(Linear) (Six-base) MAPSORT of: KIBB6869.seq  Check: 4619  from: 1  to: 537



KIBB6869.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

Acc65I G'GTAC_C

Cuts at:      0     200     537 
   Size:        200     337
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     274     537 
   Size:        274     263
 
AgeI A'CCGG_T

Cuts at:      0     203     537 
   Size:        203     334
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     281     537 
   Size:        281     256
 
ApoI r'AATT_y

Cuts at:      0      83      96     537 
   Size:         83      13     441

  Fragments arranged by size: 

                 441      83      13
 
BanI G'GyrC_C

Cuts at:      0     200     537 
   Size:        200     337
 
BanII G_rGCy'C

Cuts at:      0     328     537 
   Size:        328     209
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     405     537 
   Size:        405     132
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     414     537 
   Size:        414     123
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     119     139     312     537 
   Size:        119      20     173     225

  Fragments arranged by size: 

                 225     173     119      20
 
BsaBI GATnn'nnATC

Cuts at:      0     506     537 
   Size:        506      31
 
BsaWI w'CCGG_w

Cuts at:      0     203     537 
   Size:        203     334
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     382     537 
   Size:        382     155
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     386     537 
   Size:        386     151
 
BsiHKAI G_wGCw'C

Cuts at:      0     328     374     537 
   Size:        328      46     163

  Fragments arranged by size: 

                 328     163      46
 
Bsp1286I G_dGCh'C

Cuts at:      0     328     374     537 
   Size:        328      46     163

  Fragments arranged by size: 

                 328     163      46
 
BsrFI r'CCGG_y

Cuts at:      0     203     537 
   Size:        203     334
 
Bsu36I CC'TnA_GG

Cuts at:      0      45     537 
   Size:         45     492
 
BtsI GCAGTG_nn'

Cuts at:      0      11     537 
   Size:         11     526
 
DraIII CAC_nnn'GTG

Cuts at:      0      23     537 
   Size:         23     514
 
EcoICRI GAG'CTC

Cuts at:      0     326     537 
   Size:        326     211
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     119     139     274     312     537 
   Size:        119      20     135      38     225

  Fragments arranged by size: 

                 225     135     119      38      20
 
EcoO109I rG'GnC_Cy

Cuts at:      0      41     139     537 
   Size:         41      98     398

  Fragments arranged by size: 

                 398      98      41
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     315     347     537 
   Size:        315      32     190

  Fragments arranged by size: 

                 315     190      32
 
HincII GTy'rAC

Cuts at:      0     334     537 
   Size:        334     203
 
KpnI G_GTAC'C

Cuts at:      0     204     537 
   Size:        204     333
 
MslI CAynn'nnrTG

Cuts at:      0     263     537 
   Size:        263     274
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      23     537 
   Size:         23     514
 
PfoI T'CCnGG_A

Cuts at:      0     135     537 
   Size:        135     402
 
PpuMI rG'GwC_Cy

Cuts at:      0      41     139     537 
   Size:         41      98     398

  Fragments arranged by size: 

                 398      98      41
 
SacI G_AGCT'C

Cuts at:      0     328     537 
   Size:        328     209
 

 Enzymes that do cut:

   Acc65I     AcuI     AgeI     AlfI     ApoI     BanI    BanII     BmrI
     BplI     BpmI    BsaBI    BsaWI    BseRI     BsgI  BsiHKAI Bsp1286I
    BsrFI   Bsu36I     BtsI   DraIII  EcoICRI  Eco57MI EcoO109I    Hin4I
   HincII     KpnI     MslI    PflMI     PfoI    PpuMI     SacI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AhdI
     AleI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmtI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaXI    BseYI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I     BtgI    BtgZI     ClaI    CspCI     DraI     DrdI
     EaeI     EagI     EarI     EciI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI     MfeI
     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI