(Linear) (Six-base) MAPSORT of: KIBB6838.seq  Check: 6537  from: 1  to: 570



KIBB6838.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     146     570 
   Size:        146     424
 
AlwNI CAG_nnn'CTG

Cuts at:      0     264     546     570 
   Size:        264     282      24

  Fragments arranged by size: 

                 282     264      24
 
BanII G_rGCy'C

Cuts at:      0     413     570 
   Size:        413     157
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      61     570 
   Size:         61     509
 
BglII A'GATC_T

Cuts at:      0     149     570 
   Size:        149     421
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     110     570 
   Size:        110     460
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     170     570 
   Size:        170     400
 
BsaWI w'CCGG_w

Cuts at:      0     449     570 
   Size:        449     121
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      55     433     436     570 
   Size:         55     378       3     134

  Fragments arranged by size: 

                 378     134      55       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     559     570 
   Size:        559      11
 
BsiHKAI G_wGCw'C

Cuts at:      0     413     570 
   Size:        413     157
 
BsmI GAATG_Cn'

Cuts at:      0     480     570 
   Size:        480      90
 
Bsp1286I G_dGCh'C

Cuts at:      0     413     570 
   Size:        413     157
 
BstEII G'GTnAC_C

Cuts at:      0     270     570 
   Size:        270     300
 
BstYI r'GATC_y

Cuts at:      0     149     452     570 
   Size:        149     303     118

  Fragments arranged by size: 

                 303     149     118
 
BtgI C'CryG_G

Cuts at:      0     404     570 
   Size:        404     166
 
BtsI GCAGTG_nn'

Cuts at:      0      91     570 
   Size:         91     479
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     171     206     570 
   Size:        171      35     364

  Fragments arranged by size: 

                 364     171      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     433     570 
   Size:        433     137
 
EcoICRI GAG'CTC

Cuts at:      0     411     570 
   Size:        411     159
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      78     180     570 
   Size:         78     102     390

  Fragments arranged by size: 

                 390     102      78
 
HaeII r_GCGC'y

Cuts at:      0     265     547     570 
   Size:        265     282      23

  Fragments arranged by size: 

                 282     265      23
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      35     570 
   Size:         35     535
 
MspA1I CmG'CkG

Cuts at:      0     126     570 
   Size:        126     444
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     393     570 
   Size:        393     177
 
PfoI T'CCnGG_A

Cuts at:      0     506     570 
   Size:        506      64
 
PstI C_TGCA'G

Cuts at:      0     500     570 
   Size:        500      70
 
PvuII CAG'CTG

Cuts at:      0     126     570 
   Size:        126     444
 
SacI G_AGCT'C

Cuts at:      0     413     570 
   Size:        413     157
 
SbfI CC_TGCA'GG

Cuts at:      0     500     570 
   Size:        500      70
 
SfcI C'TryA_G

Cuts at:      0     496     570 
   Size:        496      74
 
StyI C'CwwG_G

Cuts at:      0     518     570 
   Size:        518      52
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     123     387     570 
   Size:        123     264     183

  Fragments arranged by size: 

                 264     183     123
 

 Enzymes that do cut:

     AlfI    AlwNI    BanII     BbsI    BglII     BmrI     BsaI    BsaWI
    BseRI     BsgI  BsiHKAI     BsmI Bsp1286I   BstEII    BstYI     BtgI
     BtsI    CspCI     EarI  EcoICRI     FalI    HaeII     MmeI   MspA1I
    PflMI     PfoI     PstI    PvuII     SacI     SbfI     SfcI     StyI
     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI
 Bme1580I    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaAI
    BsaBI    BsaHI    BsaXI    BseYI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FseI     FspI    FspAI    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI    SanDI     SapI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI