(Linear) (Six-base) MAPSORT of: KIBB6763.seq  Check: 9166  from: 1  to: 339



KIBB6763.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     293     339 
   Size:        293      46
 
BamHI G'GATC_C

Cuts at:      0      52     339 
   Size:         52     287
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      57     281     339 
   Size:         57     224      58

  Fragments arranged by size: 

                 224      58      57
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      12     339 
   Size:         12     327
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     144     339 
   Size:        144     195
 
BsiHKAI G_wGCw'C

Cuts at:      0     232     339 
   Size:        232     107
 
Bsp1286I G_dGCh'C

Cuts at:      0     232     339 
   Size:        232     107
 
BspHI T'CATG_A

Cuts at:      0     197     339 
   Size:        197     142
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     227     339 
   Size:        227     112
 
BstYI r'GATC_y

Cuts at:      0      52     339 
   Size:         52     287
 
BtgI C'CryG_G

Cuts at:      0      11     339 
   Size:         11     328
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     270     305     339 
   Size:        270      35      34

  Fragments arranged by size: 

                 270      35      34
 
HindIII A'AGCT_T

Cuts at:      0     103     339 
   Size:        103     236
 
PfoI T'CCnGG_A

Cuts at:      0     294     339 
   Size:        294      45
 
StyI C'CwwG_G

Cuts at:      0      48     339 
   Size:         48     291
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     167     199     339 
   Size:        167      32     140

  Fragments arranged by size: 

                 167     140      32
 

 Enzymes that do cut:

     AlfI    BamHI     BmrI     BsaI     BsgI  BsiHKAI Bsp1286I    BspHI
   BstAPI    BstYI     BtgI    CspCI  HindIII     PfoI     StyI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI
   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI