 123
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 37 69 1089 1121 5011 5043 8195 8227
Size: 37 32 1020 32 3890 32 3152 32
Cuts at: 8227 8930 8962 8968 9000 9081
Size: 703 32 6 32 81
Fragments arranged by size:
3890 3152 1020 703 81 37 32 32
32 32 32 32 6
XbaI T'CTAG_A
Cuts at: 0 3436 9081
Size: 3436 5645
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 414 2247 7755 8495 9081
Size: 414 1833 5508 740 586
Fragments arranged by size:
5508 1833 740 586 414
XhoI C'TCGA_G
Cuts at: 0 692 9081
Size: 692 8389
XmnI GAAnn'nnTTC
Cuts at: 0 897 914 1442 5256 6344 7604 7808 8250
Size: 897 17 528 3814 1088 1260 204 442
Cuts at: 8250 9081
Size: 831
Fragments arranged by size:
3814 1260 1088 897 831 528 442 204
17
ZraI GAC'GTC
Cuts at: 0 5476 9081
Size: 5476 3605
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AleI AlfI AlwNI ApaLI ApoI AseI AvaI AvrII
BaeI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrDI BsrGI BssSI BstAPI BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MmeI MscI MslI MspA1I NcoI NdeI NsiI NspI
PciI PflMI PfoI PpiI PpuMI PshAI PsiI PspXI
PsrI PstI PvuII SacI SapI ScaI SexAI SfcI
SmlI SnaBI SpeI SphI SspI StuI StyI SwaI
TaqII TatI TstI XbaI XcmI XhoI XmnI ZraI
Enzymes that do not cut:
AflII AgeI AhdI AloI ApaI AscI AsiSI BbeI
BbvCI BglI BmtI BsiEI BsiWI BsrBI BsrFI BssHII
BstBI BstEII BstZ17I CspCI DrdI EagI FseI FspI
FspAI KasI MluI NaeI NarI NgoMIV NheI NotI
NruI PacI PasI PmeI PmlI PspOMI PvuI RsrII
SacII SalI SanDI SbfI SfiI SfoI SgfI SgrAI
SmaI SrfI Tth111I XmaI