(Linear) (Six-base) MAPSORT of: KIBB6810.seq  Check: 9824  from: 1  to: 555



KIBB6810.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:27  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     293     555 
   Size:        293     262
 
AvaI C'yCGr_G

Cuts at:      0     341     555 
   Size:        341     214
 
BanII G_rGCy'C

Cuts at:      0     395     555 
   Size:        395     160
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     172     206     555 
   Size:        172      34     349

  Fragments arranged by size: 

                 349     172      34
 
Bme1580I G_kGCm'C

Cuts at:      0     469     555 
   Size:        469      86
 
BmgBI CAC'GTC

Cuts at:      0     506     555 
   Size:        506      49
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     366     555 
   Size:        366     189
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      46      61     301     555 
   Size:         46      15     240     254

  Fragments arranged by size: 

                 254     240      46      15
 
BsiEI CG_ry'CG

Cuts at:      0      84     555 
   Size:         84     471
 
BsiHKAI G_wGCw'C

Cuts at:      0     395     555 
   Size:        395     160
 
BsmI GAATG_Cn'

Cuts at:      0     510     555 
   Size:        510      45
 
Bsp1286I G_dGCh'C

Cuts at:      0     395     469     555 
   Size:        395      74      86

  Fragments arranged by size: 

                 395      86      74
 
BsrBI CCG'CTC

Cuts at:      0     360     555 
   Size:        360     195
 
BsrDI GCAATG_nn'

Cuts at:      0     188     555 
   Size:        188     367
 
BssSI C'ACGA_G

Cuts at:      0     329     555 
   Size:        329     226
 
EarI CTCTTCn'nnn_

Cuts at:      0      38      47     158     308     555 
   Size:         38       9     111     150     247

  Fragments arranged by size: 

                 247     150     111      38       9
 
EcoICRI GAG'CTC

Cuts at:      0     393     555 
   Size:        393     162
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     293     366     555 
   Size:        293      73     189

  Fragments arranged by size: 

                 293     189      73
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     382     414     555 
   Size:        382      32     141

  Fragments arranged by size: 

                 382     141      32
 
PspXI vC'TCGA_Gb

Cuts at:      0     341     555 
   Size:        341     214
 
PstI C_TGCA'G

Cuts at:      0     206     555 
   Size:        206     349
 
SacI G_AGCT'C

Cuts at:      0     395     555 
   Size:        395     160
 
SexAI A'CCwGG_T

Cuts at:      0     434     555 
   Size:        434     121
 
SfcI C'TryA_G

Cuts at:      0     202     374     555 
   Size:        202     172     181

  Fragments arranged by size: 

                 202     181     172
 
SmlI C'TyrA_G

Cuts at:      0     341     555 
   Size:        341     214
 
StyI C'CwwG_G

Cuts at:      0     446     555 
   Size:        446     109
 
Tth111I GACn'n_nGTC

Cuts at:      0     436     555 
   Size:        436     119
 
XhoI C'TCGA_G

Cuts at:      0     341     555 
   Size:        341     214
 
XmnI GAAnn'nnTTC

Cuts at:      0      74     130     555 
   Size:         74      56     425

  Fragments arranged by size: 

                 425      74      56
 

 Enzymes that do cut:

     AcuI     AvaI    BanII     BcgI Bme1580I    BmgBI     BpmI    BseRI
    BsiEI  BsiHKAI     BsmI Bsp1286I    BsrBI    BsrDI    BssSI     EarI
  EcoICRI  Eco57MI     PpiI    PspXI     PstI     SacI    SexAI     SfcI
     SmlI     StyI  Tth111I     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiWI    BsmBI
    BspEI    BspHI    BspMI    BsrFI    BsrGI   BssHII   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI    PpuMI    PshAI
     PsiI   PspOMI     PsrI     PvuI    PvuII    RsrII    SacII     SalI
    SanDI     SapI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TatI     TstI     XbaI     XcmI     XmaI     ZraI