Restriction map for
pF1KB6912
(Linear) (Six-base) MAPSORT of: KIBB6912.seq Check: 2102 from: 1 to: 654
KIBB6912.orf
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AatII G_ACGT'C
Cuts at: 0 173 654
Size: 173 481
AflIII A'CryG_T
Cuts at: 0 396 654
Size: 396 258
AleI CACnn'nnGTG
Cuts at: 0 295 654
Size: 295 359
BanI G'GyrC_C
Cuts at: 0 284 423 465 643 654
Size: 284 139 42 178 11
Fragments arranged by size:
284 178 139 42 11
BanII G_rGCy'C
Cuts at: 0 566 654
Size: 566 88
BbeI G_GCGC'C
Cuts at: 0 288 469 654
Size: 288 181 185
Fragments arranged by size:
288 185 181
BbsI GAAGACnn'nnnn_
Cuts at: 0 373 479 629 654
Size: 373 106 150 25
Fragments arranged by size:
373 150 106 25
BciVI GTATCCnnnnn_n'
Cuts at: 0 578 654
Size: 578 76
Bme1580I G_kGCm'C
Cuts at: 0 428 646 654
Size: 428 218 8
Fragments arranged by size:
428 218 8
BmgBI CAC'GTC
Cuts at: 0 399 654
Size: 399 255
BsaI GGTCTCn'nnnn_
Cuts at: 0 109 654
Size: 109 545
BsaHI Gr'CG_yC
Cuts at: 0 141 170 285 466 654
Size: 141 29 115 181 188
Fragments arranged by size:
188 181 141 115 29
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 451 454 496 574 654
Size: 451 3 42 78 80
Fragments arranged by size:
451 80 78 42 3
BseYI C'CCAG_C
Cuts at: 0 92 654
Size: 92 562
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 598 654
Size: 598 56
BsiEI CG_ry'CG
Cuts at: 0 130 207 654
Size: 130 77 447
Fragments arranged by size:
447 130 77
Bsp1286I G_dGCh'C
Cuts at: 0 428 566 646 654
Size: 428 138 80 8
Fragments arranged by size:
428 138 80 8
BsrBI CCG'CTC
Cuts at: 0 216 654
Size: 216 438
BsrFI r'CCGG_y
Cuts at: 0 143 150 462 654
Size: 143 7 312 192
Fragments arranged by size:
312 192 143 7
BssHII G'CGCG_C
Cuts at: 0 54 654
Size: 54 600
BssSI C'ACGA_G
Cuts at: 0 433 654
Size: 433 221
BtgI C'CryG_G
Cuts at: 0 22 330 654
Size: 22 308 324
Fragments arranged by size:
324 308 22
EaeI y'GGCC_r
Cuts at: 0 127 204 654
Size: 127 77 450
Fragments arranged by size:
450 127 77
EagI C'GGCC_G
Cuts at: 0 127 204 654
Size: 127 77 450
Fragments arranged by size:
450 127 77
EarI CTCTTCn'nnn_
Cuts at: 0 223 654
Size: 223 431
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 163 654
Size: 163 491
HaeII r_GCGC'y
Cuts at: 0 288 469 654
Size: 288 181 185
Fragments arranged by size:
288 185 181
KasI G'GCGC_C
Cuts at: 0 284 465 654
Size: 284 181 189
Fragments arranged by size:
284 189 181
MslI CAynn'nnrTG
Cuts at: 0 295 654
Size: 295 359
MspA1I CmG'CkG
Cuts at: 0 24 198 654
Size: 24 174 456
Fragments arranged by size:
456 174 24
NaeI GCC'GGC
Cuts at: 0 145 464 654
Size: 145 319 190
Fragments arranged by size:
319 190 145
NarI GG'CG_CC
Cuts at: 0 285 466 654
Size: 285 181 188
Fragments arranged by size:
285 188 181
NgoMIV G'CCGG_C
Cuts at: 0 143 462 654
Size: 143 319 192
Fragments arranged by size:
319 192 143
PasI CC'CwG_GG
Cuts at: 0 599 654
Size: 599 55
PvuII CAG'CTG
Cuts at: 0 198 654
Size: 198 456
RsrII CG'GwC_CG
Cuts at: 0 153 654
Size: 153 501
SacII CC_GC'GG
Cuts at: 0 25 654
Size: 25 629
SapI GCTCTTCn'nnn_
Cuts at: 0 223 654
Size: 223 431
SfoI GGC'GCC
Cuts at: 0 286 467 654
Size: 286 181 187
Fragments arranged by size:
286 187 181
SgrAI Cr'CCGG_yG
Cuts at: 0 462 654
Size: 462 192
StuI AGG'CCT
Cuts at: 0 340 445 654
Size: 340 105 209
Fragments arranged by size:
340 209 105
StyI C'CwwG_G
Cuts at: 0 638 654
Size: 638 16
ZraI GAC'GTC
Cuts at: 0 171 654
Size: 171 483
Enzymes that do cut:
AatII AflIII AleI BanI BanII BbeI BbsI BciVI
Bme1580I BmgBI BsaI BsaHI BseRI BseYI BsgI BsiEI
Bsp1286I BsrBI BsrFI BssHII BssSI BtgI EaeI EagI
EarI EciI HaeII KasI MslI MspA1I NaeI NarI
NgoMIV PasI PvuII RsrII SacII SapI SfoI SgrAI
StuI StyI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbvCI BcgI
BclI BfrBI BglI BglII BlpI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaAI BsaBI BsaWI BsaXI BsiHKAI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI BsrGI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI SacI
SalI SanDI SbfI ScaI SexAI SfcI SfiI SgfI
SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI