Restriction map for
pF1KB6864
(Linear) (Six-base) MAPSORT of: KIBB6864.seq Check: 211 from: 1 to: 585
KIBB6864.orf
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AflII C'TTAA_G
Cuts at: 0 283 585
Size: 283 302
AlwNI CAG_nnn'CTG
Cuts at: 0 189 585
Size: 189 396
ApoI r'AATT_y
Cuts at: 0 350 541 571 585
Size: 350 191 30 14
Fragments arranged by size:
350 191 30 14
AvaI C'yCGr_G
Cuts at: 0 63 557 585
Size: 63 494 28
Fragments arranged by size:
494 63 28
BanI G'GyrC_C
Cuts at: 0 3 73 585
Size: 3 70 512
Fragments arranged by size:
512 70 3
BbeI G_GCGC'C
Cuts at: 0 7 585
Size: 7 578
BciVI GTATCCnnnnn_n'
Cuts at: 0 493 585
Size: 493 92
Bme1580I G_kGCm'C
Cuts at: 0 523 585
Size: 523 62
BmrI ACTGGGnnnn_n'
Cuts at: 0 177 585
Size: 177 408
BsaI GGTCTCn'nnnn_
Cuts at: 0 228 585
Size: 228 357
BsaHI Gr'CG_yC
Cuts at: 0 4 585
Size: 4 581
BsaWI w'CCGG_w
Cuts at: 0 545 585
Size: 545 40
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 194 224 446 476 585
Size: 194 30 222 30 109
Fragments arranged by size:
222 194 109 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 67 585
Size: 67 518
BseYI C'CCAG_C
Cuts at: 0 450 585
Size: 450 135
Bsp1286I G_dGCh'C
Cuts at: 0 523 585
Size: 523 62
BspEI T'CCGG_A
Cuts at: 0 545 585
Size: 545 40
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 434 585
Size: 434 151
BsrFI r'CCGG_y
Cuts at: 0 112 585
Size: 112 473
BssHII G'CGCG_C
Cuts at: 0 143 585
Size: 143 442
Bsu36I CC'TnA_GG
Cuts at: 0 402 585
Size: 402 183
BtgI C'CryG_G
Cuts at: 0 87 585
Size: 87 498
BtsI GCAGTG_nn'
Cuts at: 0 523 585
Size: 523 62
EaeI y'GGCC_r
Cuts at: 0 386 585
Size: 386 199
EarI CTCTTCn'nnn_
Cuts at: 0 121 585
Size: 121 464
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 355 585
Size: 355 230
EcoRI G'AATT_C
Cuts at: 0 541 571 585
Size: 541 30 14
Fragments arranged by size:
541 30 14
HaeII r_GCGC'y
Cuts at: 0 7 585
Size: 7 578
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 8 40 290 322 400 432 585
Size: 8 32 250 32 78 32 153
Fragments arranged by size:
250 153 78 32 32 32 8
KasI G'GCGC_C
Cuts at: 0 3 585
Size: 3 582
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 443 585
Size: 443 142
MscI TGG'CCA
Cuts at: 0 388 585
Size: 388 197
MspA1I CmG'CkG
Cuts at: 0 71 503 585
Size: 71 432 82
Fragments arranged by size:
432 82 71
NaeI GCC'GGC
Cuts at: 0 114 585
Size: 114 471
NarI GG'CG_CC
Cuts at: 0 4 585
Size: 4 581
NgoMIV G'CCGG_C
Cuts at: 0 112 585
Size: 112 473
PflMI CCAn_nnn'nTGG
Cuts at: 0 503 585
Size: 503 82
PsiI TTA'TAA
Cuts at: 0 356 585
Size: 356 229
PvuII CAG'CTG
Cuts at: 0 503 585
Size: 503 82
SapI GCTCTTCn'nnn_
Cuts at: 0 121 585
Size: 121 464
SfcI C'TryA_G
Cuts at: 0 161 585
Size: 161 424
SfoI GGC'GCC
Cuts at: 0 5 585
Size: 5 580
SmaI CCC'GGG
Cuts at: 0 65 585
Size: 65 520
SmlI C'TyrA_G
Cuts at: 0 283 585
Size: 283 302
SrfI GCCC'GGGC
Cuts at: 0 65 585
Size: 65 520
TatI w'GTAC_w
Cuts at: 0 252 561 585
Size: 252 309 24
Fragments arranged by size:
309 252 24
Tth111I GACn'n_nGTC
Cuts at: 0 379 585
Size: 379 206
XmaI C'CCGG_G
Cuts at: 0 63 585
Size: 63 522
Enzymes that do cut:
AflII AlwNI ApoI AvaI BanI BbeI BciVI Bme1580I
BmrI BsaI BsaHI BsaWI BsaXI BseRI BseYI Bsp1286I
BspEI BspMI BsrFI BssHII Bsu36I BtgI BtsI EaeI
EarI EciI EcoRI HaeII Hin4I KasI MmeI MscI
MspA1I NaeI NarI NgoMIV PflMI PsiI PvuII SapI
SfcI SfoI SmaI SmlI SrfI TatI Tth111I XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BanII BbsI BbvCI
BcgI BclI BfrBI BglI BglII BlpI BmgBI BmtI
BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspHI BsrBI BsrDI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EcoICRI Eco57MI
EcoNI EcoO109I EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MslI NcoI NdeI
NheI NotI NruI NsiI NspI PacI PasI PciI
PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI
SphI SspI StuI StyI SwaI TaqII TstI XbaI
XcmI XhoI XmnI ZraI