(Linear) (Six-base) MAPSORT of: KIBB6820.seq  Check: 9396  from: 1  to: 558



KIBB6820.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     505     558 
   Size:        505      53
 
AleI CACnn'nnGTG

Cuts at:      0     522     558 
   Size:        522      36
 
ApoI r'AATT_y

Cuts at:      0     365     467     558 
   Size:        365     102      91

  Fragments arranged by size: 

                 365     102      91
 
AvaI C'yCGr_G

Cuts at:      0      90     558 
   Size:         90     468
 
BglII A'GATC_T

Cuts at:      0     203     396     558 
   Size:        203     193     162

  Fragments arranged by size: 

                 203     193     162
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     275     558 
   Size:        275     283
 
BsmI GAATG_Cn'

Cuts at:      0     537     558 
   Size:        537      21
 
BsrBI CCG'CTC

Cuts at:      0     249     558 
   Size:        249     309
 
BssSI C'ACGA_G

Cuts at:      0      73     558 
   Size:         73     485
 
BstBI TT'CG_AA

Cuts at:      0     234     558 
   Size:        234     324
 
BstYI r'GATC_y

Cuts at:      0     203     396     558 
   Size:        203     193     162

  Fragments arranged by size: 

                 203     193     162
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     102     558 
   Size:        102     456
 
EarI CTCTTCn'nnn_

Cuts at:      0      49      50     558 
   Size:         49       1     508

  Fragments arranged by size: 

                 508      49       1
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     505     558 
   Size:        505      53
 
EcoRI G'AATT_C

Cuts at:      0     365     558 
   Size:        365     193
 
EcoRV GAT'ATC

Cuts at:      0     513     558 
   Size:        513      45
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     120     558 
   Size:        120     438
 
HaeII r_GCGC'y

Cuts at:      0      10     558 
   Size:         10     548
 
HindIII A'AGCT_T

Cuts at:      0     314     558 
   Size:        314     244
 
MslI CAynn'nnrTG

Cuts at:      0     522     558 
   Size:        522      36
 
NspI r_CATG'y

Cuts at:      0     243     558 
   Size:        243     315
 
PstI C_TGCA'G

Cuts at:      0      86     297     558 
   Size:         86     211     261

  Fragments arranged by size: 

                 261     211      86
 
SapI GCTCTTCn'nnn_

Cuts at:      0      49     558 
   Size:         49     509
 
ScaI AGT'ACT

Cuts at:      0      88     558 
   Size:         88     470
 
SfcI C'TryA_G

Cuts at:      0      82     293     558 
   Size:         82     211     265

  Fragments arranged by size: 

                 265     211      82
 
StyI C'CwwG_G

Cuts at:      0     410     558 
   Size:        410     148
 
TatI w'GTAC_w

Cuts at:      0      86     558 
   Size:         86     472
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     356     388     558 
   Size:        356      32     170

  Fragments arranged by size: 

                 356     170      32
 
XbaI T'CTAG_A

Cuts at:      0     342     547     558 
   Size:        342     205      11

  Fragments arranged by size: 

                 342     205      11
 

 Enzymes that do cut:

     AcuI     AleI     ApoI     AvaI    BglII    BseRI     BsmI    BsrBI
    BssSI    BstBI    BstYI    BtgZI     EarI  Eco57MI    EcoRI    EcoRV
     FalI    HaeII  HindIII     MslI     NspI     PstI     SapI     ScaI
     SfcI     StyI     TatI     TstI     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrDI    BsrFI    BsrGI   BssHII   BstAPI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I     FseI     FspI
    FspAI    Hin4I   HincII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII  Tth111I     XcmI     XhoI     XmaI     XmnI     ZraI