(Linear) (Six-base) MAPSORT of: KIBB6918.seq  Check: 6629  from: 1  to: 675



KIBB6918.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

Acc65I G'GTAC_C

Cuts at:      0     546     675 
   Size:        546     129
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     581     675 
   Size:        581      94
 
ApoI r'AATT_y

Cuts at:      0     387     408     577     675 
   Size:        387      21     169      98

  Fragments arranged by size: 

                 387     169      98      21
 
BanI G'GyrC_C

Cuts at:      0     546     675 
   Size:        546     129
 
BanII G_rGCy'C

Cuts at:      0     181     675 
   Size:        181     494
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     184     675 
   Size:        184     491
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     620     675 
   Size:        620      55
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     216     675 
   Size:        216     459
 
Bsp1286I G_dGCh'C

Cuts at:      0     181     675 
   Size:        181     494
 
BsrDI GCAATG_nn'

Cuts at:      0     563     675 
   Size:        563     112
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     336     675 
   Size:        336     339
 
BstYI r'GATC_y

Cuts at:      0      73     675 
   Size:         73     602
 
Bsu36I CC'TnA_GG

Cuts at:      0     425     675 
   Size:        425     250
 
DraI TTT'AAA

Cuts at:      0     128     675 
   Size:        128     547
 
EaeI y'GGCC_r

Cuts at:      0     372     675 
   Size:        372     303
 
EarI CTCTTCn'nnn_

Cuts at:      0      64     473     675 
   Size:         64     409     202

  Fragments arranged by size: 

                 409     202      64
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     620     675 
   Size:        620      55
 
EcoO109I rG'GnC_Cy

Cuts at:      0     429     675 
   Size:        429     246
 
EcoRV GAT'ATC

Cuts at:      0       6     675 
   Size:          6     669
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     256     288     617     649     675 
   Size:        256      32     329      32      26

  Fragments arranged by size: 

                 329     256      32      32      26
 
KpnI G_GTAC'C

Cuts at:      0     550     675 
   Size:        550     125
 
MscI TGG'CCA

Cuts at:      0     374     675 
   Size:        374     301
 
MslI CAynn'nnrTG

Cuts at:      0     139     675 
   Size:        139     536
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     223     675 
   Size:        223     452
 
PpuMI rG'GwC_Cy

Cuts at:      0     429     675 
   Size:        429     246
 
SmlI C'TyrA_G

Cuts at:      0     195     675 
   Size:        195     480
 
StuI AGG'CCT

Cuts at:      0     154     675 
   Size:        154     521
 
StyI C'CwwG_G

Cuts at:      0     402     675 
   Size:        402     273
 

 Enzymes that do cut:

   Acc65I     AlfI     ApoI     BanI    BanII     BmrI     BpmI    BpuEI
 Bsp1286I    BsrDI    BstXI    BstYI   Bsu36I     DraI     EaeI     EarI
  Eco57MI EcoO109I    EcoRV    Hin4I     KpnI     MscI     MslI    PflMI
    PpuMI     SmlI     StuI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I     BtgI
    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI     EagI     EciI
  EcoICRI    EcoNI    EcoRI     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     MfeI     MluI     MmeI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI