Restriction map for
pF1KB6986
(Linear) (Six-base) MAPSORT of: KIBB6986.seq Check: 4546 from: 1 to: 744
KIBB6986.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 686 744
Size: 361 325 58
Fragments arranged by size:
361 325 58
ApaI G_GGCC'C
Cuts at: 0 261 744
Size: 261 483
AvaI C'yCGr_G
Cuts at: 0 260 447 744
Size: 260 187 297
Fragments arranged by size:
297 260 187
AvrII C'CTAG_G
Cuts at: 0 424 744
Size: 424 320
BanI G'GyrC_C
Cuts at: 0 490 744
Size: 490 254
BanII G_rGCy'C
Cuts at: 0 88 261 744
Size: 88 173 483
Fragments arranged by size:
483 173 88
BbsI GAAGACnn'nnnn_
Cuts at: 0 18 744
Size: 18 726
BclI T'GATC_A
Cuts at: 0 572 744
Size: 572 172
BglI GCCn_nnn'nGGC
Cuts at: 0 469 744
Size: 469 275
BglII A'GATC_T
Cuts at: 0 182 744
Size: 182 562
Bme1580I G_kGCm'C
Cuts at: 0 261 454 744
Size: 261 193 290
Fragments arranged by size:
290 261 193
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 535 744
Size: 535 209
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 634 744
Size: 634 110
BsaBI GATnn'nnATC
Cuts at: 0 247 744
Size: 247 497
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 127 157 744
Size: 127 30 587
Fragments arranged by size:
587 127 30
BsiEI CG_ry'CG
Cuts at: 0 12 92 744
Size: 12 80 652
Fragments arranged by size:
652 80 12
Bsp1286I G_dGCh'C
Cuts at: 0 88 261 454 744
Size: 88 173 193 290
Fragments arranged by size:
290 193 173 88
BspHI T'CATG_A
Cuts at: 0 629 744
Size: 629 115
BsrDI GCAATG_nn'
Cuts at: 0 369 744
Size: 369 375
BsrFI r'CCGG_y
Cuts at: 0 281 744
Size: 281 463
BstXI CCAn_nnnn'nTGG
Cuts at: 0 567 744
Size: 567 177
BstYI r'GATC_y
Cuts at: 0 182 744
Size: 182 562
BtgI C'CryG_G
Cuts at: 0 92 744
Size: 92 652
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 315 744
Size: 315 429
DraI TTT'AAA
Cuts at: 0 659 744
Size: 659 85
EaeI y'GGCC_r
Cuts at: 0 39 149 744
Size: 39 110 595
Fragments arranged by size:
595 110 39
EarI CTCTTCn'nnn_
Cuts at: 0 52 105 744
Size: 52 53 639
Fragments arranged by size:
639 53 52
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 535 686 744
Size: 361 174 151 58
Fragments arranged by size:
361 174 151 58
EcoO109I rG'GnC_Cy
Cuts at: 0 257 297 744
Size: 257 40 447
Fragments arranged by size:
447 257 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 127 159 624 645 656 677 744
Size: 127 32 465 21 11 21 67
Fragments arranged by size:
465 127 67 32 21 21 11
MscI TGG'CCA
Cuts at: 0 151 744
Size: 151 593
MspA1I CmG'CkG
Cuts at: 0 94 744
Size: 94 650
PfoI T'CCnGG_A
Cuts at: 0 512 584 744
Size: 512 72 160
Fragments arranged by size:
512 160 72
PpuMI rG'GwC_Cy
Cuts at: 0 297 744
Size: 297 447
PspOMI G'GGCC_C
Cuts at: 0 257 744
Size: 257 487
PstI C_TGCA'G
Cuts at: 0 161 744
Size: 161 583
SacII CC_GC'GG
Cuts at: 0 95 744
Size: 95 649
SfcI C'TryA_G
Cuts at: 0 157 744
Size: 157 587
SmaI CCC'GGG
Cuts at: 0 262 744
Size: 262 482
SmlI C'TyrA_G
Cuts at: 0 649 744
Size: 649 95
StuI AGG'CCT
Cuts at: 0 224 744
Size: 224 520
StyI C'CwwG_G
Cuts at: 0 424 744
Size: 424 320
TatI w'GTAC_w
Cuts at: 0 314 744
Size: 314 430
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 171 203 744
Size: 171 32 541
Fragments arranged by size:
541 171 32
Tth111I GACn'n_nGTC
Cuts at: 0 202 301 744
Size: 202 99 443
Fragments arranged by size:
443 202 99
XmaI C'CCGG_G
Cuts at: 0 260 744
Size: 260 484
XmnI GAAnn'nnTTC
Cuts at: 0 593 744
Size: 593 151
Enzymes that do cut:
AcuI ApaI AvaI AvrII BanI BanII BbsI BclI
BglI BglII Bme1580I BpmI BpuEI BsaBI BsaXI BsiEI
Bsp1286I BspHI BsrDI BsrFI BstXI BstYI BtgI BtgZI
DraI EaeI EarI Eco57MI EcoO109I Hin4I MscI MspA1I
PfoI PpuMI PspOMI PstI SacII SfcI SmaI SmlI
StuI StyI TatI TstI Tth111I XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AscI AseI AsiSI BaeI BamHI BbeI BbvCI BcgI
BciVI BfrBI BlpI BmgBI BmrI BmtI BplI Bpu10I
BsaI BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI
BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtsI ClaI
CspCI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MslI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI
TaqII XbaI XcmI XhoI ZraI