(Linear) (Six-base) MAPSORT of: KIBB6905.seq  Check: 9491  from: 1  to: 612



KIBB6905.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AflII C'TTAA_G

Cuts at:      0     445     612 
   Size:        445     167
 
ApoI r'AATT_y

Cuts at:      0     529     612 
   Size:        529      83
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     195     612 
   Size:        195     417
 
BglII A'GATC_T

Cuts at:      0     387     612 
   Size:        387     225
 
BsaBI GATnn'nnATC

Cuts at:      0     128     392     612 
   Size:        128     264     220

  Fragments arranged by size: 

                 264     220     128
 
BsiEI CG_ry'CG

Cuts at:      0      11     612 
   Size:         11     601
 
BsrFI r'CCGG_y

Cuts at:      0      10      44     612 
   Size:         10      34     568

  Fragments arranged by size: 

                 568      34      10
 
BssSI C'ACGA_G

Cuts at:      0      49     612 
   Size:         49     563
 
BstYI r'GATC_y

Cuts at:      0     387     612 
   Size:        387     225
 
BtgI C'CryG_G

Cuts at:      0       5     612 
   Size:          5     607
 
BtsI GCAGTG_nn'

Cuts at:      0     166     612 
   Size:        166     446
 
EaeI y'GGCC_r

Cuts at:      0       2       8      30     612 
   Size:          2       6      22     582

  Fragments arranged by size: 

                 582      22       6       2
 
EagI C'GGCC_G

Cuts at:      0       8     612 
   Size:          8     604
 
EarI CTCTTCn'nnn_

Cuts at:      0     212     612 
   Size:        212     400
 
HaeII r_GCGC'y

Cuts at:      0      88     612 
   Size:         88     524
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     317     349     612 
   Size:        317      32     263

  Fragments arranged by size: 

                 317     263      32
 
HindIII A'AGCT_T

Cuts at:      0     497     612 
   Size:        497     115
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     157     612 
   Size:        157     455
 
MspA1I CmG'CkG

Cuts at:      0       7     612 
   Size:          7     605
 
NaeI GCC'GGC

Cuts at:      0      12     612 
   Size:         12     600
 
NgoMIV G'CCGG_C

Cuts at:      0      10     612 
   Size:         10     602
 
SacII CC_GC'GG

Cuts at:      0       8     612 
   Size:          8     604
 
SmlI C'TyrA_G

Cuts at:      0     445     612 
   Size:        445     167
 

 Enzymes that do cut:

    AflII     ApoI    BciVI    BglII    BsaBI    BsiEI    BsrFI    BssSI
    BstYI     BtgI     BtsI     EaeI     EagI     EarI    HaeII    Hin4I
  HindIII     MmeI   MspA1I     NaeI   NgoMIV    SacII     SmlI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrGI   BssHII   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI   HincII     HpaI     KasI     KpnI
     MfeI     MluI     MscI     MslI     NarI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI