(Linear) (Six-base) MAPSORT of: KIBB6813.seq  Check: 6678  from: 1  to: 480



KIBB6813.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AccI GT'mk_AC

Cuts at:      0     302     480 
   Size:        302     178
 
AflIII A'CryG_T

Cuts at:      0     347     480 
   Size:        347     133
 
ApoI r'AATT_y

Cuts at:      0      52      87     480 
   Size:         52      35     393

  Fragments arranged by size: 

                 393      52      35
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      36      71     350     480 
   Size:         36      35     279     130

  Fragments arranged by size: 

                 279     130      36      35
 
BglII A'GATC_T

Cuts at:      0     471     480 
   Size:        471       9
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     333     480 
   Size:        333     147
 
BsiEI CG_ry'CG

Cuts at:      0      30     480 
   Size:         30     450
 
BstYI r'GATC_y

Cuts at:      0     471     480 
   Size:        471       9
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     372     407     480 
   Size:        372      35      73

  Fragments arranged by size: 

                 372      73      35
 
DraI TTT'AAA

Cuts at:      0     149     480 
   Size:        149     331
 
EaeI y'GGCC_r

Cuts at:      0     246     256     480 
   Size:        246      10     224

  Fragments arranged by size: 

                 246     224      10
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     352     384     480 
   Size:        352      32      96

  Fragments arranged by size: 

                 352      96      32
 
HincII GTy'rAC

Cuts at:      0     266     303     480 
   Size:        266      37     177

  Fragments arranged by size: 

                 266     177      37
 
HindIII A'AGCT_T

Cuts at:      0     283     480 
   Size:        283     197
 
HpaI GTT'AAC

Cuts at:      0     266     480 
   Size:        266     214
 
MscI TGG'CCA

Cuts at:      0     248     258     480 
   Size:        248      10     222

  Fragments arranged by size: 

                 248     222      10
 
MslI CAynn'nnrTG

Cuts at:      0     149     480 
   Size:        149     331
 
NspI r_CATG'y

Cuts at:      0     351     480 
   Size:        351     129
 
PciI A'CATG_T

Cuts at:      0     347     480 
   Size:        347     133
 
PvuI CG_AT'CG

Cuts at:      0      30     480 
   Size:         30     450
 
SalI G'TCGA_C

Cuts at:      0     301     480 
   Size:        301     179
 
StyI C'CwwG_G

Cuts at:      0     259     480 
   Size:        259     221
 
SwaI ATTT'AAAT

Cuts at:      0     149     480 
   Size:        149     331
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     379     480 
   Size:        379     101
 
Tth111I GACn'n_nGTC

Cuts at:      0      40     480 
   Size:         40     440
 

 Enzymes that do cut:

     AccI   AflIII     ApoI     BbsI    BglII     BmrI    BsiEI    BstYI
    CspCI     DraI     EaeI    Hin4I   HincII  HindIII     HpaI     MscI
     MslI     NspI     PciI     PvuI     SalI     StyI     SwaI    TaqII
  Tth111I

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI
 Bme1580I    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
   DraIII     DrdI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
     KasI     KpnI     MfeI     MluI     MmeI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI    PvuII    RsrII     SacI
    SacII    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     TatI     TstI     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI