(Linear) (Six-base) MAPSORT of: KIBB6888.seq  Check: 4099  from: 1  to: 603



KIBB6888.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AgeI A'CCGG_T

Cuts at:      0      81     603 
   Size:         81     522
 
BanI G'GyrC_C

Cuts at:      0      84     603 
   Size:         84     519
 
Bme1580I G_kGCm'C

Cuts at:      0      89     603 
   Size:         89     514
 
BmgBI CAC'GTC

Cuts at:      0     399     603 
   Size:        399     204
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     426     475     603 
   Size:        426      49     128

  Fragments arranged by size: 

                 426     128      49
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     303     577     603 
   Size:        303     274      26

  Fragments arranged by size: 

                 303     274      26
 
Bpu10I CC'TnA_GC

Cuts at:      0      28     603 
   Size:         28     575
 
BsaAI yAC'GTr

Cuts at:      0     327     603 
   Size:        327     276
 
BsaWI w'CCGG_w

Cuts at:      0      81     110     603 
   Size:         81      29     493

  Fragments arranged by size: 

                 493      81      29
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     172     603 
   Size:        172     431
 
BsmI GAATG_Cn'

Cuts at:      0     500     603 
   Size:        500     103
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     442     603 
   Size:        442     161
 
Bsp1286I G_dGCh'C

Cuts at:      0      89     603 
   Size:         89     514
 
BsrFI r'CCGG_y

Cuts at:      0      81     603 
   Size:         81     522
 
BsrGI T'GTAC_A

Cuts at:      0     521     603 
   Size:        521      82
 
BssSI C'ACGA_G

Cuts at:      0     568     603 
   Size:        568      35
 
BstBI TT'CG_AA

Cuts at:      0     150     603 
   Size:        150     453
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     327     603 
   Size:        327     276
 
BstYI r'GATC_y

Cuts at:      0      56     182     603 
   Size:         56     126     421

  Fragments arranged by size: 

                 421     126      56
 
Bsu36I CC'TnA_GG

Cuts at:      0     364     603 
   Size:        364     239
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     303     577     603 
   Size:        303     274      26

  Fragments arranged by size: 

                 303     274      26
 
FspI TGC'GCA

Cuts at:      0     499     603 
   Size:        499     104
 
HaeII r_GCGC'y

Cuts at:      0       7     603 
   Size:          7     596
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     583     603 
   Size:        583      20
 
MspA1I CmG'CkG

Cuts at:      0     300     603 
   Size:        300     303
 
NspI r_CATG'y

Cuts at:      0     387     603 
   Size:        387     216
 
StyI C'CwwG_G

Cuts at:      0     455     603 
   Size:        455     148
 
TatI w'GTAC_w

Cuts at:      0     161     521     603 
   Size:        161     360      82

  Fragments arranged by size: 

                 360     161      82
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     102     304     462     603 
   Size:        102     202     158     141

  Fragments arranged by size: 

                 202     158     141     102
 

 Enzymes that do cut:

     AgeI     BanI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BsaAI
    BsaWI    BseRI     BsmI    BsmBI Bsp1286I    BsrFI    BsrGI    BssSI
    BstBI    BstXI    BstYI   Bsu36I  Eco57MI     FspI    HaeII    Hin4I
   MspA1I     NspI     StyI     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI     BmtI     BplI    BpuEI     BsaI    BsaBI
    BsaHI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI    BspEI
    BspHI    BspMI    BsrBI    BsrDI   BssHII   BstAPI   BstEII  BstZ17I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI