(Linear) (Six-base) MAPSORT of: KIBB6877.seq  Check: 7395  from: 1  to: 540



KIBB6877.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     322     540 
   Size:        322     218
 
AccI GT'mk_AC

Cuts at:      0      68     540 
   Size:         68     472
 
Acc65I G'GTAC_C

Cuts at:      0     200     540 
   Size:        200     340
 
AgeI A'CCGG_T

Cuts at:      0     509     540 
   Size:        509      31
 
AlwNI CAG_nnn'CTG

Cuts at:      0     314     540 
   Size:        314     226
 
AvaI C'yCGr_G

Cuts at:      0      18     540 
   Size:         18     522
 
BamHI G'GATC_C

Cuts at:      0     408     540 
   Size:        408     132
 
BanI G'GyrC_C

Cuts at:      0     200     540 
   Size:        200     340
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     466     540 
   Size:        466      74
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      84     364     540 
   Size:         84     280     176

  Fragments arranged by size: 

                 280     176      84
 
BsaWI w'CCGG_w

Cuts at:      0     509     540 
   Size:        509      31
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      40      70      82     112     540 
   Size:         40      30      12      30     428

  Fragments arranged by size: 

                 428      40      30      30      12
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     331     540 
   Size:        331     209
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     322     458     540 
   Size:        322     136      82

  Fragments arranged by size: 

                 322     136      82
 
BsrDI GCAATG_nn'

Cuts at:      0     477     540 
   Size:        477      63
 
BsrFI r'CCGG_y

Cuts at:      0     509     540 
   Size:        509      31
 
BssSI C'ACGA_G

Cuts at:      0     282     403     540 
   Size:        282     121     137

  Fragments arranged by size: 

                 282     137     121
 
BstEII G'GTnAC_C

Cuts at:      0     361     540 
   Size:        361     179
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     240     540 
   Size:        240     300
 
BstYI r'GATC_y

Cuts at:      0     408     540 
   Size:        408     132
 
EaeI y'GGCC_r

Cuts at:      0     437     540 
   Size:        437     103
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      84     364     540 
   Size:         84     280     176

  Fragments arranged by size: 

                 280     176      84
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     355     540 
   Size:        355     185
 
EcoO109I rG'GnC_Cy

Cuts at:      0      27     375     495     517     540 
   Size:         27     348     120      22      23

  Fragments arranged by size: 

                 348     120      27      23      22
 
HincII GTy'rAC

Cuts at:      0      69     540 
   Size:         69     471
 
KpnI G_GTAC'C

Cuts at:      0     204     540 
   Size:        204     336
 
MscI TGG'CCA

Cuts at:      0     439     540 
   Size:        439     101
 
PasI CC'CwG_GG

Cuts at:      0     357     540 
   Size:        357     183
 
PfoI T'CCnGG_A

Cuts at:      0     143     540 
   Size:        143     397
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     286     318     540 
   Size:        286      32     222

  Fragments arranged by size: 

                 286     222      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      27     375     495     517     540 
   Size:         27     348     120      22      23

  Fragments arranged by size: 

                 348     120      27      23      22
 
PstI C_TGCA'G

Cuts at:      0     432     540 
   Size:        432     108
 
SalI G'TCGA_C

Cuts at:      0      67     540 
   Size:         67     473
 
SanDI GG'GwC_CC

Cuts at:      0     517     540 
   Size:        517      23
 
SexAI A'CCwGG_T

Cuts at:      0     371     540 
   Size:        371     169
 
SfcI C'TryA_G

Cuts at:      0      81     428     540 
   Size:         81     347     112

  Fragments arranged by size: 

                 347     112      81
 
SmaI CCC'GGG

Cuts at:      0      20     540 
   Size:         20     520
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      82     114     540 
   Size:         82      32     426

  Fragments arranged by size: 

                 426      82      32
 
XmaI C'CCGG_G

Cuts at:      0      18     540 
   Size:         18     522
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AgeI    AlwNI     AvaI    BamHI     BanI
     BbsI     BpmI    BsaWI    BsaXI     BsgI    BspMI    BsrDI    BsrFI
    BssSI   BstEII    BstXI    BstYI     EaeI  Eco57MI    EcoNI EcoO109I
   HincII     KpnI     MscI     PasI     PfoI     PpiI    PpuMI     PstI
     SalI    SanDI    SexAI     SfcI     SmaI     TstI     XmaI

 Enzymes that do not cut: 

    AatII     AclI     AcuI     AfeI    AflII   AflIII     AhdI     AleI
     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BanII     BbeI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BseRI
    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BsrBI    BsrGI   BssHII   BstAPI    BstBI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I  HindIII     HpaI     KasI     MfeI
     MluI     MmeI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PciI
    PflMI     PmeI     PmlI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SapI     SbfI     ScaI
     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI  Tth111I
     XbaI     XcmI     XhoI     XmnI     ZraI