(Linear) (Six-base) MAPSORT of: KIBB6948.seq  Check: 8522  from: 1  to: 645



KIBB6948.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:07  ..

ApoI r'AATT_y

Cuts at:      0     436     645 
   Size:        436     209
 
BamHI G'GATC_C

Cuts at:      0     221     645 
   Size:        221     424
 
BmgBI CAC'GTC

Cuts at:      0     286     645 
   Size:        286     359
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     597     645 
   Size:        597      48
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     185     384     645 
   Size:        185     199     261

  Fragments arranged by size: 

                 261     199     185
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     322     645 
   Size:        322     323
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     590     645 
   Size:        590      55
 
BstBI TT'CG_AA

Cuts at:      0     391     645 
   Size:        391     254
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     464     645 
   Size:        464     181
 
BstYI r'GATC_y

Cuts at:      0     221     610     645 
   Size:        221     389      35

  Fragments arranged by size: 

                 389     221      35
 
Bsu36I CC'TnA_GG

Cuts at:      0     305     645 
   Size:        305     340
 
BtgI C'CryG_G

Cuts at:      0     499     645 
   Size:        499     146
 
BtsI GCAGTG_nn'

Cuts at:      0     177     578     645 
   Size:        177     401      67

  Fragments arranged by size: 

                 401     177      67
 
EaeI y'GGCC_r

Cuts at:      0     294     645 
   Size:        294     351
 
EarI CTCTTCn'nnn_

Cuts at:      0     362     645 
   Size:        362     283
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     185     384     645 
   Size:        185     199     261

  Fragments arranged by size: 

                 261     199     185
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     377     645 
   Size:        377     268
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     178     210     645 
   Size:        178      32     435

  Fragments arranged by size: 

                 435     178      32
 
HindIII A'AGCT_T

Cuts at:      0     140     640     645 
   Size:        140     500       5

  Fragments arranged by size: 

                 500     140       5
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     350     645 
   Size:        350     295
 
MscI TGG'CCA

Cuts at:      0     296     645 
   Size:        296     349
 
MslI CAynn'nnrTG

Cuts at:      0     462     645 
   Size:        462     183
 
NcoI C'CATG_G

Cuts at:      0     499     645 
   Size:        499     146
 
SfcI C'TryA_G

Cuts at:      0     413     645 
   Size:        413     232
 
StyI C'CwwG_G

Cuts at:      0       9     499     645 
   Size:          9     490     146

  Fragments arranged by size: 

                 490     146       9
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     492     645 
   Size:        492     153
 
TatI w'GTAC_w

Cuts at:      0     385     645 
   Size:        385     260
 

 Enzymes that do cut:

     ApoI    BamHI    BmgBI     BmrI     BpmI    BseRI     BsgI    BstBI
    BstXI    BstYI   Bsu36I     BtgI     BtsI     EaeI     EarI  Eco57MI
     FalI    Hin4I  HindIII     MmeI     MscI     MslI     NcoI     SfcI
     StyI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I     BmtI     BplI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI   BstEII
  BstZ17I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI
    FspAI    HaeII   HincII     HpaI     KasI     KpnI     MfeI     MluI
   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI