Restriction map for
pF1KB6966
(Linear) (Six-base) MAPSORT of: KIBB6966.seq Check: 4492 from: 1 to: 723
KIBB6966.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
Acc65I G'GTAC_C
Cuts at: 0 275 723
Size: 275 448
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 233 430 491 723
Size: 26 207 197 61 232
Fragments arranged by size:
232 207 197 61 26
AleI CACnn'nnGTG
Cuts at: 0 214 723
Size: 214 509
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 232 279 556 723
Size: 232 47 277 167
Fragments arranged by size:
277 232 167 47
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 289 321 723
Size: 289 32 402
Fragments arranged by size:
402 289 32
AlwNI CAG_nnn'CTG
Cuts at: 0 87 171 723
Size: 87 84 552
Fragments arranged by size:
552 87 84
ApaLI G'TGCA_C
Cuts at: 0 217 723
Size: 217 506
ApoI r'AATT_y
Cuts at: 0 244 337 357 652 723
Size: 244 93 20 295 71
Fragments arranged by size:
295 244 93 71 20
AvaI C'yCGr_G
Cuts at: 0 598 644 723
Size: 598 46 79
Fragments arranged by size:
598 79 46
BanI G'GyrC_C
Cuts at: 0 275 723
Size: 275 448
BanII G_rGCy'C
Cuts at: 0 518 692 723
Size: 518 174 31
Fragments arranged by size:
518 174 31
Bme1580I G_kGCm'C
Cuts at: 0 178 221 268 723
Size: 178 43 47 455
Fragments arranged by size:
455 178 47 43
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 406 723
Size: 271 135 317
Fragments arranged by size:
317 271 135
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 648 723
Size: 91 557 75
Fragments arranged by size:
557 91 75
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 694 723
Size: 694 29
BseYI C'CCAG_C
Cuts at: 0 316 366 723
Size: 316 50 357
Fragments arranged by size:
357 316 50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 723
Size: 184 539
BsiHKAI G_wGCw'C
Cuts at: 0 221 518 692 723
Size: 221 297 174 31
Fragments arranged by size:
297 221 174 31
Bsp1286I G_dGCh'C
Cuts at: 0 178 221 268 518 692 723
Size: 178 43 47 250 174 31
Fragments arranged by size:
250 178 174 47 43 31
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 9 723
Size: 9 714
BsrDI GCAATG_nn'
Cuts at: 0 639 723
Size: 639 84
BtsI GCAGTG_nn'
Cuts at: 0 51 263 723
Size: 51 212 460
Fragments arranged by size:
460 212 51
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 165 723
Size: 165 558
EcoICRI GAG'CTC
Cuts at: 0 516 690 723
Size: 516 174 33
Fragments arranged by size:
516 174 33
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 233 271 406 430 491 723
Size: 26 207 38 135 24 61 232
Fragments arranged by size:
232 207 135 61 38 26 24
EcoO109I rG'GnC_Cy
Cuts at: 0 714 723
Size: 714 9
EcoRI G'AATT_C
Cuts at: 0 357 652 723
Size: 357 295 71
Fragments arranged by size:
357 295 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 578 610 723
Size: 578 32 113
Fragments arranged by size:
578 113 32
HincII GTy'rAC
Cuts at: 0 138 723
Size: 138 585
KpnI G_GTAC'C
Cuts at: 0 279 723
Size: 279 444
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 332 723
Size: 332 391
MslI CAynn'nnrTG
Cuts at: 0 214 723
Size: 214 509
MspA1I CmG'CkG
Cuts at: 0 316 723
Size: 316 407
PasI CC'CwG_GG
Cuts at: 0 178 723
Size: 178 545
PvuII CAG'CTG
Cuts at: 0 316 723
Size: 316 407
RsrII CG'GwC_CG
Cuts at: 0 152 723
Size: 152 571
SacI G_AGCT'C
Cuts at: 0 518 692 723
Size: 518 174 31
Fragments arranged by size:
518 174 31
SmaI CCC'GGG
Cuts at: 0 646 723
Size: 646 77
SmlI C'TyrA_G
Cuts at: 0 106 627 723
Size: 106 521 96
Fragments arranged by size:
521 106 96
StuI AGG'CCT
Cuts at: 0 604 723
Size: 604 119
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 169 723
Size: 169 554
TatI w'GTAC_w
Cuts at: 0 558 723
Size: 558 165
XmaI C'CCGG_G
Cuts at: 0 644 723
Size: 644 79
Enzymes that do cut:
Acc65I AcuI AleI AlfI AloI AlwNI ApaLI ApoI
AvaI BanI BanII Bme1580I BpmI BpuEI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspMI BsrDI BtsI EciI EcoICRI
Eco57MI EcoO109I EcoRI Hin4I HincII KpnI MmeI MslI
MspA1I PasI PvuII RsrII SacI SmaI SmlI StuI
TaqII TatI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI ApaI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EcoNI EcoRV FalI FseI FspI FspAI HaeII
HindIII HpaI KasI MfeI MluI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StyI SwaI TstI Tth111I XbaI XcmI XhoI XmnI
ZraI