Restriction map for
pF1KB6991
(Linear) (Six-base) MAPSORT of: KIBB6991.seq Check: 985 from: 1 to: 663
KIBB6991.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 648 663
Size: 648 15
AccI GT'mk_AC
Cuts at: 0 50 273 663
Size: 50 223 390
Fragments arranged by size:
390 223 50
AclI AA'CG_TT
Cuts at: 0 478 663
Size: 478 185
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 283 663
Size: 71 212 380
Fragments arranged by size:
380 212 71
AfeI AGC'GCT
Cuts at: 0 364 663
Size: 364 299
AlwNI CAG_nnn'CTG
Cuts at: 0 106 543 663
Size: 106 437 120
Fragments arranged by size:
437 120 106
ApaI G_GGCC'C
Cuts at: 0 184 663
Size: 184 479
AvaI C'yCGr_G
Cuts at: 0 138 663
Size: 138 525
BanI G'GyrC_C
Cuts at: 0 483 663
Size: 483 180
BanII G_rGCy'C
Cuts at: 0 184 663
Size: 184 479
BbvCI CC'TCA_GC
Cuts at: 0 99 663
Size: 99 564
BglI GCCn_nnn'nGGC
Cuts at: 0 653 663
Size: 653 10
Bme1580I G_kGCm'C
Cuts at: 0 184 488 658 663
Size: 184 304 170 5
Fragments arranged by size:
304 184 170 5
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 430 663
Size: 430 233
Bpu10I CC'TnA_GC
Cuts at: 0 99 663
Size: 99 564
BsaBI GATnn'nnATC
Cuts at: 0 250 663
Size: 250 413
BseYI C'CCAG_C
Cuts at: 0 74 146 663
Size: 74 72 517
Fragments arranged by size:
517 74 72
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 532 663
Size: 253 279 131
Fragments arranged by size:
279 253 131
BsmBI CGTCTCn'nnnn_
Cuts at: 0 15 663
Size: 15 648
Bsp1286I G_dGCh'C
Cuts at: 0 184 488 658 663
Size: 184 304 170 5
Fragments arranged by size:
304 184 170 5
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 648 663
Size: 648 15
BsrBI CCG'CTC
Cuts at: 0 194 663
Size: 194 469
BstAPI GCAn_nnn'nTGC
Cuts at: 0 640 663
Size: 640 23
BtsI GCAGTG_nn'
Cuts at: 0 351 515 663
Size: 351 164 148
Fragments arranged by size:
351 164 148
DraIII CAC_nnn'GTG
Cuts at: 0 400 663
Size: 400 263
EarI CTCTTCn'nnn_
Cuts at: 0 377 663
Size: 377 286
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 283 430 663
Size: 71 212 147 233
Fragments arranged by size:
233 212 147 71
EcoO109I rG'GnC_Cy
Cuts at: 0 118 142 181 663
Size: 118 24 39 482
Fragments arranged by size:
482 118 39 24
HaeII r_GCGC'y
Cuts at: 0 366 663
Size: 366 297
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 12 44 427 459 663
Size: 12 32 383 32 204
Fragments arranged by size:
383 204 32 32 12
HindIII A'AGCT_T
Cuts at: 0 83 663
Size: 83 580
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 663
Size: 499 164
MspA1I CmG'CkG
Cuts at: 0 91 103 261 408 663
Size: 91 12 158 147 255
Fragments arranged by size:
255 158 147 91 12
PasI CC'CwG_GG
Cuts at: 0 649 663
Size: 649 14
PflMI CCAn_nnn'nTGG
Cuts at: 0 643 663
Size: 643 20
PpuMI rG'GwC_Cy
Cuts at: 0 118 142 663
Size: 118 24 521
Fragments arranged by size:
521 118 24
PsiI TTA'TAA
Cuts at: 0 242 663
Size: 242 421
PspOMI G'GGCC_C
Cuts at: 0 180 663
Size: 180 483
PstI C_TGCA'G
Cuts at: 0 206 663
Size: 206 457
PvuII CAG'CTG
Cuts at: 0 103 261 408 663
Size: 103 158 147 255
Fragments arranged by size:
255 158 147 103
SanDI GG'GwC_CC
Cuts at: 0 142 663
Size: 142 521
SfcI C'TryA_G
Cuts at: 0 202 274 663
Size: 202 72 389
Fragments arranged by size:
389 202 72
SmaI CCC'GGG
Cuts at: 0 140 663
Size: 140 523
StyI C'CwwG_G
Cuts at: 0 575 663
Size: 575 88
Tth111I GACn'n_nGTC
Cuts at: 0 26 663
Size: 26 637
XmaI C'CCGG_G
Cuts at: 0 138 663
Size: 138 525
Enzymes that do cut:
AarI AccI AclI AcuI AfeI AlwNI ApaI AvaI
BanI BanII BbvCI BglI Bme1580I BpmI Bpu10I BsaBI
BseYI BsgI BsmBI Bsp1286I BspMI BsrBI BstAPI BtsI
DraIII EarI Eco57MI EcoO109I HaeII Hin4I HindIII MmeI
MspA1I PasI PflMI PpuMI PsiI PspOMI PstI PvuII
SanDI SfcI SmaI StyI Tth111I XmaI
Enzymes that do not cut:
AatII Acc65I AflII AflIII AgeI AhdI AleI AlfI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbsI BcgI BciVI BclI BfrBI BglII
BlpI BmgBI BmrI BmtI BplI BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BsiEI BsiHKAI BsiWI BsmI
BspEI BspHI BsrDI BsrFI BsrGI BssHII BssSI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DraI DrdI EaeI EagI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HincII HpaI
KasI KpnI MfeI MluI MscI MslI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PfoI PmeI PmlI PpiI PshAI PspXI
PsrI PvuI RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII TatI
TstI XbaI XcmI XhoI XmnI ZraI