(Linear) (Six-base) MAPSORT of: KIBB6889.seq  Check: 6379  from: 1  to: 549



KIBB6889.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AatII G_ACGT'C

Cuts at:      0     436     549 
   Size:        436     113
 
AccI GT'mk_AC

Cuts at:      0     242     458     549 
   Size:        242     216      91

  Fragments arranged by size: 

                 242     216      91
 
AvaI C'yCGr_G

Cuts at:      0      30     549 
   Size:         30     519
 
BanI G'GyrC_C

Cuts at:      0     178     549 
   Size:        178     371
 
BanII G_rGCy'C

Cuts at:      0      31      79     549 
   Size:         31      48     470

  Fragments arranged by size: 

                 470      48      31
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     116     549 
   Size:        116     433
 
BbvCI CC'TCA_GC

Cuts at:      0     404     549 
   Size:        404     145
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     451     485     549 
   Size:        451      34      64

  Fragments arranged by size: 

                 451      64      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     162     549 
   Size:        162     387
 
Bme1580I G_kGCm'C

Cuts at:      0     181     329     549 
   Size:        181     148     220

  Fragments arranged by size: 

                 220     181     148
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     156     549 
   Size:        156     393
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     178     379     549 
   Size:        178     201     170

  Fragments arranged by size: 

                 201     178     170
 
Bpu10I CC'TnA_GC

Cuts at:      0     404     549 
   Size:        404     145
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     381     549 
   Size:        381     168
 
BsaHI Gr'CG_yC

Cuts at:      0     433     549 
   Size:        433     116
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     421     549 
   Size:        421     128
 
BseYI C'CCAG_C

Cuts at:      0      24     549 
   Size:         24     525
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      82     549 
   Size:         82     467
 
BsiWI C'GTAC_G

Cuts at:      0     380     549 
   Size:        380     169
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     424     549 
   Size:        424     125
 
Bsp1286I G_dGCh'C

Cuts at:      0      31      79     181     329     549 
   Size:         31      48     102     148     220

  Fragments arranged by size: 

                 220     148     102      48      31
 
BsrGI T'GTAC_A

Cuts at:      0     209     549 
   Size:        209     340
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     336     549 
   Size:        336     213
 
BstYI r'GATC_y

Cuts at:      0     161     549 
   Size:        161     388
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     530     549 
   Size:        530      19
 
EaeI y'GGCC_r

Cuts at:      0     222     437     549 
   Size:        222     215     112

  Fragments arranged by size: 

                 222     215     112
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     178     379     549 
   Size:        178     201     170

  Fragments arranged by size: 

                 201     178     170
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     403     549 
   Size:        403     146
 
EcoO109I rG'GnC_Cy

Cuts at:      0     204     396     549 
   Size:        204     192     153

  Fragments arranged by size: 

                 204     192     153
 
HincII GTy'rAC

Cuts at:      0     255     549 
   Size:        255     294
 
MslI CAynn'nnrTG

Cuts at:      0     304     549 
   Size:        304     245
 
PfoI T'CCnGG_A

Cuts at:      0     164     269     549 
   Size:        164     105     280

  Fragments arranged by size: 

                 280     164     105
 
PpuMI rG'GwC_Cy

Cuts at:      0     204     549 
   Size:        204     345
 
SfcI C'TryA_G

Cuts at:      0     243     549 
   Size:        243     306
 
TatI w'GTAC_w

Cuts at:      0       8     209     549 
   Size:          8     201     340

  Fragments arranged by size: 

                 340     201       8
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     336     549 
   Size:        336     213
 
ZraI GAC'GTC

Cuts at:      0     434     549 
   Size:        434     115
 

 Enzymes that do cut:

    AatII     AccI     AvaI     BanI    BanII     BbsI    BbvCI     BcgI
    BciVI Bme1580I     BplI     BpmI   Bpu10I     BsaI    BsaHI    BseRI
    BseYI     BsgI    BsiWI    BsmBI Bsp1286I    BsrGI    BstXI    BstYI
    BtgZI     EaeI  Eco57MI    EcoNI EcoO109I   HincII     MslI     PfoI
    PpuMI     SfcI     TatI     XcmI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI     BclI
    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI    BpuEI
    BsaAI    BsaBI    BsaWI    BsaXI    BsiEI  BsiHKAI     BsmI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI
    BstBI   BstEII  BstZ17I   Bsu36I     BtgI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI