(Linear) (Six-base) MAPSORT of: KIBB6861.seq  Check: 1689  from: 1  to: 537



KIBB6861.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

Acc65I G'GTAC_C

Cuts at:      0     215     537 
   Size:        215     322
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     211     473     537 
   Size:        211     262      64

  Fragments arranged by size: 

                 262     211      64
 
AleI CACnn'nnGTG

Cuts at:      0     498     537 
   Size:        498      39
 
AlwNI CAG_nnn'CTG

Cuts at:      0     189     537 
   Size:        189     348
 
ApoI r'AATT_y

Cuts at:      0     208     537 
   Size:        208     329
 
AvaI C'yCGr_G

Cuts at:      0      79     521     537 
   Size:         79     442      16

  Fragments arranged by size: 

                 442      79      16
 
BanI G'GyrC_C

Cuts at:      0     215     262     537 
   Size:        215      47     275

  Fragments arranged by size: 

                 275     215      47
 
BanII G_rGCy'C

Cuts at:      0     138     537 
   Size:        138     399
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     353     537 
   Size:        353     184
 
Bme1580I G_kGCm'C

Cuts at:      0     265     537 
   Size:        265     272
 
BsaHI Gr'CG_yC

Cuts at:      0      13     537 
   Size:         13     524
 
BseYI C'CCAG_C

Cuts at:      0     182     395     537 
   Size:        182     213     142

  Fragments arranged by size: 

                 213     182     142
 
Bsp1286I G_dGCh'C

Cuts at:      0     138     265     537 
   Size:        138     127     272

  Fragments arranged by size: 

                 272     138     127
 
BsrBI CCG'CTC

Cuts at:      0     528     537 
   Size:        528       9
 
BsrDI GCAATG_nn'

Cuts at:      0     344     537 
   Size:        344     193
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     500     537 
   Size:        500      37
 
DraIII CAC_nnn'GTG

Cuts at:      0     439     537 
   Size:        439      98
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     211     473     537 
   Size:        211     262      64

  Fragments arranged by size: 

                 262     211      64
 
EcoO109I rG'GnC_Cy

Cuts at:      0      19     294     537 
   Size:         19     275     243

  Fragments arranged by size: 

                 275     243      19
 
EcoRI G'AATT_C

Cuts at:      0     208     537 
   Size:        208     329
 
HaeII r_GCGC'y

Cuts at:      0      70     537 
   Size:         70     467
 
HindIII A'AGCT_T

Cuts at:      0     455     537 
   Size:        455      82
 
KpnI G_GTAC'C

Cuts at:      0     219     537 
   Size:        219     318
 
MslI CAynn'nnrTG

Cuts at:      0     155     498     537 
   Size:        155     343      39

  Fragments arranged by size: 

                 343     155      39
 
MspA1I CmG'CkG

Cuts at:      0     186     417     537 
   Size:        186     231     120

  Fragments arranged by size: 

                 231     186     120
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     439     537 
   Size:        439      98
 
PpuMI rG'GwC_Cy

Cuts at:      0      19     294     537 
   Size:         19     275     243

  Fragments arranged by size: 

                 275     243      19
 
PsiI TTA'TAA

Cuts at:      0      59     537 
   Size:         59     478
 
PspXI vC'TCGA_Gb

Cuts at:      0      79     537 
   Size:         79     458
 
PstI C_TGCA'G

Cuts at:      0     422     537 
   Size:        422     115
 
PvuII CAG'CTG

Cuts at:      0     186     417     537 
   Size:        186     231     120

  Fragments arranged by size: 

                 231     186     120
 
SanDI GG'GwC_CC

Cuts at:      0      19     537 
   Size:         19     518
 
SexAI A'CCwGG_T

Cuts at:      0     435     537 
   Size:        435     102
 
SfcI C'TryA_G

Cuts at:      0     418     537 
   Size:        418     119
 
SmaI CCC'GGG

Cuts at:      0     523     537 
   Size:        523      14
 
SmlI C'TyrA_G

Cuts at:      0      79     537 
   Size:         79     458
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     196     537 
   Size:        196     341
 
XhoI C'TCGA_G

Cuts at:      0      79     537 
   Size:         79     458
 
XmaI C'CCGG_G

Cuts at:      0     521     537 
   Size:        521      16
 

 Enzymes that do cut:

   Acc65I     AcuI     AleI    AlwNI     ApoI     AvaI     BanI    BanII
    BciVI Bme1580I    BsaHI    BseYI Bsp1286I    BsrBI    BsrDI    BstXI
   DraIII  Eco57MI EcoO109I    EcoRI    HaeII  HindIII     KpnI     MslI
   MspA1I    PflMI    PpuMI     PsiI    PspXI     PstI    PvuII    SanDI
    SexAI     SfcI     SmaI     SmlI    TaqII     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI     BclI
    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI
    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
    EcoNI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I   HincII
     HpaI     KasI     MfeI     MluI     MmeI     MscI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PshAI
   PspOMI     PsrI     PvuI    RsrII     SacI    SacII     SalI     SapI
     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI     TatI     TstI
  Tth111I     XbaI     XcmI     XmnI     ZraI