(Linear) (Six-base) MAPSORT of: KIBB6769.seq  Check: 7574  from: 1  to: 348



KIBB6769.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     286     348 
   Size:        286      62
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      46     348 
   Size:         46     302
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     200     205     232     237     348 
   Size:        200       5      27       5     111

  Fragments arranged by size: 

                 200     111      27       5       5
 
AlwNI CAG_nnn'CTG

Cuts at:      0     288     348 
   Size:        288      60
 
AvaI C'yCGr_G

Cuts at:      0     146     348 
   Size:        146     202
 
BanII G_rGCy'C

Cuts at:      0      88     153     348 
   Size:         88      65     195

  Fragments arranged by size: 

                 195      88      65
 
BglI GCCn_nnn'nGGC

Cuts at:      0     331     348 
   Size:        331      17
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      57     348 
   Size:         57     291
 
Bpu10I CC'TnA_GC

Cuts at:      0      82     348 
   Size:         82     266
 
BsaWI w'CCGG_w

Cuts at:      0     218     348 
   Size:        218     130
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      25     348 
   Size:         25     323
 
BseYI C'CCAG_C

Cuts at:      0     336     348 
   Size:        336      12
 
BsiHKAI G_wGCw'C

Cuts at:      0     153     348 
   Size:        153     195
 
Bsp1286I G_dGCh'C

Cuts at:      0      88     153     348 
   Size:         88      65     195

  Fragments arranged by size: 

                 195      88      65
 
BtgI C'CryG_G

Cuts at:      0      62     348 
   Size:         62     286
 
EaeI y'GGCC_r

Cuts at:      0      59     348 
   Size:         59     289
 
EarI CTCTTCn'nnn_

Cuts at:      0     314     348 
   Size:        314      34
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     157     348 
   Size:        157     191
 
EcoICRI GAG'CTC

Cuts at:      0     151     348 
   Size:        151     197
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      57     286     348 
   Size:         57     229      62

  Fragments arranged by size: 

                 229      62      57
 
EcoO109I rG'GnC_Cy

Cuts at:      0     285     333     348 
   Size:        285      48      15

  Fragments arranged by size: 

                 285      48      15
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     222     348 
   Size:        222     126
 
MscI TGG'CCA

Cuts at:      0      61     348 
   Size:         61     287
 
NcoI C'CATG_G

Cuts at:      0      62     348 
   Size:         62     286
 
NspI r_CATG'y

Cuts at:      0      45     348 
   Size:         45     303
 
PasI CC'CwG_GG

Cuts at:      0      10     288     348 
   Size:         10     278      60

  Fragments arranged by size: 

                 278      60      10
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     288     348 
   Size:        288      60
 
PstI C_TGCA'G

Cuts at:      0      25     143     348 
   Size:         25     118     205

  Fragments arranged by size: 

                 205     118      25
 
SacI G_AGCT'C

Cuts at:      0     153     348 
   Size:        153     195
 
SfcI C'TryA_G

Cuts at:      0      21     139     348 
   Size:         21     118     209

  Fragments arranged by size: 

                 209     118      21
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     331     348 
   Size:        331      17
 
SphI G_CATG'C

Cuts at:      0      45     348 
   Size:         45     303
 
StyI C'CwwG_G

Cuts at:      0      62     348 
   Size:         62     286
 

 Enzymes that do cut:

     AcuI     AlfI     AloI    AlwNI     AvaI    BanII     BglI     BpmI
   Bpu10I    BsaWI    BseRI    BseYI  BsiHKAI Bsp1286I     BtgI     EaeI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I     MmeI     MscI     NcoI
     NspI     PasI    PflMI     PstI     SacI     SfcI     SfiI     SphI
     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaXI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MslI   MspA1I
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI     SspI     StuI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI