Restriction map for
pF1KB6756
(Linear) (Six-base) MAPSORT of: KIBB6756.seq Check: 5266 from: 1 to: 438
KIBB6756.orf
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AflIII A'CryG_T
Cuts at: 0 257 438
Size: 257 181
AlwNI CAG_nnn'CTG
Cuts at: 0 106 438
Size: 106 332
BanII G_rGCy'C
Cuts at: 0 137 438
Size: 137 301
BbsI GAAGACnn'nnnn_
Cuts at: 0 289 438
Size: 289 149
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 258 292 438
Size: 258 34 146
Fragments arranged by size:
258 146 34
BclI T'GATC_A
Cuts at: 0 251 438
Size: 251 187
BfrBI ATG'CAT
Cuts at: 0 3 438
Size: 3 435
BglI GCCn_nnn'nGGC
Cuts at: 0 86 438
Size: 86 352
BlpI GC'TnA_GC
Cuts at: 0 298 438
Size: 298 140
BmrI ACTGGGnnnn_n'
Cuts at: 0 43 372 438
Size: 43 329 66
Fragments arranged by size:
329 66 43
BsaI GGTCTCn'nnnn_
Cuts at: 0 327 438
Size: 327 111
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1 157 438
Size: 1 156 281
Fragments arranged by size:
281 156 1
BseYI C'CCAG_C
Cuts at: 0 328 438
Size: 328 110
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 254 438
Size: 254 184
BsiHKAI G_wGCw'C
Cuts at: 0 137 438
Size: 137 301
Bsp1286I G_dGCh'C
Cuts at: 0 137 438
Size: 137 301
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 401 438
Size: 401 37
BsrBI CCG'CTC
Cuts at: 0 9 180 302 438
Size: 9 171 122 136
Fragments arranged by size:
171 136 122 9
BsrDI GCAATG_nn'
Cuts at: 0 65 192 438
Size: 65 127 246
Fragments arranged by size:
246 127 65
Bsu36I CC'TnA_GG
Cuts at: 0 127 438
Size: 127 311
BtgI C'CryG_G
Cuts at: 0 59 438
Size: 59 379
BtsI GCAGTG_nn'
Cuts at: 0 276 438
Size: 276 162
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 48 83 438
Size: 48 35 355
Fragments arranged by size:
355 48 35
DraI TTT'AAA
Cuts at: 0 407 438
Size: 407 31
EcoICRI GAG'CTC
Cuts at: 0 135 438
Size: 135 303
EcoO109I rG'GnC_Cy
Cuts at: 0 103 390 438
Size: 103 287 48
Fragments arranged by size:
287 103 48
HaeII r_GCGC'y
Cuts at: 0 49 438
Size: 49 389
HincII GTy'rAC
Cuts at: 0 56 153 438
Size: 56 97 285
Fragments arranged by size:
285 97 56
MspA1I CmG'CkG
Cuts at: 0 220 328 438
Size: 220 108 110
Fragments arranged by size:
220 110 108
NruI TCG'CGA
Cuts at: 0 422 438
Size: 422 16
NsiI A_TGCA'T
Cuts at: 0 5 438
Size: 5 433
NspI r_CATG'y
Cuts at: 0 228 352 438
Size: 228 124 86
Fragments arranged by size:
228 124 86
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 415 438
Size: 415 23
PpuMI rG'GwC_Cy
Cuts at: 0 390 438
Size: 390 48
PshAI GACnn'nnGTC
Cuts at: 0 61 438
Size: 61 377
PstI C_TGCA'G
Cuts at: 0 271 438
Size: 271 167
PvuII CAG'CTG
Cuts at: 0 220 328 438
Size: 220 108 110
Fragments arranged by size:
220 110 108
SacI G_AGCT'C
Cuts at: 0 137 438
Size: 137 301
SfcI C'TryA_G
Cuts at: 0 119 267 438
Size: 119 148 171
Fragments arranged by size:
171 148 119
StyI C'CwwG_G
Cuts at: 0 81 438
Size: 81 357
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 175 438
Size: 175 263
TatI w'GTAC_w
Cuts at: 0 227 438
Size: 227 211
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 369 438
Size: 369 69
Enzymes that do cut:
AflIII AlwNI BanII BbsI BcgI BclI BfrBI BglI
BlpI BmrI BsaI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspMI BsrBI BsrDI Bsu36I BtgI BtsI CspCI DraI
EcoICRI EcoO109I HaeII HincII MspA1I NruI NsiI NspI
PpiI PpuMI PshAI PstI PvuII SacI SfcI StyI
TaqII TatI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BbeI BbvCI BciVI BglII Bme1580I BmgBI BmtI BplI
BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgZI ClaI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI NaeI NarI NcoI NdeI NgoMIV NheI
NotI PacI PasI PciI PflMI PfoI PmeI PmlI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI