(Linear) (Six-base) MAPSORT of: KIBB6807.seq  Check: 7706  from: 1  to: 477



KIBB6807.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     407     455     477 
   Size:        407      48      22

  Fragments arranged by size: 

                 407      48      22
 
AflII C'TTAA_G

Cuts at:      0      94     477 
   Size:         94     383
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      97     129     477 
   Size:         97      32     348

  Fragments arranged by size: 

                 348      97      32
 
ApaLI G'TGCA_C

Cuts at:      0     197     477 
   Size:        197     280
 
BanII G_rGCy'C

Cuts at:      0     347     477 
   Size:        347     130
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     268     302     477 
   Size:        268      34     175

  Fragments arranged by size: 

                 268     175      34
 
Bme1580I G_kGCm'C

Cuts at:      0     201     330     477 
   Size:        201     129     147

  Fragments arranged by size: 

                 201     147     129
 
Bpu10I CC'TnA_GC

Cuts at:      0     346     434     477 
   Size:        346      88      43

  Fragments arranged by size: 

                 346      88      43
 
BsaAI yAC'GTr

Cuts at:      0     202     477 
   Size:        202     275
 
BsaBI GATnn'nnATC

Cuts at:      0      87     477 
   Size:         87     390
 
BseYI C'CCAG_C

Cuts at:      0     417     477 
   Size:        417      60
 
BsiEI CG_ry'CG

Cuts at:      0     282     477 
   Size:        282     195
 
BsiHKAI G_wGCw'C

Cuts at:      0     201     324     477 
   Size:        201     123     153

  Fragments arranged by size: 

                 201     153     123
 
Bsp1286I G_dGCh'C

Cuts at:      0     201     324     330     347     477 
   Size:        201     123       6      17     130

  Fragments arranged by size: 

                 201     130     123      17       6
 
BsrDI GCAATG_nn'

Cuts at:      0     293     477 
   Size:        293     184
 
BsrFI r'CCGG_y

Cuts at:      0     356     477 
   Size:        356     121
 
BssSI C'ACGA_G

Cuts at:      0     323     477 
   Size:        323     154
 
BstYI r'GATC_y

Cuts at:      0     251     477 
   Size:        251     226
 
Bsu36I CC'TnA_GG

Cuts at:      0     118     477 
   Size:        118     359
 
EaeI y'GGCC_r

Cuts at:      0       2     358     477 
   Size:          2     356     119

  Fragments arranged by size: 

                 356     119       2
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     407     455     477 
   Size:        407      48      22

  Fragments arranged by size: 

                 407      48      22
 
EcoO109I rG'GnC_Cy

Cuts at:      0     156     219     456     477 
   Size:        156      63     237      21

  Fragments arranged by size: 

                 237     156      63      21
 
HindIII A'AGCT_T

Cuts at:      0     307     477 
   Size:        307     170
 
MscI TGG'CCA

Cuts at:      0       4     477 
   Size:          4     473
 
MslI CAynn'nnrTG

Cuts at:      0      53     477 
   Size:         53     424
 
NaeI GCC'GGC

Cuts at:      0     358     477 
   Size:        358     119
 
NgoMIV G'CCGG_C

Cuts at:      0     356     477 
   Size:        356     121
 
NspI r_CATG'y

Cuts at:      0     129     477 
   Size:        129     348
 
PmlI CAC'GTG

Cuts at:      0     202     477 
   Size:        202     275
 
PstI C_TGCA'G

Cuts at:      0     414     477 
   Size:        414      63
 
PvuI CG_AT'CG

Cuts at:      0     282     477 
   Size:        282     195
 
SfcI C'TryA_G

Cuts at:      0     410     477 
   Size:        410      67
 
SmlI C'TyrA_G

Cuts at:      0      94     477 
   Size:         94     383
 
StyI C'CwwG_G

Cuts at:      0     185     233     338     368     374     380     386     398 
   Size:        185      48     105      30       6       6       6      12

Cuts at:    398     477 
   Size:         79

  Fragments arranged by size: 

                 185     105      79      48      30      12       6       6
                   6
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      19      51     477 
   Size:         19      32     426

  Fragments arranged by size: 

                 426      32      19
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     307     477 
   Size:        307     170
 

 Enzymes that do cut:

     AcuI    AflII     AloI    ApaLI    BanII     BcgI Bme1580I   Bpu10I
    BsaAI    BsaBI    BseYI    BsiEI  BsiHKAI Bsp1286I    BsrDI    BsrFI
    BssSI    BstYI   Bsu36I     EaeI  Eco57MI EcoO109I  HindIII     MscI
     MslI     NaeI   NgoMIV     NspI     PmlI     PstI     PvuI     SfcI
     SmlI     StyI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI     ApaI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
    BbvCI    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmrI     BmtI     BplI     BpmI    BpuEI     BsaI    BsaHI    BsaWI
    BsaXI    BseRI     BsgI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI
  BstZ17I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI   MspA1I     NarI     NcoI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TatI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI