(Linear) (Six-base) MAPSORT of: KIBB6916.seq  Check: 2727  from: 1  to: 675



KIBB6916.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AccI GT'mk_AC

Cuts at:      0     410     675 
   Size:        410     265
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     442     675 
   Size:        442     233
 
AfeI AGC'GCT

Cuts at:      0     101     675 
   Size:        101     574
 
ApoI r'AATT_y

Cuts at:      0     205     675 
   Size:        205     470
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     567     675 
   Size:        567     108
 
BclI T'GATC_A

Cuts at:      0      57     675 
   Size:         57     618
 
Bme1580I G_kGCm'C

Cuts at:      0      10     675 
   Size:         10     665
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     330     675 
   Size:        330     345
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      44     675 
   Size:         44     631
 
Bpu10I CC'TnA_GC

Cuts at:      0     148     675 
   Size:        148     527
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      17      47     675 
   Size:         17      30     628

  Fragments arranged by size: 

                 628      30      17
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     124     267     675 
   Size:        124     143     408

  Fragments arranged by size: 

                 408     143     124
 
Bsp1286I G_dGCh'C

Cuts at:      0      10     675 
   Size:         10     665
 
BstYI r'GATC_y

Cuts at:      0     523     675 
   Size:        523     152
 
BstZ17I GTA'TAC

Cuts at:      0     411     675 
   Size:        411     264
 
DraI TTT'AAA

Cuts at:      0     488     675 
   Size:        488     187
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      44     442     675 
   Size:         44     398     233

  Fragments arranged by size: 

                 398     233      44
 
HaeII r_GCGC'y

Cuts at:      0     103     675 
   Size:        103     572
 
MfeI C'AATT_G

Cuts at:      0     233     675 
   Size:        233     442
 
MspA1I CmG'CkG

Cuts at:      0     520     675 
   Size:        520     155
 
NdeI CA'TA_TG

Cuts at:      0     431     675 
   Size:        431     244
 
NspI r_CATG'y

Cuts at:      0     510     675 
   Size:        510     165
 
PstI C_TGCA'G

Cuts at:      0      99     675 
   Size:         99     576
 
PvuII CAG'CTG

Cuts at:      0     520     675 
   Size:        520     155
 
SfcI C'TryA_G

Cuts at:      0      95     675 
   Size:         95     580
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      17      49     675 
   Size:         17      32     626

  Fragments arranged by size: 

                 626      32      17
 

 Enzymes that do cut:

     AccI     AcuI     AfeI     ApoI    BciVI     BclI Bme1580I     BmrI
     BpmI   Bpu10I    BsaXI    BseRI Bsp1286I    BstYI  BstZ17I     DraI
  Eco57MI    HaeII     MfeI   MspA1I     NdeI     NspI     PstI    PvuII
     SfcI     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MluI
     MmeI     MscI     MslI     NaeI     NarI     NcoI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI