Restriction map for
pF1KB6867
(Linear) (Six-base) MAPSORT of: KIBB6867.seq Check: 695 from: 1 to: 540
KIBB6867.orf
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AfeI AGC'GCT
Cuts at: 0 9 540
Size: 9 531
AleI CACnn'nnGTG
Cuts at: 0 137 540
Size: 137 403
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 27 540
Size: 27 513
AlwNI CAG_nnn'CTG
Cuts at: 0 207 540
Size: 207 333
ApaI G_GGCC'C
Cuts at: 0 361 385 470 540
Size: 361 24 85 70
Fragments arranged by size:
361 85 70 24
BanI G'GyrC_C
Cuts at: 0 504 540
Size: 504 36
BanII G_rGCy'C
Cuts at: 0 361 385 470 540
Size: 361 24 85 70
Fragments arranged by size:
361 85 70 24
BglI GCCn_nnn'nGGC
Cuts at: 0 513 540
Size: 513 27
Bme1580I G_kGCm'C
Cuts at: 0 361 385 470 509 540
Size: 361 24 85 39 31
Fragments arranged by size:
361 85 39 31 24
BsaI GGTCTCn'nnnn_
Cuts at: 0 474 540
Size: 474 66
BsaBI GATnn'nnATC
Cuts at: 0 23 540
Size: 23 517
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 260 263 311 377 380 540
Size: 260 3 48 66 3 160
Fragments arranged by size:
260 160 66 48 3 3
BseYI C'CCAG_C
Cuts at: 0 304 540
Size: 304 236
Bsp1286I G_dGCh'C
Cuts at: 0 361 385 470 509 540
Size: 361 24 85 39 31
Fragments arranged by size:
361 85 39 31 24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 423 540
Size: 423 117
BtgI C'CryG_G
Cuts at: 0 14 437 540
Size: 14 423 103
Fragments arranged by size:
423 103 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 301 540
Size: 301 239
BtsI GCAGTG_nn'
Cuts at: 0 132 144 540
Size: 132 12 396
Fragments arranged by size:
396 132 12
EarI CTCTTCn'nnn_
Cuts at: 0 55 185 540
Size: 55 130 355
Fragments arranged by size:
355 130 55
EcoNI CCTnn'n_nnAGG
Cuts at: 0 278 540
Size: 278 262
EcoO109I rG'GnC_Cy
Cuts at: 0 316 357 358 382 412 467 540
Size: 316 41 1 24 30 55 73
Fragments arranged by size:
316 73 55 41 30 24 1
HaeII r_GCGC'y
Cuts at: 0 11 540
Size: 11 529
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 100 132 282 314 369 401 468 500
Size: 100 32 150 32 55 32 67 32
Cuts at: 500 540
Size: 40
Fragments arranged by size:
150 100 67 55 40 32 32 32
32
MslI CAynn'nnrTG
Cuts at: 0 29 137 502 540
Size: 29 108 365 38
Fragments arranged by size:
365 108 38 29
NcoI C'CATG_G
Cuts at: 0 437 540
Size: 437 103
NdeI CA'TA_TG
Cuts at: 0 350 449 540
Size: 350 99 91
Fragments arranged by size:
350 99 91
PfoI T'CCnGG_A
Cuts at: 0 420 540
Size: 420 120
PpuMI rG'GwC_Cy
Cuts at: 0 316 412 540
Size: 316 96 128
Fragments arranged by size:
316 128 96
PspOMI G'GGCC_C
Cuts at: 0 357 381 466 540
Size: 357 24 85 74
Fragments arranged by size:
357 85 74 24
PstI C_TGCA'G
Cuts at: 0 139 282 540
Size: 139 143 258
Fragments arranged by size:
258 143 139
SapI GCTCTTCn'nnn_
Cuts at: 0 185 540
Size: 185 355
SbfI CC_TGCA'GG
Cuts at: 0 282 540
Size: 282 258
SfcI C'TryA_G
Cuts at: 0 135 278 540
Size: 135 143 262
Fragments arranged by size:
262 143 135
StyI C'CwwG_G
Cuts at: 0 437 540
Size: 437 103
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 118 540
Size: 118 422
Enzymes that do cut:
AfeI AleI AloI AlwNI ApaI BanI BanII BglI
Bme1580I BsaI BsaBI BseRI BseYI Bsp1286I BspMI BtgI
BtgZI BtsI EarI EcoNI EcoO109I HaeII Hin4I MslI
NcoI NdeI PfoI PpuMI PspOMI PstI SapI SbfI
SfcI StyI TaqII
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AlfI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I ClaI CspCI DraI
DraIII DrdI EaeI EagI EciI EcoICRI Eco57MI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MspA1I NaeI
NarI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SanDI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI