Restriction map for
pF1KB6811
(Linear) (Six-base) MAPSORT of: KIBB6811.seq Check: 1491 from: 1 to: 480
KIBB6811.orf
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 454 480
Size: 454 26
AlwNI CAG_nnn'CTG
Cuts at: 0 291 303 422 480
Size: 291 12 119 58
Fragments arranged by size:
291 119 58 12
AvaI C'yCGr_G
Cuts at: 0 341 480
Size: 341 139
BanI G'GyrC_C
Cuts at: 0 328 480
Size: 328 152
BanII G_rGCy'C
Cuts at: 0 17 480
Size: 17 463
BlpI GC'TnA_GC
Cuts at: 0 89 480
Size: 89 391
Bme1580I G_kGCm'C
Cuts at: 0 331 480
Size: 331 149
BsaI GGTCTCn'nnnn_
Cuts at: 0 313 338 480
Size: 313 25 142
Fragments arranged by size:
313 142 25
BsaHI Gr'CG_yC
Cuts at: 0 383 480
Size: 383 97
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 85 202 205 208 480
Size: 85 117 3 3 272
Fragments arranged by size:
272 117 85 3 3
BsiEI CG_ry'CG
Cuts at: 0 317 480
Size: 317 163
BsiHKAI G_wGCw'C
Cuts at: 0 17 480
Size: 17 463
Bsp1286I G_dGCh'C
Cuts at: 0 17 331 480
Size: 17 314 149
Fragments arranged by size:
314 149 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 356 480
Size: 356 124
BsrBI CCG'CTC
Cuts at: 0 88 480
Size: 88 392
BsrFI r'CCGG_y
Cuts at: 0 312 480
Size: 312 168
BssSI C'ACGA_G
Cuts at: 0 16 480
Size: 16 464
BstAPI GCAn_nnn'nTGC
Cuts at: 0 45 480
Size: 45 435
BstEII G'GTnAC_C
Cuts at: 0 54 480
Size: 54 426
BtgI C'CryG_G
Cuts at: 0 62 396 480
Size: 62 334 84
Fragments arranged by size:
334 84 62
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 189 480
Size: 189 291
DrdI GACnn_nn'nnGTC
Cuts at: 0 160 480
Size: 160 320
EaeI y'GGCC_r
Cuts at: 0 83 128 176 314 413 480
Size: 83 45 48 138 99 67
Fragments arranged by size:
138 99 83 67 48 45
EagI C'GGCC_G
Cuts at: 0 314 480
Size: 314 166
EarI CTCTTCn'nnn_
Cuts at: 0 5 385 473 480
Size: 5 380 88 7
Fragments arranged by size:
380 88 7 5
EcoICRI GAG'CTC
Cuts at: 0 15 480
Size: 15 465
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 454 480
Size: 454 26
EcoO109I rG'GnC_Cy
Cuts at: 0 453 480
Size: 453 27
HaeII r_GCGC'y
Cuts at: 0 96 291 480
Size: 96 195 189
Fragments arranged by size:
195 189 96
MscI TGG'CCA
Cuts at: 0 178 415 480
Size: 178 237 65
Fragments arranged by size:
237 178 65
MspA1I CmG'CkG
Cuts at: 0 64 480
Size: 64 416
NaeI GCC'GGC
Cuts at: 0 314 480
Size: 314 166
NgoMIV G'CCGG_C
Cuts at: 0 312 480
Size: 312 168
NspI r_CATG'y
Cuts at: 0 285 480
Size: 285 195
PasI CC'CwG_GG
Cuts at: 0 324 480
Size: 324 156
PflMI CCAn_nnn'nTGG
Cuts at: 0 402 480
Size: 402 78
PpuMI rG'GwC_Cy
Cuts at: 0 453 480
Size: 453 27
SacI G_AGCT'C
Cuts at: 0 17 480
Size: 17 463
SacII CC_GC'GG
Cuts at: 0 65 480
Size: 65 415
SanDI GG'GwC_CC
Cuts at: 0 453 480
Size: 453 27
SapI GCTCTTCn'nnn_
Cuts at: 0 5 385 480
Size: 5 380 95
Fragments arranged by size:
380 95 5
SphI G_CATG'C
Cuts at: 0 285 480
Size: 285 195
Enzymes that do cut:
AcuI AlwNI AvaI BanI BanII BlpI Bme1580I BsaI
BsaHI BseRI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI
BssSI BstAPI BstEII BtgI BtgZI DrdI EaeI EagI
EarI EcoICRI Eco57MI EcoO109I HaeII MscI MspA1I NaeI
NgoMIV NspI PasI PflMI PpuMI SacI SacII SanDI
SapI SphI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaAI BsaBI
BsaWI BsaXI BseYI BsgI BsiWI BsmI BsmBI BspEI
BspHI BsrDI BsrGI BssHII BstBI BstXI BstYI BstZ17I
Bsu36I BtsI ClaI CspCI DraI DraIII EciI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MslI
NarI NcoI NdeI NheI NotI NruI NsiI PacI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SalI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SrfI SspI StuI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI