Restriction map for
pF1KB6973
(Linear) (Six-base) MAPSORT of: KIBB6973.seq Check: 4473 from: 1 to: 651
KIBB6973.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 343 651
Size: 343 308
AlwNI CAG_nnn'CTG
Cuts at: 0 213 651
Size: 213 438
ApaI G_GGCC'C
Cuts at: 0 536 651
Size: 536 115
ApoI r'AATT_y
Cuts at: 0 186 651
Size: 186 465
AvaI C'yCGr_G
Cuts at: 0 412 574 651
Size: 412 162 77
Fragments arranged by size:
412 162 77
BanI G'GyrC_C
Cuts at: 0 633 651
Size: 633 18
BanII G_rGCy'C
Cuts at: 0 536 651
Size: 536 115
BbeI G_GCGC'C
Cuts at: 0 637 651
Size: 637 14
BclI T'GATC_A
Cuts at: 0 86 299 651
Size: 86 213 352
Fragments arranged by size:
352 213 86
BglI GCCn_nnn'nGGC
Cuts at: 0 344 445 651
Size: 344 101 206
Fragments arranged by size:
344 206 101
BglII A'GATC_T
Cuts at: 0 365 651
Size: 365 286
BlpI GC'TnA_GC
Cuts at: 0 457 651
Size: 457 194
Bme1580I G_kGCm'C
Cuts at: 0 536 651
Size: 536 115
BmrI ACTGGGnnnn_n'
Cuts at: 0 295 651
Size: 295 356
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 220 406 651
Size: 97 123 186 245
Fragments arranged by size:
245 186 123 97
BsaHI Gr'CG_yC
Cuts at: 0 481 634 651
Size: 481 153 17
Fragments arranged by size:
481 153 17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 535 651
Size: 535 116
BseYI C'CCAG_C
Cuts at: 0 9 544 651
Size: 9 535 107
Fragments arranged by size:
535 107 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 354 651
Size: 354 297
BsiEI CG_ry'CG
Cuts at: 0 623 651
Size: 623 28
BsmBI CGTCTCn'nnnn_
Cuts at: 0 472 651
Size: 472 179
Bsp1286I G_dGCh'C
Cuts at: 0 536 651
Size: 536 115
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 343 651
Size: 343 308
BsrFI r'CCGG_y
Cuts at: 0 483 651
Size: 483 168
BsrGI T'GTAC_A
Cuts at: 0 305 651
Size: 305 346
BstYI r'GATC_y
Cuts at: 0 365 651
Size: 365 286
BtgI C'CryG_G
Cuts at: 0 68 275 651
Size: 68 207 376
Fragments arranged by size:
376 207 68
DrdI GACnn_nn'nnGTC
Cuts at: 0 136 651
Size: 136 515
EaeI y'GGCC_r
Cuts at: 0 437 560 587 620 651
Size: 437 123 27 33 31
Fragments arranged by size:
437 123 33 31 27
EagI C'GGCC_G
Cuts at: 0 620 651
Size: 620 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 610 651
Size: 610 41
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 220 406 651
Size: 97 123 186 245
Fragments arranged by size:
245 186 123 97
EcoO109I rG'GnC_Cy
Cuts at: 0 446 532 651
Size: 446 86 119
Fragments arranged by size:
446 119 86
HaeII r_GCGC'y
Cuts at: 0 283 420 637 651
Size: 283 137 217 14
Fragments arranged by size:
283 217 137 14
KasI G'GCGC_C
Cuts at: 0 633 651
Size: 633 18
MslI CAynn'nnrTG
Cuts at: 0 403 651
Size: 403 248
MspA1I CmG'CkG
Cuts at: 0 165 378 456 651
Size: 165 213 78 195
Fragments arranged by size:
213 195 165 78
NaeI GCC'GGC
Cuts at: 0 485 651
Size: 485 166
NarI GG'CG_CC
Cuts at: 0 634 651
Size: 634 17
NcoI C'CATG_G
Cuts at: 0 68 275 651
Size: 68 207 376
Fragments arranged by size:
376 207 68
NgoMIV G'CCGG_C
Cuts at: 0 483 651
Size: 483 168
PasI CC'CwG_GG
Cuts at: 0 46 651
Size: 46 605
PspOMI G'GGCC_C
Cuts at: 0 532 651
Size: 532 119
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 313 345 651
Size: 313 32 306
Fragments arranged by size:
313 306 32
PstI C_TGCA'G
Cuts at: 0 19 223 651
Size: 19 204 428
Fragments arranged by size:
428 204 19
PvuII CAG'CTG
Cuts at: 0 165 651
Size: 165 486
SexAI A'CCwGG_T
Cuts at: 0 131 651
Size: 131 520
SfcI C'TryA_G
Cuts at: 0 15 219 651
Size: 15 204 432
Fragments arranged by size:
432 204 15
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 445 651
Size: 445 206
SfoI GGC'GCC
Cuts at: 0 635 651
Size: 635 16
SgrAI Cr'CCGG_yG
Cuts at: 0 483 651
Size: 483 168
SmaI CCC'GGG
Cuts at: 0 576 651
Size: 576 75
StyI C'CwwG_G
Cuts at: 0 68 95 275 515 590 651
Size: 68 27 180 240 75 61
Fragments arranged by size:
240 180 75 68 61 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 242 553 651
Size: 242 311 98
Fragments arranged by size:
311 242 98
TatI w'GTAC_w
Cuts at: 0 305 392 651
Size: 305 87 259
Fragments arranged by size:
305 259 87
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 282 651
Size: 282 369
XmaI C'CCGG_G
Cuts at: 0 574 651
Size: 574 77
Enzymes that do cut:
AarI AlwNI ApaI ApoI AvaI BanI BanII BbeI
BclI BglI BglII BlpI Bme1580I BmrI BpmI BsaHI
BseRI BseYI BsgI BsiEI BsmBI Bsp1286I BspMI BsrFI
BsrGI BstYI BtgI DrdI EaeI EagI EciI Eco57MI
EcoO109I HaeII KasI MslI MspA1I NaeI NarI NcoI
NgoMIV PasI PspOMI PsrI PstI PvuII SexAI SfcI
SfiI SfoI SgrAI SmaI StyI TaqII TatI XcmI
XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI
BfrBI BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaWI BsaXI BsiHKAI BsiWI BsmI BspEI BspHI
BsrBI BsrDI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII
EarI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI NdeI NheI NotI NruI NsiI NspI
PacI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SgfI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TstI Tth111I XbaI
XhoI XmnI ZraI