(Linear) (Six-base) MAPSORT of: KIBB6887.seq Check: 4960 from: 1 to: 549 KIBB6887.orf With 182 enzymes: SgfI * June 26, 2008 10:48 .. AlwNI CAG_nnn'CTG Cuts at: 0 274 536 549 Size: 274 262 13 Fragments arranged by size: 274 262 13 ApaLI G'TGCA_C Cuts at: 0 309 549 Size: 309 240 BanII G_rGCy'C Cuts at: 0 266 440 549 Size: 266 174 109 Fragments arranged by size: 266 174 109 BglII A'GATC_T Cuts at: 0 404 549 Size: 404 145 Bme1580I G_kGCm'C Cuts at: 0 313 429 549 Size: 313 116 120 Fragments arranged by size: 313 120 116 BmgBI CAC'GTC Cuts at: 0 96 549 Size: 96 453 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 469 549 Size: 469 80 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 280 549 Size: 280 269 Bpu10I CC'TnA_GC Cuts at: 0 50 549 Size: 50 499 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 454 484 549 Size: 454 30 65 Fragments arranged by size: 454 65 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 152 430 448 549 Size: 152 278 18 101 Fragments arranged by size: 278 152 101 18 BseYI C'CCAG_C Cuts at: 0 197 225 549 Size: 197 28 324 Fragments arranged by size: 324 197 28 BsiHKAI G_wGCw'C Cuts at: 0 313 440 549 Size: 313 127 109 Fragments arranged by size: 313 127 109 Bsp1286I G_dGCh'C Cuts at: 0 266 313 429 440 549 Size: 266 47 116 11 109 Fragments arranged by size: 266 116 109 47 11 BstYI r'GATC_y Cuts at: 0 404 549 Size: 404 145 BtgI C'CryG_G Cuts at: 0 512 549 Size: 512 37 EaeI y'GGCC_r Cuts at: 0 488 549 Size: 488 61 EarI CTCTTCn'nnn_ Cuts at: 0 22 549 Size: 22 527 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 367 549 Size: 367 182 EcoICRI GAG'CTC Cuts at: 0 438 549 Size: 438 111 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 280 549 Size: 280 269 EcoNI CCTnn'n_nnAGG Cuts at: 0 528 549 Size: 528 21 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 454 486 549 Size: 454 32 63 Fragments arranged by size: 454 63 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 234 549 Size: 234 315 MscI TGG'CCA Cuts at: 0 490 549 Size: 490 59 MspA1I CmG'CkG Cuts at: 0 75 497 549 Size: 75 422 52 Fragments arranged by size: 422 75 52 PflMI CCAn_nnn'nTGG Cuts at: 0 513 549 Size: 513 36 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 166 198 549 Size: 166 32 351 Fragments arranged by size: 351 166 32 PshAI GACnn'nnGTC Cuts at: 0 455 549 Size: 455 94 PstI C_TGCA'G Cuts at: 0 39 549 Size: 39 510 SacI G_AGCT'C Cuts at: 0 440 549 Size: 440 109 SbfI CC_TGCA'GG Cuts at: 0 39 549 Size: 39 510 SfcI C'TryA_G Cuts at: 0 35 111 369 549 Size: 35 76 258 180 Fragments arranged by size: 258 180 76 35 StuI AGG'CCT Cuts at: 0 336 549 Size: 336 213 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 121 549 Size: 121 428 TatI w'GTAC_w Cuts at: 0 101 549 Size: 101 448 Tth111I GACn'n_nGTC Cuts at: 0 94 549 Size: 94 455 Enzymes that do cut: AlwNI ApaLI BanII BglII Bme1580I BmgBI BplI BpmI Bpu10I BsaXI BseRI BseYI BsiHKAI Bsp1286I BstYI BtgI EaeI EarI EciI EcoICRI Eco57MI EcoNI Hin4I MmeI MscI MspA1I PflMI PpiI PshAI PstI SacI SbfI SfcI StuI TaqII TatI Tth111I Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BlpI BmrI BmtI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PfoI PmeI PmlI PpuMI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI SapI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StyI SwaI TstI XbaI XcmI XhoI XmaI XmnI ZraI