(Linear) (Six-base) MAPSORT of: KIBB6910.seq  Check: 7602  from: 1  to: 672



KIBB6910.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:27  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     281     672 
   Size:        281     391
 
AflII C'TTAA_G

Cuts at:      0     316     672 
   Size:        316     356
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     416     672 
   Size:        416     256
 
ApoI r'AATT_y

Cuts at:      0      43      55     434     443     586     672 
   Size:         43      12     379       9     143      86

  Fragments arranged by size: 

                 379     143      86      43      12       9
 
BanII G_rGCy'C

Cuts at:      0     505     672 
   Size:        505     167
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     613     672 
   Size:        613      59
 
Bme1580I G_kGCm'C

Cuts at:      0     564     672 
   Size:        564     108
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     592     672 
   Size:        592      80
 
BsmI GAATG_Cn'

Cuts at:      0     375     672 
   Size:        375     297
 
Bsp1286I G_dGCh'C

Cuts at:      0     505     564     672 
   Size:        505      59     108

  Fragments arranged by size: 

                 505     108      59
 
BstBI TT'CG_AA

Cuts at:      0     438     672 
   Size:        438     234
 
BstYI r'GATC_y

Cuts at:      0     312     672 
   Size:        312     360
 
BtgI C'CryG_G

Cuts at:      0     659     672 
   Size:        659      13
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     281     672 
   Size:        281     391
 
EcoO109I rG'GnC_Cy

Cuts at:      0     576     672 
   Size:        576      96
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     258     439     672 
   Size:        258     181     233

  Fragments arranged by size: 

                 258     233     181
 
HindIII A'AGCT_T

Cuts at:      0     647     672 
   Size:        647      25
 
MslI CAynn'nnrTG

Cuts at:      0     527     672 
   Size:        527     145
 
PfoI T'CCnGG_A

Cuts at:      0     308     672 
   Size:        308     364
 
PsiI TTA'TAA

Cuts at:      0     668     672 
   Size:        668       4
 
PstI C_TGCA'G

Cuts at:      0     395     405     672 
   Size:        395      10     267

  Fragments arranged by size: 

                 395     267      10
 
ScaI AGT'ACT

Cuts at:      0     293     672 
   Size:        293     379
 
SfcI C'TryA_G

Cuts at:      0     391     401     672 
   Size:        391      10     271

  Fragments arranged by size: 

                 391     271      10
 
SmlI C'TyrA_G

Cuts at:      0     316     571     672 
   Size:        316     255     101

  Fragments arranged by size: 

                 316     255     101
 
SspI AAT'ATT

Cuts at:      0     328     480     672 
   Size:        328     152     192

  Fragments arranged by size: 

                 328     192     152
 
TatI w'GTAC_w

Cuts at:      0     291     672 
   Size:        291     381
 
XmnI GAAnn'nnTTC

Cuts at:      0      55     672 
   Size:         55     617
 

 Enzymes that do cut:

     AcuI    AflII     AlfI     ApoI    BanII     BbsI Bme1580I    BpuEI
     BsmI Bsp1286I    BstBI    BstYI     BtgI  Eco57MI EcoO109I     FalI
  HindIII     MslI     PfoI     PsiI     PstI     ScaI     SfcI     SmlI
     SspI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI
     FspI    FspAI    HaeII    Hin4I   HincII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI
   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     StyI
     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI