Restriction map for
pF1KB6939
(Linear) (Six-base) MAPSORT of: KIBB6939.seq Check: 8004 from: 1 to: 624
KIBB6939.orf
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AatII G_ACGT'C
Cuts at: 0 421 624
Size: 421 203
AfeI AGC'GCT
Cuts at: 0 100 463 624
Size: 100 363 161
Fragments arranged by size:
363 161 100
AflIII A'CryG_T
Cuts at: 0 371 624
Size: 371 253
AgeI A'CCGG_T
Cuts at: 0 568 624
Size: 568 56
ApaI G_GGCC'C
Cuts at: 0 329 624
Size: 329 295
AvaI C'yCGr_G
Cuts at: 0 121 287 624
Size: 121 166 337
Fragments arranged by size:
337 166 121
BanI G'GyrC_C
Cuts at: 0 437 624
Size: 437 187
BanII G_rGCy'C
Cuts at: 0 58 220 329 368 624
Size: 58 162 109 39 256
Fragments arranged by size:
256 162 109 58 39
BbsI GAAGACnn'nnnn_
Cuts at: 0 478 624
Size: 478 146
Bme1580I G_kGCm'C
Cuts at: 0 329 379 494 624
Size: 329 50 115 130
Fragments arranged by size:
329 130 115 50
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 324 624
Size: 324 300
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 624
Size: 73 551
BsaAI yAC'GTr
Cuts at: 0 374 624
Size: 374 250
BsaHI Gr'CG_yC
Cuts at: 0 118 418 624
Size: 118 300 206
Fragments arranged by size:
300 206 118
BsaWI w'CCGG_w
Cuts at: 0 568 624
Size: 568 56
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 310 624
Size: 310 314
BseYI C'CCAG_C
Cuts at: 0 328 624
Size: 328 296
BsiHKAI G_wGCw'C
Cuts at: 0 58 503 624
Size: 58 445 121
Fragments arranged by size:
445 121 58
BsmBI CGTCTCn'nnnn_
Cuts at: 0 425 624
Size: 425 199
Bsp1286I G_dGCh'C
Cuts at: 0 58 220 329 368 379 494 503 624
Size: 58 162 109 39 11 115 9 121
Fragments arranged by size:
162 121 115 109 58 39 11 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 246 624
Size: 246 378
BsrBI CCG'CTC
Cuts at: 0 300 311 624
Size: 300 11 313
Fragments arranged by size:
313 300 11
BsrFI r'CCGG_y
Cuts at: 0 262 440 568 624
Size: 262 178 128 56
Fragments arranged by size:
262 178 128 56
BstEII G'GTnAC_C
Cuts at: 0 258 624
Size: 258 366
BtgI C'CryG_G
Cuts at: 0 281 624
Size: 281 343
EaeI y'GGCC_r
Cuts at: 0 507 624
Size: 507 117
EarI CTCTTCn'nnn_
Cuts at: 0 550 624
Size: 550 74
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 259 624
Size: 259 365
EcoICRI GAG'CTC
Cuts at: 0 56 624
Size: 56 568
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 324 624
Size: 324 300
EcoNI CCTnn'n_nnAGG
Cuts at: 0 64 244 624
Size: 64 180 380
Fragments arranged by size:
380 180 64
EcoO109I rG'GnC_Cy
Cuts at: 0 276 325 600 624
Size: 276 49 275 24
Fragments arranged by size:
276 275 49 24
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 417 624
Size: 417 207
HaeII r_GCGC'y
Cuts at: 0 102 465 624
Size: 102 363 159
Fragments arranged by size:
363 159 102
MspA1I CmG'CkG
Cuts at: 0 283 624
Size: 283 341
PasI CC'CwG_GG
Cuts at: 0 60 624
Size: 60 564
PmlI CAC'GTG
Cuts at: 0 374 624
Size: 374 250
PpuMI rG'GwC_Cy
Cuts at: 0 276 600 624
Size: 276 324 24
Fragments arranged by size:
324 276 24
PspOMI G'GGCC_C
Cuts at: 0 325 624
Size: 325 299
PstI C_TGCA'G
Cuts at: 0 106 388 624
Size: 106 282 236
Fragments arranged by size:
282 236 106
SacI G_AGCT'C
Cuts at: 0 58 624
Size: 58 566
SacII CC_GC'GG
Cuts at: 0 284 624
Size: 284 340
SanDI GG'GwC_CC
Cuts at: 0 600 624
Size: 600 24
SfcI C'TryA_G
Cuts at: 0 102 384 624
Size: 102 282 240
Fragments arranged by size:
282 240 102
SgrAI Cr'CCGG_yG
Cuts at: 0 568 624
Size: 568 56
SmaI CCC'GGG
Cuts at: 0 123 624
Size: 123 501
SmlI C'TyrA_G
Cuts at: 0 88 624
Size: 88 536
StyI C'CwwG_G
Cuts at: 0 554 624
Size: 554 70
TatI w'GTAC_w
Cuts at: 0 35 398 624
Size: 35 363 226
Fragments arranged by size:
363 226 35
XmaI C'CCGG_G
Cuts at: 0 121 624
Size: 121 503
XmnI GAAnn'nnTTC
Cuts at: 0 151 624
Size: 151 473
ZraI GAC'GTC
Cuts at: 0 419 624
Size: 419 205
Enzymes that do cut:
AatII AfeI AflIII AgeI ApaI AvaI BanI BanII
BbsI Bme1580I BpmI BpuEI BsaAI BsaHI BsaWI BseRI
BseYI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BstEII
BtgI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI HaeII MspA1I PasI PmlI PpuMI PspOMI PstI
SacI SacII SanDI SfcI SgrAI SmaI SmlI StyI
TatI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AflII AhdI AleI
AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI
Bpu10I BsaI BsaBI BsaXI BsgI BsiEI BsiWI BsmI
BspEI BspHI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstXI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EagI EcoRI EcoRV FseI FspI
FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspXI PsrI
PvuI PvuII RsrII SalI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TstI Tth111I XbaI XcmI XhoI