(Linear) (Six-base) MAPSORT of: KIBB6853.seq  Check: 2682  from: 1  to: 531



KIBB6853.orf

 With 182 enzymes: SgfI * 

                        March 1, 2013 10:20  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     226     531 
   Size:        226     305
 
BamHI G'GATC_C

Cuts at:      0      81     531 
   Size:         81     450
 
BanII G_rGCy'C

Cuts at:      0     236     324     531 
   Size:        236      88     207

  Fragments arranged by size: 

                 236     207      88
 
BclI T'GATC_A

Cuts at:      0     519     531 
   Size:        519      12
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      70     531 
   Size:         70     461
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     190     531 
   Size:        190     341
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      29     308     483     531 
   Size:         29     279     175      48

  Fragments arranged by size: 

                 279     175      48      29
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     244     531 
   Size:        244     287
 
BsiEI CG_ry'CG

Cuts at:      0     444     531 
   Size:        444      87
 
BsiHKAI G_wGCw'C

Cuts at:      0     236     324     456     531 
   Size:        236      88     132      75

  Fragments arranged by size: 

                 236     132      88      75
 
Bsp1286I G_dGCh'C

Cuts at:      0     236     324     456     531 
   Size:        236      88     132      75

  Fragments arranged by size: 

                 236     132      88      75
 
BsrDI GCAATG_nn'

Cuts at:      0     259     531 
   Size:        259     272
 
BstYI r'GATC_y

Cuts at:      0      81     531 
   Size:         81     450
 
BtgI C'CryG_G

Cuts at:      0     134     531 
   Size:        134     397
 
EaeI y'GGCC_r

Cuts at:      0     161     531 
   Size:        161     370
 
EcoICRI GAG'CTC

Cuts at:      0     234     322     531 
   Size:        234      88     209

  Fragments arranged by size: 

                 234     209      88
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     190     226     531 
   Size:        190      36     305

  Fragments arranged by size: 

                 305     190      36
 
EcoO109I rG'GnC_Cy

Cuts at:      0      32     531 
   Size:         32     499
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     254     531 
   Size:        254     277
 
MscI TGG'CCA

Cuts at:      0     163     531 
   Size:        163     368
 
MspA1I CmG'CkG

Cuts at:      0     255     531 
   Size:        255     276
 
NcoI C'CATG_G

Cuts at:      0     134     531 
   Size:        134     397
 
PvuI CG_AT'CG

Cuts at:      0     444     531 
   Size:        444      87
 
PvuII CAG'CTG

Cuts at:      0     255     531 
   Size:        255     276
 
SacI G_AGCT'C

Cuts at:      0     236     324     531 
   Size:        236      88     207

  Fragments arranged by size: 

                 236     207      88
 
SfcI C'TryA_G

Cuts at:      0     313     531 
   Size:        313     218
 
SmlI C'TyrA_G

Cuts at:      0      44     323     462     531 
   Size:         44     279     139      69

  Fragments arranged by size: 

                 279     139      69      44
 
SspI AAT'ATT

Cuts at:      0      94     531 
   Size:         94     437
 
StyI C'CwwG_G

Cuts at:      0      27     134     531 
   Size:         27     107     397

  Fragments arranged by size: 

                 397     107      27
 
XmnI GAAnn'nnTTC

Cuts at:      0     383     531 
   Size:        383     148
 

 Enzymes that do cut:

     AcuI    BamHI    BanII     BclI     BmrI     BpmI    BpuEI    BseRI
    BsiEI  BsiHKAI Bsp1286I    BsrDI    BstYI     BtgI     EaeI  EcoICRI
  Eco57MI EcoO109I     MmeI     MscI   MspA1I     NcoI     PvuI    PvuII
     SacI     SfcI     SmlI     SspI     StyI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BanI
     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmtI     BplI   Bpu10I     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI
     EciI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MslI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI    RsrII    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     StuI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI