Restriction map for
pF1KB6876
(Linear) (Six-base) MAPSORT of: KIBB6876.seq Check: 3802 from: 1 to: 600
KIBB6876.orf
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
Acc65I G'GTAC_C
Cuts at: 0 92 600
Size: 92 508
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 600
Size: 127 473
AlwNI CAG_nnn'CTG
Cuts at: 0 303 600
Size: 303 297
ApaI G_GGCC'C
Cuts at: 0 24 600
Size: 24 576
ApoI r'AATT_y
Cuts at: 0 511 542 600
Size: 511 31 58
Fragments arranged by size:
511 58 31
AvaI C'yCGr_G
Cuts at: 0 403 600
Size: 403 197
BanI G'GyrC_C
Cuts at: 0 92 235 600
Size: 92 143 365
Fragments arranged by size:
365 143 92
BanII G_rGCy'C
Cuts at: 0 24 600
Size: 24 576
BclI T'GATC_A
Cuts at: 0 395 600
Size: 395 205
BglII A'GATC_T
Cuts at: 0 461 600
Size: 461 139
Bme1580I G_kGCm'C
Cuts at: 0 24 600
Size: 24 576
BmgBI CAC'GTC
Cuts at: 0 330 411 600
Size: 330 81 189
Fragments arranged by size:
330 189 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 102 600
Size: 102 498
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 325 388 600
Size: 61 264 63 212
Fragments arranged by size:
264 212 63 61
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 67 97 600
Size: 67 30 503
Fragments arranged by size:
503 67 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 58 88 91 600
Size: 58 30 3 509
Fragments arranged by size:
509 58 30 3
BseYI C'CCAG_C
Cuts at: 0 134 197 600
Size: 134 63 403
Fragments arranged by size:
403 134 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 600
Size: 253 347
BsiEI CG_ry'CG
Cuts at: 0 126 600
Size: 126 474
BsiHKAI G_wGCw'C
Cuts at: 0 88 600
Size: 88 512
Bsp1286I G_dGCh'C
Cuts at: 0 24 88 600
Size: 24 64 512
Fragments arranged by size:
512 64 24
BsrFI r'CCGG_y
Cuts at: 0 121 600
Size: 121 479
BstYI r'GATC_y
Cuts at: 0 35 461 600
Size: 35 426 139
Fragments arranged by size:
426 139 35
BtgI C'CryG_G
Cuts at: 0 281 600
Size: 281 319
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 246 600
Size: 246 354
EaeI y'GGCC_r
Cuts at: 0 123 530 600
Size: 123 407 70
Fragments arranged by size:
407 123 70
EagI C'GGCC_G
Cuts at: 0 123 600
Size: 123 477
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 127 325 388 600
Size: 61 66 198 63 212
Fragments arranged by size:
212 198 66 63 61
EcoNI CCTnn'n_nnAGG
Cuts at: 0 28 600
Size: 28 572
EcoO109I rG'GnC_Cy
Cuts at: 0 20 21 600
Size: 20 1 579
Fragments arranged by size:
579 20 1
EcoRI G'AATT_C
Cuts at: 0 511 600
Size: 511 89
KpnI G_GTAC'C
Cuts at: 0 96 600
Size: 96 504
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 248 600
Size: 248 352
MscI TGG'CCA
Cuts at: 0 532 600
Size: 532 68
MspA1I CmG'CkG
Cuts at: 0 54 283 600
Size: 54 229 317
Fragments arranged by size:
317 229 54
NspI r_CATG'y
Cuts at: 0 205 600
Size: 205 395
PflMI CCAn_nnn'nTGG
Cuts at: 0 303 600
Size: 303 297
PfoI T'CCnGG_A
Cuts at: 0 38 600
Size: 38 562
PspOMI G'GGCC_C
Cuts at: 0 20 600
Size: 20 580
PstI C_TGCA'G
Cuts at: 0 53 600
Size: 53 547
PvuII CAG'CTG
Cuts at: 0 54 600
Size: 54 546
SacII CC_GC'GG
Cuts at: 0 284 600
Size: 284 316
ScaI AGT'ACT
Cuts at: 0 472 600
Size: 472 128
SfcI C'TryA_G
Cuts at: 0 49 600
Size: 49 551
StuI AGG'CCT
Cuts at: 0 190 600
Size: 190 410
StyI C'CwwG_G
Cuts at: 0 29 185 600
Size: 29 156 415
Fragments arranged by size:
415 156 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 148 600
Size: 148 452
TatI w'GTAC_w
Cuts at: 0 470 600
Size: 470 130
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 67 99 600
Size: 67 32 501
Fragments arranged by size:
501 67 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 36 300 600
Size: 36 264 300
Fragments arranged by size:
300 264 36
XmnI GAAnn'nnTTC
Cuts at: 0 511 600
Size: 511 89
Enzymes that do cut:
Acc65I AcuI AlwNI ApaI ApoI AvaI BanI BanII
BclI BglII Bme1580I BmgBI BplI BpmI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BsrFI BstYI BtgI
BtgZI EaeI EagI Eco57MI EcoNI EcoO109I EcoRI KpnI
MmeI MscI MspA1I NspI PflMI PfoI PspOMI PstI
PvuII SacII ScaI SfcI StuI StyI TaqII TatI
TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI
BfrBI BglI BlpI BmrI BmtI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtsI ClaI CspCI
DraI DraIII DrdI EarI EciI EcoICRI EcoRV FalI
FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI MfeI MluI MslI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SalI SanDI SapI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI SwaI Tth111I XbaI XhoI XmaI
ZraI