(Linear) (Six-base) MAPSORT of: KIBB6998.seq  Check: 2176  from: 1  to: 651



KIBB6998.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:07  ..

AatII G_ACGT'C

Cuts at:      0      22     651 
   Size:         22     629
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     558     651 
   Size:        558      93
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     331     651 
   Size:        331     320
 
BanI G'GyrC_C

Cuts at:      0      73     651 
   Size:         73     578
 
BanII G_rGCy'C

Cuts at:      0     344     651 
   Size:        344     307
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     470     651 
   Size:        470     181
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     491     651 
   Size:        491     160
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     358     651 
   Size:        358     293
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     478     532     651 
   Size:        478      54     119

  Fragments arranged by size: 

                 478     119      54
 
BsaBI GATnn'nnATC

Cuts at:      0     407     651 
   Size:        407     244
 
BsaHI Gr'CG_yC

Cuts at:      0      19     651 
   Size:         19     632
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     637     651 
   Size:        637      14
 
Bsp1286I G_dGCh'C

Cuts at:      0     344     651 
   Size:        344     307
 
BsrBI CCG'CTC

Cuts at:      0     626     651 
   Size:        626      25
 
BssHII G'CGCG_C

Cuts at:      0     134     651 
   Size:        134     517
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     378     651 
   Size:        378     273
 
BstYI r'GATC_y

Cuts at:      0     488     651 
   Size:        488     163
 
Bsu36I CC'TnA_GG

Cuts at:      0     355     651 
   Size:        355     296
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     115     416     651 
   Size:        115     301     235

  Fragments arranged by size: 

                 301     235     115
 
BtsI GCAGTG_nn'

Cuts at:      0     541     625     651 
   Size:        541      84      26

  Fragments arranged by size: 

                 541      84      26
 
EaeI y'GGCC_r

Cuts at:      0     146     651 
   Size:        146     505
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     358     558     651 
   Size:        358     200      93

  Fragments arranged by size: 

                 358     200      93
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     418     651 
   Size:        418     233
 
EcoO109I rG'GnC_Cy

Cuts at:      0     423     651 
   Size:        423     228
 
FspI TGC'GCA

Cuts at:      0     610     651 
   Size:        610      41
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     508     540     651 
   Size:        508      32     111

  Fragments arranged by size: 

                 508     111      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     277     287     651 
   Size:        277      10     364

  Fragments arranged by size: 

                 364     277      10
 
MscI TGG'CCA

Cuts at:      0     148     651 
   Size:        148     503
 
MslI CAynn'nnrTG

Cuts at:      0     533     651 
   Size:        533     118
 
MspA1I CmG'CkG

Cuts at:      0     144     641     651 
   Size:        144     497      10

  Fragments arranged by size: 

                 497     144      10
 
PfoI T'CCnGG_A

Cuts at:      0      13     651 
   Size:         13     638
 
PpuMI rG'GwC_Cy

Cuts at:      0     423     651 
   Size:        423     228
 
PvuII CAG'CTG

Cuts at:      0     144     641     651 
   Size:        144     497      10

  Fragments arranged by size: 

                 497     144      10
 
SexAI A'CCwGG_T

Cuts at:      0     377     651 
   Size:        377     274
 
SfcI C'TryA_G

Cuts at:      0     504     651 
   Size:        504     147
 
SmlI C'TyrA_G

Cuts at:      0     493     547     651 
   Size:        493      54     104

  Fragments arranged by size: 

                 493     104      54
 
Tth111I GACn'n_nGTC

Cuts at:      0     592     651 
   Size:        592      59
 
XbaI T'CTAG_A

Cuts at:      0     294     651 
   Size:        294     357
 
ZraI GAC'GTC

Cuts at:      0      20     651 
   Size:         20     631
 

 Enzymes that do cut:

    AatII     AcuI     AlfI     BanI    BanII     BbsI     BmrI     BpmI
    BpuEI    BsaBI    BsaHI     BsgI Bsp1286I    BsrBI   BssHII    BstXI
    BstYI   Bsu36I    BtgZI     BtsI     EaeI  Eco57MI    EcoNI EcoO109I
     FspI    Hin4I     MmeI     MscI     MslI   MspA1I     PfoI    PpuMI
    PvuII    SexAI     SfcI     SmlI  Tth111I     XbaI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaWI    BsaXI
    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI
   BstEII  BstZ17I     BtgI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI
     TstI     XcmI     XhoI     XmaI     XmnI