Restriction map for
pF1KB6944
(Linear) (Six-base) MAPSORT of: KIBB6944.seq Check: 4233 from: 1 to: 693
KIBB6944.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 324 693
Size: 324 369
AflIII A'CryG_T
Cuts at: 0 566 693
Size: 566 127
AleI CACnn'nnGTG
Cuts at: 0 37 693
Size: 37 656
ApaI G_GGCC'C
Cuts at: 0 507 693
Size: 507 186
ApaLI G'TGCA_C
Cuts at: 0 535 693
Size: 535 158
AvaI C'yCGr_G
Cuts at: 0 392 500 693
Size: 392 108 193
Fragments arranged by size:
392 193 108
BanI G'GyrC_C
Cuts at: 0 304 547 693
Size: 304 243 146
Fragments arranged by size:
304 243 146
BanII G_rGCy'C
Cuts at: 0 182 507 621 686 693
Size: 182 325 114 65 7
Fragments arranged by size:
325 182 114 65 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 117 693
Size: 117 576
BglI GCCn_nnn'nGGC
Cuts at: 0 596 693
Size: 596 97
BglII A'GATC_T
Cuts at: 0 167 693
Size: 167 526
Bme1580I G_kGCm'C
Cuts at: 0 307 507 539 693
Size: 307 200 32 154
Fragments arranged by size:
307 200 154 32
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 201 693
Size: 201 492
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 693
Size: 271 422
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 25 179 693
Size: 25 154 514
Fragments arranged by size:
514 154 25
BseYI C'CCAG_C
Cuts at: 0 18 405 693
Size: 18 387 288
Fragments arranged by size:
387 288 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 607 693
Size: 61 546 86
Fragments arranged by size:
546 86 61
BsiHKAI G_wGCw'C
Cuts at: 0 182 539 672 693
Size: 182 357 133 21
Fragments arranged by size:
357 182 133 21
Bsp1286I G_dGCh'C
Cuts at: 0 182 307 507 539 621 672 686 693
Size: 182 125 200 32 82 51 14 7
Fragments arranged by size:
200 182 125 82 51 32 14 7
BspHI T'CATG_A
Cuts at: 0 561 693
Size: 561 132
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 324 587 693
Size: 324 263 106
Fragments arranged by size:
324 263 106
BsrFI r'CCGG_y
Cuts at: 0 36 693
Size: 36 657
BstAPI GCAn_nnn'nTGC
Cuts at: 0 630 693
Size: 630 63
BstXI CCAn_nnnn'nTGG
Cuts at: 0 288 693
Size: 288 405
BstYI r'GATC_y
Cuts at: 0 167 693
Size: 167 526
BtgI C'CryG_G
Cuts at: 0 315 693
Size: 315 378
DrdI GACnn_nn'nnGTC
Cuts at: 0 142 693
Size: 142 551
EaeI y'GGCC_r
Cuts at: 0 492 693
Size: 492 201
EarI CTCTTCn'nnn_
Cuts at: 0 187 693
Size: 187 506
EcoICRI GAG'CTC
Cuts at: 0 180 693
Size: 180 513
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 693
Size: 271 422
HaeII r_GCGC'y
Cuts at: 0 66 693
Size: 66 627
MscI TGG'CCA
Cuts at: 0 494 693
Size: 494 199
MslI CAynn'nnrTG
Cuts at: 0 37 542 693
Size: 37 505 151
Fragments arranged by size:
505 151 37
NspI r_CATG'y
Cuts at: 0 199 238 570 693
Size: 199 39 332 123
Fragments arranged by size:
332 199 123 39
PciI A'CATG_T
Cuts at: 0 566 693
Size: 566 127
PspOMI G'GGCC_C
Cuts at: 0 503 693
Size: 503 190
SacI G_AGCT'C
Cuts at: 0 182 693
Size: 182 511
SapI GCTCTTCn'nnn_
Cuts at: 0 187 693
Size: 187 506
SgrAI Cr'CCGG_yG
Cuts at: 0 36 693
Size: 36 657
SmaI CCC'GGG
Cuts at: 0 394 693
Size: 394 299
SphI G_CATG'C
Cuts at: 0 199 693
Size: 199 494
StyI C'CwwG_G
Cuts at: 0 140 663 693
Size: 140 523 30
Fragments arranged by size:
523 140 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 288 693
Size: 288 405
TatI w'GTAC_w
Cuts at: 0 237 693
Size: 237 456
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 288 363 693
Size: 288 75 330
Fragments arranged by size:
330 288 75
XmaI C'CCGG_G
Cuts at: 0 392 693
Size: 392 301
Enzymes that do cut:
AarI AflIII AleI ApaI ApaLI AvaI BanI BanII
BbsI BglI BglII Bme1580I BplI BpmI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspHI BspMI BsrFI BstAPI BstXI
BstYI BtgI DrdI EaeI EarI EcoICRI Eco57MI HaeII
MscI MslI NspI PciI PspOMI SacI SapI SgrAI
SmaI SphI StyI TaqII TatI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AlfI AloI AlwNI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI
BfrBI BlpI BmgBI BmrI BmtI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmI
BsmBI BspEI BsrBI BsrDI BsrGI BssHII BssSI BstBI
BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII EagI EciI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PstI PvuI PvuII RsrII SacII SalI
SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SmlI SnaBI SpeI SrfI SspI StuI SwaI TstI
Tth111I XbaI XhoI XmnI ZraI