Restriction map for
pF1KB6844
(Linear) (Six-base) MAPSORT of: KIBB6844.seq Check: 3564 from: 1 to: 576
KIBB6844.orf
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AatII G_ACGT'C
Cuts at: 0 230 576
Size: 230 346
Acc65I G'GTAC_C
Cuts at: 0 290 576
Size: 290 286
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 514 576
Size: 514 62
AlwNI CAG_nnn'CTG
Cuts at: 0 426 576
Size: 426 150
ApaLI G'TGCA_C
Cuts at: 0 564 576
Size: 564 12
AvaI C'yCGr_G
Cuts at: 0 437 576
Size: 437 139
BanI G'GyrC_C
Cuts at: 0 34 290 305 340 402 576
Size: 34 256 15 35 62 174
Fragments arranged by size:
256 174 62 35 34 15
BanII G_rGCy'C
Cuts at: 0 259 576
Size: 259 317
BbeI G_GCGC'C
Cuts at: 0 38 576
Size: 38 538
BbsI GAAGACnn'nnnn_
Cuts at: 0 52 502 564 576
Size: 52 450 62 12
Fragments arranged by size:
450 62 52 12
BclI T'GATC_A
Cuts at: 0 59 576
Size: 59 517
BlpI GC'TnA_GC
Cuts at: 0 471 576
Size: 471 105
Bme1580I G_kGCm'C
Cuts at: 0 343 568 576
Size: 343 225 8
Fragments arranged by size:
343 225 8
BmrI ACTGGGnnnn_n'
Cuts at: 0 535 576
Size: 535 41
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 465 576
Size: 465 111
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 484 576
Size: 484 92
BsaHI Gr'CG_yC
Cuts at: 0 35 227 576
Size: 35 192 349
Fragments arranged by size:
349 192 35
BseYI C'CCAG_C
Cuts at: 0 482 576
Size: 482 94
BsiEI CG_ry'CG
Cuts at: 0 195 576
Size: 195 381
BsiHKAI G_wGCw'C
Cuts at: 0 472 530 568 576
Size: 472 58 38 8
Fragments arranged by size:
472 58 38 8
Bsp1286I G_dGCh'C
Cuts at: 0 259 343 472 530 568 576
Size: 259 84 129 58 38 8
Fragments arranged by size:
259 129 84 58 38 8
BsrBI CCG'CTC
Cuts at: 0 381 576
Size: 381 195
BstXI CCAn_nnnn'nTGG
Cuts at: 0 132 333 576
Size: 132 201 243
Fragments arranged by size:
243 201 132
BtgI C'CryG_G
Cuts at: 0 38 431 576
Size: 38 393 145
Fragments arranged by size:
393 145 38
BtsI GCAGTG_nn'
Cuts at: 0 309 576
Size: 309 267
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 90 125 576
Size: 90 35 451
Fragments arranged by size:
451 90 35
EaeI y'GGCC_r
Cuts at: 0 202 428 576
Size: 202 226 148
Fragments arranged by size:
226 202 148
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 484 514 576
Size: 484 30 62
Fragments arranged by size:
484 62 30
EcoO109I rG'GnC_Cy
Cuts at: 0 423 489 576
Size: 423 66 87
Fragments arranged by size:
423 87 66
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 69 576
Size: 69 507
HaeII r_GCGC'y
Cuts at: 0 38 576
Size: 38 538
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 80 103 112 135 576
Size: 80 23 9 23 441
Fragments arranged by size:
441 80 23 23 9
HincII GTy'rAC
Cuts at: 0 153 576
Size: 153 423
KasI G'GCGC_C
Cuts at: 0 34 576
Size: 34 542
KpnI G_GTAC'C
Cuts at: 0 294 576
Size: 294 282
MscI TGG'CCA
Cuts at: 0 430 576
Size: 430 146
MspA1I CmG'CkG
Cuts at: 0 175 486 576
Size: 175 311 90
Fragments arranged by size:
311 175 90
NarI GG'CG_CC
Cuts at: 0 35 576
Size: 35 541
NcoI C'CATG_G
Cuts at: 0 431 576
Size: 431 145
NspI r_CATG'y
Cuts at: 0 53 576
Size: 53 523
PvuI CG_AT'CG
Cuts at: 0 195 576
Size: 195 381
SfoI GGC'GCC
Cuts at: 0 36 576
Size: 36 540
SmaI CCC'GGG
Cuts at: 0 439 576
Size: 439 137
StyI C'CwwG_G
Cuts at: 0 431 576
Size: 431 145
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 420 576
Size: 420 156
TatI w'GTAC_w
Cuts at: 0 92 576
Size: 92 484
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 15 132 333 576
Size: 15 117 201 243
Fragments arranged by size:
243 201 117 15
XmaI C'CCGG_G
Cuts at: 0 437 576
Size: 437 139
ZraI GAC'GTC
Cuts at: 0 228 576
Size: 228 348
Enzymes that do cut:
AatII Acc65I AcuI AlwNI ApaLI AvaI BanI BanII
BbeI BbsI BclI BlpI Bme1580I BmrI BplI BpmI
BsaHI BseYI BsiEI BsiHKAI Bsp1286I BsrBI BstXI BtgI
BtsI CspCI EaeI Eco57MI EcoO109I FalI HaeII Hin4I
HincII KasI KpnI MscI MspA1I NarI NcoI NspI
PvuI SfoI SmaI StyI TaqII TatI XcmI XmaI
ZraI
Enzymes that do not cut:
AarI AccI AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BcgI BciVI BfrBI BglI
BglII BmgBI BmtI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaWI BsaXI BseRI BsgI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI DraI
DraIII DrdI EagI EarI EciI EcoICRI EcoNI EcoRI
EcoRV FseI FspI FspAI HindIII HpaI MfeI MluI
MmeI MslI NaeI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI Tth111I
XbaI XhoI XmnI