(Linear) (Six-base) MAPSORT of: KIBB6751.seq  Check: 3457  from: 1  to: 312



KIBB6751.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AfeI AGC'GCT

Cuts at:      0      27     312 
   Size:         27     285
 
AflIII A'CryG_T

Cuts at:      0     203     312 
   Size:        203     109
 
AlwNI CAG_nnn'CTG

Cuts at:      0      48     312 
   Size:         48     264
 
BbvCI CC'TCA_GC

Cuts at:      0      29     312 
   Size:         29     283
 
BglI GCCn_nnn'nGGC

Cuts at:      0     166     312 
   Size:        166     146
 
BlpI GC'TnA_GC

Cuts at:      0      22     229     312 
   Size:         22     207      83

  Fragments arranged by size: 

                 207      83      22
 
Bpu10I CC'TnA_GC

Cuts at:      0      29     312 
   Size:         29     283
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      46     312 
   Size:         46     266
 
BsiHKAI G_wGCw'C

Cuts at:      0     235     312 
   Size:        235      77
 
Bsp1286I G_dGCh'C

Cuts at:      0     235     312 
   Size:        235      77
 
BspHI T'CATG_A

Cuts at:      0     146     312 
   Size:        146     166
 
BsrFI r'CCGG_y

Cuts at:      0     247     312 
   Size:        247      65
 
BsrGI T'GTAC_A

Cuts at:      0     206     312 
   Size:        206     106
 
Bsu36I CC'TnA_GG

Cuts at:      0      58     312 
   Size:         58     254
 
BtsI GCAGTG_nn'

Cuts at:      0     305     312 
   Size:        305       7
 
EaeI y'GGCC_r

Cuts at:      0     275     312 
   Size:        275      37
 
EcoO109I rG'GnC_Cy

Cuts at:      0     134     312 
   Size:        134     178
 
HaeII r_GCGC'y

Cuts at:      0      29     312 
   Size:         29     283
 
MscI TGG'CCA

Cuts at:      0     277     312 
   Size:        277      35
 
MslI CAynn'nnrTG

Cuts at:      0     242     312 
   Size:        242      70
 
MspA1I CmG'CkG

Cuts at:      0      45     312 
   Size:         45     267
 
NaeI GCC'GGC

Cuts at:      0     249     312 
   Size:        249      63
 
NgoMIV G'CCGG_C

Cuts at:      0     247     312 
   Size:        247      65
 
PstI C_TGCA'G

Cuts at:      0     167     312 
   Size:        167     145
 
PvuII CAG'CTG

Cuts at:      0      45     312 
   Size:         45     267
 
SbfI CC_TGCA'GG

Cuts at:      0     167     312 
   Size:        167     145
 
SfcI C'TryA_G

Cuts at:      0     163     312 
   Size:        163     149
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     250     312 
   Size:        250      62
 
TatI w'GTAC_w

Cuts at:      0     206     312 
   Size:        206     106
 

 Enzymes that do cut:

     AfeI   AflIII    AlwNI    BbvCI     BglI     BlpI   Bpu10I    BseRI
  BsiHKAI Bsp1286I    BspHI    BsrFI    BsrGI   Bsu36I     BtsI     EaeI
 EcoO109I    HaeII     MscI     MslI   MspA1I     NaeI   NgoMIV     PstI
    PvuII     SbfI     SfcI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI    BglII Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrDI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I     BtgI    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     NarI     NcoI     NdeI     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI