Restriction map for
pF1KB6981
(Linear) (Six-base) MAPSORT of: KIBB6981.seq Check: 377 from: 1 to: 654
KIBB6981.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AccI GT'mk_AC
Cuts at: 0 368 654
Size: 368 286
AfeI AGC'GCT
Cuts at: 0 253 654
Size: 253 401
AlwNI CAG_nnn'CTG
Cuts at: 0 309 654
Size: 309 345
ApoI r'AATT_y
Cuts at: 0 110 654
Size: 110 544
AvaI C'yCGr_G
Cuts at: 0 345 654
Size: 345 309
BanI G'GyrC_C
Cuts at: 0 289 654
Size: 289 365
BbvCI CC'TCA_GC
Cuts at: 0 45 654
Size: 45 609
BglI GCCn_nnn'nGGC
Cuts at: 0 185 654
Size: 185 469
Bme1580I G_kGCm'C
Cuts at: 0 190 292 654
Size: 190 102 362
Fragments arranged by size:
362 190 102
BmgBI CAC'GTC
Cuts at: 0 366 654
Size: 366 288
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 90 606 654
Size: 90 516 48
Fragments arranged by size:
516 90 48
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 654
Size: 259 395
Bpu10I CC'TnA_GC
Cuts at: 0 45 654
Size: 45 609
BsaI GGTCTCn'nnnn_
Cuts at: 0 73 136 148 654
Size: 73 63 12 506
Fragments arranged by size:
506 73 63 12
BsaHI Gr'CG_yC
Cuts at: 0 638 654
Size: 638 16
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 208 256 654
Size: 208 48 398
Fragments arranged by size:
398 208 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 560 615 654
Size: 560 55 39
Fragments arranged by size:
560 55 39
BsiHKAI G_wGCw'C
Cuts at: 0 401 497 654
Size: 401 96 157
Fragments arranged by size:
401 157 96
BsmI GAATG_Cn'
Cuts at: 0 591 654
Size: 591 63
Bsp1286I G_dGCh'C
Cuts at: 0 190 292 401 497 654
Size: 190 102 109 96 157
Fragments arranged by size:
190 157 109 102 96
BspHI T'CATG_A
Cuts at: 0 482 654
Size: 482 172
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 308 654
Size: 308 346
BssSI C'ACGA_G
Cuts at: 0 520 654
Size: 520 134
BstAPI GCAn_nnn'nTGC
Cuts at: 0 125 654
Size: 125 529
BstBI TT'CG_AA
Cuts at: 0 386 654
Size: 386 268
BstYI r'GATC_y
Cuts at: 0 206 306 422 654
Size: 206 100 116 232
Fragments arranged by size:
232 206 116 100
BtgI C'CryG_G
Cuts at: 0 563 654
Size: 563 91
DraIII CAC_nnn'GTG
Cuts at: 0 167 654
Size: 167 487
EaeI y'GGCC_r
Cuts at: 0 602 647 654
Size: 602 45 7
Fragments arranged by size:
602 45 7
EarI CTCTTCn'nnn_
Cuts at: 0 385 654
Size: 385 269
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 166 654
Size: 166 488
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 654
Size: 259 395
HaeII r_GCGC'y
Cuts at: 0 135 255 654
Size: 135 120 399
Fragments arranged by size:
399 135 120
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 448 480 654
Size: 448 32 174
Fragments arranged by size:
448 174 32
HincII GTy'rAC
Cuts at: 0 509 654
Size: 509 145
MscI TGG'CCA
Cuts at: 0 604 654
Size: 604 50
MslI CAynn'nnrTG
Cuts at: 0 502 654
Size: 502 152
MspA1I CmG'CkG
Cuts at: 0 237 654
Size: 237 417
NdeI CA'TA_TG
Cuts at: 0 514 654
Size: 514 140
PstI C_TGCA'G
Cuts at: 0 305 654
Size: 305 349
PvuII CAG'CTG
Cuts at: 0 237 654
Size: 237 417
SapI GCTCTTCn'nnn_
Cuts at: 0 385 654
Size: 385 269
SbfI CC_TGCA'GG
Cuts at: 0 305 654
Size: 305 349
SfcI C'TryA_G
Cuts at: 0 301 654
Size: 301 353
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 97 654
Size: 97 557
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 456 519 654
Size: 456 63 135
Fragments arranged by size:
456 135 63
Enzymes that do cut:
AccI AfeI AlwNI ApoI AvaI BanI BbvCI BglI
Bme1580I BmgBI BplI BpmI Bpu10I BsaI BsaHI BseRI
BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BssSI BstAPI
BstBI BstYI BtgI DraIII EaeI EarI EciI Eco57MI
HaeII Hin4I HincII MscI MslI MspA1I NdeI PstI
PvuII SapI SbfI SfcI TaqII XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglII BlpI BmrI BmtI BpuEI
BsaAI BsaBI BsaWI BsaXI BseYI BsiEI BsiWI BsmBI
BspEI BsrBI BsrDI BsrFI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI
EagI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI
FspI FspAI HindIII HpaI KasI KpnI MfeI MluI
MmeI NaeI NarI NcoI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI