Restriction map for
pF1KB6996
(Linear) (Six-base) MAPSORT of: KIBB6996.seq Check: 8851 from: 1 to: 762
KIBB6996.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 455 509 762
Size: 455 54 253
Fragments arranged by size:
455 253 54
AflIII A'CryG_T
Cuts at: 0 173 762
Size: 173 589
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 221 253 762
Size: 221 32 509
Fragments arranged by size:
509 221 32
AlwNI CAG_nnn'CTG
Cuts at: 0 108 487 762
Size: 108 379 275
Fragments arranged by size:
379 275 108
ApoI r'AATT_y
Cuts at: 0 390 668 762
Size: 390 278 94
Fragments arranged by size:
390 278 94
BbsI GAAGACnn'nnnn_
Cuts at: 0 327 762
Size: 327 435
BmrI ACTGGGnnnn_n'
Cuts at: 0 157 762
Size: 157 605
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 601 762
Size: 601 161
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 762
Size: 262 500
BsaWI w'CCGG_w
Cuts at: 0 696 762
Size: 696 66
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 557 587 762
Size: 557 30 175
Fragments arranged by size:
557 175 30
BseYI C'CCAG_C
Cuts at: 0 23 270 762
Size: 23 247 492
Fragments arranged by size:
492 247 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 762
Size: 574 188
BspEI T'CCGG_A
Cuts at: 0 696 762
Size: 696 66
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 153 762
Size: 153 609
BsrDI GCAATG_nn'
Cuts at: 0 518 762
Size: 518 244
BsrGI T'GTAC_A
Cuts at: 0 120 603 762
Size: 120 483 159
Fragments arranged by size:
483 159 120
BstYI r'GATC_y
Cuts at: 0 484 762
Size: 484 278
BtgI C'CryG_G
Cuts at: 0 254 762
Size: 254 508
DraIII CAC_nnn'GTG
Cuts at: 0 165 762
Size: 165 597
EaeI y'GGCC_r
Cuts at: 0 11 544 762
Size: 11 533 218
Fragments arranged by size:
533 218 11
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 455 509 601 762
Size: 455 54 92 161
Fragments arranged by size:
455 161 92 54
EcoRI G'AATT_C
Cuts at: 0 668 762
Size: 668 94
FspI TGC'GCA
Cuts at: 0 55 762
Size: 55 707
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 718 750 762
Size: 718 32 12
Fragments arranged by size:
718 32 12
MspA1I CmG'CkG
Cuts at: 0 274 609 635 762
Size: 274 335 26 127
Fragments arranged by size:
335 274 127 26
NcoI C'CATG_G
Cuts at: 0 254 762
Size: 254 508
NdeI CA'TA_TG
Cuts at: 0 71 762
Size: 71 691
NspI r_CATG'y
Cuts at: 0 177 681 762
Size: 177 504 81
Fragments arranged by size:
504 177 81
PciI A'CATG_T
Cuts at: 0 173 762
Size: 173 589
PstI C_TGCA'G
Cuts at: 0 150 279 686 762
Size: 150 129 407 76
Fragments arranged by size:
407 150 129 76
PvuII CAG'CTG
Cuts at: 0 274 609 635 762
Size: 274 335 26 127
Fragments arranged by size:
335 274 127 26
SfcI C'TryA_G
Cuts at: 0 146 275 682 762
Size: 146 129 407 80
Fragments arranged by size:
407 146 129 80
SmlI C'TyrA_G
Cuts at: 0 241 762
Size: 241 521
StyI C'CwwG_G
Cuts at: 0 254 762
Size: 254 508
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 176 762
Size: 176 586
TatI w'GTAC_w
Cuts at: 0 120 603 762
Size: 120 483 159
Fragments arranged by size:
483 159 120
Enzymes that do cut:
AcuI AflIII AloI AlwNI ApoI BbsI BmrI BpmI
BpuEI BsaWI BsaXI BseYI BsgI BspEI BspMI BsrDI
BsrGI BstYI BtgI DraIII EaeI Eco57MI EcoRI FspI
Hin4I MspA1I NcoI NdeI NspI PciI PstI PvuII
SfcI SmlI StyI TaqII TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmtI BplI Bpu10I BsaI BsaAI BsaBI BsaHI
BseRI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspHI
BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI
EagI EarI EciI EcoICRI EcoNI EcoO109I EcoRV FalI
FseI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI NaeI NarI NgoMIV
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI