(Linear) (Six-base) MAPSORT of: KIBB6825.seq  Check: 7225  from: 1  to: 495



KIBB6825.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     203     495 
   Size:        203     292
 
AflII C'TTAA_G

Cuts at:      0     223     495 
   Size:        223     272
 
ApaI G_GGCC'C

Cuts at:      0      51     495 
   Size:         51     444
 
ApoI r'AATT_y

Cuts at:      0     136     148     187     460     495 
   Size:        136      12      39     273      35

  Fragments arranged by size: 

                 273     136      39      35      12
 
BanII G_rGCy'C

Cuts at:      0      51     495 
   Size:         51     444
 
Bme1580I G_kGCm'C

Cuts at:      0      51     495 
   Size:         51     444
 
BmgBI CAC'GTC

Cuts at:      0     111     495 
   Size:        111     384
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     495 
   Size:         73     422
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     495 
   Size:         52     443
 
BsaWI w'CCGG_w

Cuts at:      0     103     495 
   Size:        103     392
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      46      55      70     495 
   Size:         46       9      15     425

  Fragments arranged by size: 

                 425      46      15       9
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     267     495 
   Size:        267     228
 
Bsp1286I G_dGCh'C

Cuts at:      0      51     495 
   Size:         51     444
 
BsrBI CCG'CTC

Cuts at:      0      45     495 
   Size:         45     450
 
BtgI C'CryG_G

Cuts at:      0      96     495 
   Size:         96     399
 
ClaI AT'CG_AT

Cuts at:      0     340     495 
   Size:        340     155
 
DraI TTT'AAA

Cuts at:      0     192     495 
   Size:        192     303
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      88     495 
   Size:         88     407
 
EaeI y'GGCC_r

Cuts at:      0     304     495 
   Size:        304     191
 
EarI CTCTTCn'nnn_

Cuts at:      0      23     495 
   Size:         23     472
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19     495 
   Size:         19     476
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     203     495 
   Size:         73     130     292

  Fragments arranged by size: 

                 292     130      73
 
EcoO109I rG'GnC_Cy

Cuts at:      0      48     495 
   Size:         48     447
 
EcoRI G'AATT_C

Cuts at:      0     148     495 
   Size:        148     347
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     408     495 
   Size:        408      87
 
HincII GTy'rAC

Cuts at:      0     213     495 
   Size:        213     282
 
HpaI GTT'AAC

Cuts at:      0     213     495 
   Size:        213     282
 
MscI TGG'CCA

Cuts at:      0     306     495 
   Size:        306     189
 
MspA1I CmG'CkG

Cuts at:      0      63      98     495 
   Size:         63      35     397

  Fragments arranged by size: 

                 397      63      35
 
PspOMI G'GGCC_C

Cuts at:      0      47     495 
   Size:         47     448
 
PvuII CAG'CTG

Cuts at:      0      63     495 
   Size:         63     432
 
SacII CC_GC'GG

Cuts at:      0      99     495 
   Size:         99     396
 
ScaI AGT'ACT

Cuts at:      0      73     495 
   Size:         73     422
 
SmlI C'TyrA_G

Cuts at:      0      67     223     495 
   Size:         67     156     272

  Fragments arranged by size: 

                 272     156      67
 
TatI w'GTAC_w

Cuts at:      0      71     495 
   Size:         71     424
 

 Enzymes that do cut:

     AcuI    AflII     ApaI     ApoI    BanII Bme1580I    BmgBI     BpmI
    BpuEI    BsaWI    BseRI     BsgI Bsp1286I    BsrBI     BtgI     ClaI
     DraI     DrdI     EaeI     EarI     EciI  Eco57MI EcoO109I    EcoRI
     FalI   HincII     HpaI     MscI   MspA1I   PspOMI    PvuII    SacII
     ScaI     SmlI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
     BmrI     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI
    BsaXI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI
    CspCI   DraIII     EagI  EcoICRI    EcoNI    EcoRV     FseI     FspI
    FspAI    HaeII    Hin4I  HindIII     KasI     KpnI     MfeI     MluI
     MmeI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI     SalI    SanDI     SapI
     SbfI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI