(Linear) (Six-base) MAPSORT of: KIBB6743.seq  Check: 2037  from: 1  to: 297



KIBB6743.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     106     297 
   Size:        106     191
 
BanII G_rGCy'C

Cuts at:      0      65     297 
   Size:         65     232
 
BglII A'GATC_T

Cuts at:      0     125     218     297 
   Size:        125      93      79

  Fragments arranged by size: 

                 125      93      79
 
BlpI GC'TnA_GC

Cuts at:      0      68     297 
   Size:         68     229
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      85     297 
   Size:         85     212
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     270     297 
   Size:        270      27
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     154     297 
   Size:        154     143
 
Bsp1286I G_dGCh'C

Cuts at:      0      65     297 
   Size:         65     232
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     106     297 
   Size:        106     191
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      85     297 
   Size:         85     212
 
BstYI r'GATC_y

Cuts at:      0     125     218     297 
   Size:        125      93      79

  Fragments arranged by size: 

                 125      93      79
 
BtgI C'CryG_G

Cuts at:      0     257     297 
   Size:        257      40
 
EaeI y'GGCC_r

Cuts at:      0     209     297 
   Size:        209      88
 
MscI TGG'CCA

Cuts at:      0     211     297 
   Size:        211      86
 
NcoI C'CATG_G

Cuts at:      0     257     297 
   Size:        257      40
 
NruI TCG'CGA

Cuts at:      0     145     297 
   Size:        145     152
 
PsiI TTA'TAA

Cuts at:      0     107     297 
   Size:        107     190
 
SfcI C'TryA_G

Cuts at:      0     150     297 
   Size:        150     147
 
StyI C'CwwG_G

Cuts at:      0      57     212     257     297 
   Size:         57     155      45      40

  Fragments arranged by size: 

                 155      57      45      40
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      36      68     297 
   Size:         36      32     229

  Fragments arranged by size: 

                 229      36      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     264     297 
   Size:        264      33
 

 Enzymes that do cut:

     AarI    BanII    BglII     BlpI     BmrI    BsaXI     BsgI Bsp1286I
    BspMI   BstAPI    BstYI     BtgI     EaeI     MscI     NcoI     NruI
     PsiI     SfcI     StyI     TstI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI Bme1580I    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MslI
   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TatI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI