(Linear) (Six-base) MAPSORT of: KIBB6955.seq  Check: 7710  from: 1  to: 636



KIBB6955.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:23  ..

AccI GT'mk_AC

Cuts at:      0     213     636 
   Size:        213     423
 
AlwNI CAG_nnn'CTG

Cuts at:      0     356     636 
   Size:        356     280
 
ApoI r'AATT_y

Cuts at:      0     154     636 
   Size:        154     482
 
BamHI G'GATC_C

Cuts at:      0     526     636 
   Size:        526     110
 
BglII A'GATC_T

Cuts at:      0     436     622     636 
   Size:        436     186      14

  Fragments arranged by size: 

                 436     186      14
 
BmrI ACTGGGnnnn_n'

Cuts at:      0       5     636 
   Size:          5     631
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      54     636 
   Size:         54     582
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     509     636 
   Size:        509     127
 
BsaBI GATnn'nnATC

Cuts at:      0     627     636 
   Size:        627       9
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     426     456     636 
   Size:        426      30     180

  Fragments arranged by size: 

                 426     180      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     219     575     636 
   Size:        219     356      61

  Fragments arranged by size: 

                 356     219      61
 
BsiHKAI G_wGCw'C

Cuts at:      0     141     636 
   Size:        141     495
 
Bsp1286I G_dGCh'C

Cuts at:      0     141     636 
   Size:        141     495
 
BspHI T'CATG_A

Cuts at:      0     567     636 
   Size:        567      69
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     391     636 
   Size:        391     245
 
BsrDI GCAATG_nn'

Cuts at:      0     298     636 
   Size:        298     338
 
BssSI C'ACGA_G

Cuts at:      0     104     636 
   Size:        104     532
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     295     636 
   Size:        295     341
 
BstYI r'GATC_y

Cuts at:      0     279     436     526     622     636 
   Size:        279     157      90      96      14

  Fragments arranged by size: 

                 279     157      96      90      14
 
BstZ17I GTA'TAC

Cuts at:      0     214     636 
   Size:        214     422
 
BtsI GCAGTG_nn'

Cuts at:      0     414     636 
   Size:        414     222
 
DraI TTT'AAA

Cuts at:      0     159     636 
   Size:        159     477
 
EarI CTCTTCn'nnn_

Cuts at:      0      44     146     365     636 
   Size:         44     102     219     271

  Fragments arranged by size: 

                 271     219     102      44
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     426     458     469     501     601     633     636 
   Size:        426      32      11      32     100      32       3

  Fragments arranged by size: 

                 426     100      32      32      32      11       3
 
MslI CAynn'nnrTG

Cuts at:      0     419     636 
   Size:        419     217
 
MspA1I CmG'CkG

Cuts at:      0     353     636 
   Size:        353     283
 
PvuII CAG'CTG

Cuts at:      0     353     636 
   Size:        353     283
 
SapI GCTCTTCn'nnn_

Cuts at:      0      44     146     365     636 
   Size:         44     102     219     271

  Fragments arranged by size: 

                 271     219     102      44
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     322     636 
   Size:        322     314
 

 Enzymes that do cut:

     AccI    AlwNI     ApoI    BamHI    BglII     BmrI     BplI     BsaI
    BsaBI    BsaXI     BsgI  BsiHKAI Bsp1286I    BspHI    BspMI    BsrDI
    BssSI    BstXI    BstYI  BstZ17I     BtsI     DraI     EarI    Hin4I
     MslI   MspA1I    PvuII     SapI    TaqII

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI
 Bme1580I    BmgBI     BmtI     BpmI   Bpu10I    BpuEI    BsaAI    BsaHI
    BsaWI    BseRI    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BsrBI    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII   Bsu36I
     BtgI    BtgZI     ClaI    CspCI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI