(Linear) (Six-base) MAPSORT of: KIBB6963.seq  Check: 6992  from: 1  to: 642



KIBB6963.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:07  ..

AccI GT'mk_AC

Cuts at:      0      61     642 
   Size:         61     581
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      33     642 
   Size:         33     609
 
AflII C'TTAA_G

Cuts at:      0     501     642 
   Size:        501     141
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     455     642 
   Size:        455     187
 
BanI G'GyrC_C

Cuts at:      0     154     642 
   Size:        154     488
 
BanII G_rGCy'C

Cuts at:      0      57     642 
   Size:         57     585
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      39     638     642 
   Size:         39     599       4

  Fragments arranged by size: 

                 599      39       4
 
BglI GCCn_nnn'nGGC

Cuts at:      0     347     642 
   Size:        347     295
 
BlpI GC'TnA_GC

Cuts at:      0     200     642 
   Size:        200     442
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     342     642 
   Size:        342     300
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      65     642 
   Size:         65     577
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     276     642 
   Size:        276     366
 
Bsp1286I G_dGCh'C

Cuts at:      0      57     642 
   Size:         57     585
 
BsrDI GCAATG_nn'

Cuts at:      0     226     612     642 
   Size:        226     386      30

  Fragments arranged by size: 

                 386     226      30
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     487     642 
   Size:        487     155
 
BstBI TT'CG_AA

Cuts at:      0     594     642 
   Size:        594      48
 
BstZ17I GTA'TAC

Cuts at:      0      62     642 
   Size:         62     580
 
BtgI C'CryG_G

Cuts at:      0     342     642 
   Size:        342     300
 
BtsI GCAGTG_nn'

Cuts at:      0     320     642 
   Size:        320     322
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      33      65     642 
   Size:         33      32     577

  Fragments arranged by size: 

                 577      33      32
 
EcoRV GAT'ATC

Cuts at:      0     462     642 
   Size:        462     180
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     543     642 
   Size:        543      99
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     619     642 
   Size:        619      23
 
MfeI C'AATT_G

Cuts at:      0     141     642 
   Size:        141     501
 
NspI r_CATG'y

Cuts at:      0     436     642 
   Size:        436     206
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     146     642 
   Size:        146     496
 
SmlI C'TyrA_G

Cuts at:      0     255     501     642 
   Size:        255     246     141

  Fragments arranged by size: 

                 255     246     141
 
SphI G_CATG'C

Cuts at:      0     436     642 
   Size:        436     206
 
SspI AAT'ATT

Cuts at:      0     390     642 
   Size:        390     252
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      30     280     642 
   Size:         30     250     362

  Fragments arranged by size: 

                 362     250      30
 

 Enzymes that do cut:

     AccI     AcuI    AflII     AlfI     BanI    BanII     BbsI     BglI
     BlpI     BmrI     BpmI    BpuEI Bsp1286I    BsrDI   BstAPI    BstBI
  BstZ17I     BtgI     BtsI  Eco57MI    EcoRV     FalI    Hin4I     MfeI
     NspI    PflMI     SmlI     SphI     SspI    TaqII

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI   AflIII     AgeI     AhdI
     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI    BglII Bme1580I    BmgBI     BmtI     BplI
   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstEII
    BstXI    BstYI   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SrfI     StuI     StyI     SwaI     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI