(Linear) (Six-base) MAPSORT of: KIBB6826.seq  Check: 1181  from: 1  to: 561



KIBB6826.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:27  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     376     481     561 
   Size:        376     105      80

  Fragments arranged by size: 

                 376     105      80
 
AlwNI CAG_nnn'CTG

Cuts at:      0     482     561 
   Size:        482      79
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     364     561 
   Size:        364     197
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     235     400     561 
   Size:        235     165     161

  Fragments arranged by size: 

                 235     165     161
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     142     561 
   Size:        142     419
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0       7     202     561 
   Size:          7     195     359

  Fragments arranged by size: 

                 359     195       7
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     406     561 
   Size:        406     155
 
BspHI T'CATG_A

Cuts at:      0     410     561 
   Size:        410     151
 
BstEII G'GTnAC_C

Cuts at:      0     547     561 
   Size:        547      14
 
Bsu36I CC'TnA_GG

Cuts at:      0     185     514     561 
   Size:        185     329      47

  Fragments arranged by size: 

                 329     185      47
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     235     376     400     481     561 
   Size:        235     141      24      81      80

  Fragments arranged by size: 

                 235     141      81      80      24
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     336     368     494     526     561 
   Size:        336      32     126      32      35

  Fragments arranged by size: 

                 336     126      35      32      32
 
MfeI C'AATT_G

Cuts at:      0     130     561 
   Size:        130     431
 
MspA1I CmG'CkG

Cuts at:      0     201     561 
   Size:        201     360
 
PfoI T'CCnGG_A

Cuts at:      0     365     561 
   Size:        365     196
 
PshAI GACnn'nnGTC

Cuts at:      0     496     561 
   Size:        496      65
 
PstI C_TGCA'G

Cuts at:      0     206     561 
   Size:        206     355
 
PvuII CAG'CTG

Cuts at:      0     201     561 
   Size:        201     360
 
SfcI C'TryA_G

Cuts at:      0      50     202     561 
   Size:         50     152     359

  Fragments arranged by size: 

                 359     152      50
 
SmlI C'TyrA_G

Cuts at:      0     157     561 
   Size:        157     404
 
StyI C'CwwG_G

Cuts at:      0     467     561 
   Size:        467      94
 
TatI w'GTAC_w

Cuts at:      0     473     561 
   Size:        473      88
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     501     561 
   Size:        501      60
 

 Enzymes that do cut:

     AcuI    AlwNI     BbsI     BpmI    BpuEI    BseRI     BsgI    BspHI
   BstEII   Bsu36I  Eco57MI    Hin4I     MfeI   MspA1I     PfoI    PshAI
     PstI    PvuII     SfcI     SmlI     StyI     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI
    BstYI  BstZ17I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MluI     MmeI     MscI
     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI
     PmlI     PpiI    PpuMI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI